Niger_00090 : CDS information

Location

Organism	Streptomyces violaceusniger
Strain	DSM 4137
Entry name	Nigericin
Contig	DQ354110
Start / Stop / Direction	58,370 / 59,629 / + [in whole cluster] 58,370 / 59,629 / + [in contig]
Location	58370..59629 [in whole cluster] 58370..59629 [in contig]
Type	CDS
Length	1,260 bp (419 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	NigD
Gene
Gene (GenBank)	nigD
EC number
Keyword	C-30 hydroxylate
Note
Note (GenBank)	cytochrome P450 mono-oxygenase; ORF3
Reference	ACC Q27W66 PmId [17584617] Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137. (Chem Biol. , 2007) comment Streptomyces sp. DSM4137におけるNigericin Biosynthetic Gene Clusterに関する文献。 nigD(419 aa): cytochrome P450 nigD自体の機能実験は行われておらず、相同性よりcytochrome P450と予想している。特にnanchangmycin(C-30)やmonensin A(C-26)のterminal methyl groupsを酸化するcytochrome P450と相同性が高いことが記されている。また、nigericin-producing strainをcytochrome P450 mono-oxygenaseの阻害剤の存在下で培養した際にgrisorixin (C-30-dehydroxylnigericin)が蓄積した結果を以前出していることが記載されている。(PMID: 1368213) [PMID: 1368213]で使用されているのはStreptomyces sp. RK-955.
Related Reference	ACC Q846W4 NITE Monen_00270 PmId [8017935] Production of 26-deoxymonensins A and B by Streptomyces cinnamonensis in the presence of Metyrapone. (Appl Environ Microbiol. , 1994) comment <Monensin hydroxylase(monD), Streptomyces cinnamonensis, BLAST 2; id=63%> S. cinnamonensisをcytochrome P450 enzymesの強力な特異的inhibitorであるmetyrapone存在下で培養した時、C-26 dehydroxy-monensinsが形成されたことが示されている。
Related Reference	PmId [1368213] Accumulation of grisorixin caused by treating a nigericin-producing strain with a P-450 inhibitor. (Biosci Biotechnol Biochem. , 1992) comment [PMID: 17584617]で引用されている実験報告。 Streptomyces sp. RK-955使用。該当UniProt entryなし。 nigericin-producing strainをcytochrome P450 mono-oxygenaseの阻害剤の存在下で培養した際にgrisorixin (C-30-dehydroxylnigericin)が蓄積。

Sequence

>putative cytochrome P450 [NigD]
MYPPESRFAHVENIDLTDLSFWAQPYGDRDAAFAALRRRPEPVLFQEPVMPGFGQGSGYY
AMVGHADIAEVSRRPQDFGSLPTAISIIDLPEEFNSFFGSMINMDNPQHARLRRLVSRAF
GRGMAAEFEAASHKAARKIVDDLIAKGPCDFVREVAAIMPIAVLSGMMGIPDGDYEFIYD
RSNIIVGTFDPEYVPRADQATAALLKTSHELADYIARLAADRTRNPTGDLITRLVQAQID
GERLSPEELSSFFILLVVAGMETTRNAISRGLVLLTEHPEQRKLLLSDFEAYAPGAVEEI
LRVATPINWMRRTVKRDCEVNGHRFREGEKLLLFYWSANRDESVFTDPYQFDITRDPNPH
MTFGSVGPHFCLGAHLARMEVTVLFRELFSRLPEIRTVGEPRRLVASFVEAIKHVECAF

>putative cytochrome P450 [NigD]
GTGTATCCCCCGGAATCCAGATTCGCCCATGTCGAGAACATCGACCTCACCGACTTGTCC
TTCTGGGCCCAGCCCTACGGCGATCGTGACGCCGCTTTCGCCGCGCTGCGCCGGCGGCCT
GAGCCCGTGCTCTTCCAGGAGCCGGTCATGCCCGGGTTCGGGCAGGGATCCGGCTATTAC
GCGATGGTCGGACACGCCGATATCGCCGAGGTGAGCAGGCGTCCACAGGACTTCGGCTCC
CTGCCCACCGCGATCAGCATCATCGATCTGCCGGAGGAGTTCAACAGCTTCTTCGGGTCG
ATGATCAATATGGACAATCCCCAGCACGCCCGGCTGCGCCGGCTGGTGTCCCGCGCGTTC
GGCCGGGGAATGGCCGCCGAGTTCGAGGCGGCCTCCCACAAGGCGGCCCGGAAGATTGTC
GACGACCTCATCGCGAAAGGGCCCTGTGACTTCGTCCGGGAGGTCGCCGCCATCATGCCG
ATCGCGGTGCTCAGCGGCATGATGGGGATTCCCGACGGCGACTACGAGTTCATTTATGAC
CGGTCCAATATCATCGTCGGCACCTTCGACCCCGAATATGTTCCGCGGGCCGATCAGGCC
ACCGCCGCCCTGCTGAAGACCTCACATGAGCTCGCCGACTACATCGCACGGCTCGCCGCC
GACCGCACCCGTAACCCCACCGGGGATCTGATCACCCGGCTGGTGCAGGCGCAGATCGAC
GGCGAGCGGCTCAGCCCCGAAGAGCTCTCGTCCTTCTTCATCCTGCTCGTCGTCGCCGGA
ATGGAGACGACCCGCAACGCCATCTCCCGCGGTCTGGTGCTGCTCACCGAGCATCCGGAG
CAACGGAAGCTGCTGCTCTCCGACTTCGAGGCATACGCACCGGGCGCGGTCGAGGAGATC
CTGCGGGTCGCGACCCCCATCAACTGGATGCGCCGGACCGTCAAGCGGGACTGCGAGGTG
AACGGCCACCGCTTCCGGGAGGGCGAGAAACTGCTGCTCTTCTACTGGTCCGCCAACCGG
GACGAGAGCGTCTTCACCGACCCCTATCAGTTCGACATCACCCGGGACCCCAATCCGCAT
ATGACCTTCGGATCGGTCGGCCCGCACTTCTGCCTGGGCGCCCATCTCGCCCGGATGGAG
GTCACGGTGCTCTTCCGCGAGCTGTTCAGCCGGCTGCCCGAGATCCGCACGGTGGGGGAG
CCCCGGCGACTGGTGGCCAGCTTCGTCGAGGCCATCAAGCATGTGGAGTGCGCTTTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 SSF48264 Cytochrome_P450 G3DSA:1.10.630.10 Cyt_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450
SignalP	No significant hit
TMHMM	No significant hit

Niger_00080 Niger_00100