Niger_00120 : CDS information

Location

Organism	Streptomyces violaceusniger
Strain	DSM 4137
Entry name	Nigericin
Contig	DQ354110
Start / Stop / Direction	65,478 / 64,048 / - [in whole cluster] 65,478 / 64,048 / - [in contig]
Location	complement(64048..65478) [in whole cluster] complement(64048..65478) [in contig]
Type	CDS
Length	1,431 bp (476 aa)

Annotation

Category	3.3 modification reduction
Product	putative epoxidase
Product (GenBank)	NigCI
Gene
Gene (GenBank)	nigCI
EC number
Keyword
Note
Note (GenBank)	epoxidase; ORF6
Reference	ACC Q27W63 PmId [17584617] Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137. (Chem Biol. , 2007) comment Streptomyces sp. DSM4137におけるNigericin Biosynthetic Gene Clusterに関する文献。 nigCI (476 aa): epoxidase nigCI 自体の機能実験は行われておらず、monesin biosynthesis (Streptomyces cinnamonensis)のmonCI との相同性よりepoxidaseと予想している。
Related Reference	ACC Q846W9 NITE Monen_00220 PmId [12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003) comment <Monensin epoxidase(monC1), Streptomyces cinnamonensis, BLAST 3; id=60%> Streptomyces cinnamonensisのmonensin biosynthetic gene clusterにおけるmonB と monCの機能について調べた文献。 monCI について、S. coelicolorにおけるHeterologous expressionを行ったところ、linaloolをlinalool oxideにepoxidizeする能力が10-20倍に増加することを確認している。

Sequence

>putative epoxidase [NigCI]
MTEPKHGVVLGGGWAGMLAAHVLARHLERVTVVERDVLPDGPRHRKGSPQGRHVHVLWSS
GARIVDTLLPGMIDQLLDAGARRIGFHQDLVTLTSHGWQHRFPPRQYALMCTRPFLDWKV
RDRVLATGRITLRQRAEILDLAGDAKRVTGVRVRDMGSGAAETLAADLVIDASGRGSRLR
HWLSALDVPPLEEDIVDAGIAYATRVYQGPPGAAAGFPAVNVAADHRLREPGRFGVVYPQ
EDLTWMVTLSCTRGAGLPTHDDEFLPYARTLRHPLVADLIALAKPLTSVAVSRVGANRRL
YPERLDIWPEGLLVLGDALAAFNPIYGHGMSSAARAAAALDTELQRSGTAEGTTRRAQQA
ISATVDDPWIMAASKDVEYVNCRMNTTDPRLTGGAVARQRFSDLIADRSIRSSAVCDVVT
DVISLTAPQSELASSGFLSLMHKDRVLPELTEPPLTADELALVNLSPRSAVSAGGS

>putative epoxidase [NigCI]
ATGACCGAACCCAAACACGGAGTCGTTCTGGGCGGAGGCTGGGCGGGAATGCTGGCTGCC
CATGTGCTGGCCCGCCATCTGGAGCGCGTCACCGTCGTGGAGCGCGATGTGCTGCCGGAC
GGCCCCCGGCACCGCAAGGGATCGCCGCAGGGGCGCCATGTGCATGTGCTGTGGTCCAGC
GGTGCGCGCATCGTGGACACGCTGCTGCCGGGCATGATCGACCAGTTGCTCGACGCGGGC
GCCCGCCGGATCGGTTTCCATCAGGACCTGGTGACCTTGACGTCCCACGGCTGGCAGCAC
CGCTTTCCACCGCGGCAGTACGCGCTGATGTGTACCCGCCCGTTTCTGGACTGGAAGGTC
CGTGACCGCGTTCTGGCCACCGGGCGGATCACCCTGCGCCAGCGCGCCGAGATCCTCGAT
CTGGCCGGCGACGCCAAACGGGTCACCGGGGTCCGGGTGCGCGACATGGGCAGCGGTGCG
GCGGAAACGCTGGCGGCCGATCTGGTGATCGACGCCTCGGGGCGCGGTTCCCGGCTCAGG
CACTGGCTGTCGGCGCTTGATGTGCCGCCGCTGGAGGAGGACATCGTCGACGCGGGCATC
GCGTACGCCACCCGCGTGTACCAGGGGCCGCCGGGTGCCGCCGCCGGGTTCCCTGCCGTC
AACGTGGCCGCCGACCACCGGCTGCGCGAGCCGGGCCGGTTCGGGGTGGTGTATCCGCAG
GAGGACCTCACCTGGATGGTGACCCTGTCGTGCACCCGGGGCGCCGGGCTGCCCACCCAC
GACGACGAGTTTCTGCCCTACGCCCGGACGCTGCGCCATCCGCTGGTCGCGGATCTCATC
GCGCTGGCGAAGCCGCTGACCTCGGTGGCCGTCTCGCGGGTCGGCGCCAACCGGCGGCTG
TATCCGGAGCGGCTGGACATCTGGCCGGAGGGGCTGCTGGTGCTCGGGGACGCGCTGGCC
GCGTTCAACCCGATCTACGGCCATGGCATGAGCTCCGCGGCTCGCGCGGCCGCCGCGCTG
GACACCGAGCTCCAGCGGTCCGGAACCGCCGAGGGGACGACCCGCCGGGCGCAGCAGGCG
ATCAGCGCCACCGTGGACGACCCGTGGATCATGGCCGCGTCCAAGGACGTGGAGTATGTC
AACTGCCGGATGAACACGACGGATCCGCGGCTGACCGGCGGGGCCGTGGCGCGGCAGCGG
TTCTCCGATCTGATCGCCGACCGGTCGATCCGCTCCTCGGCGGTGTGTGATGTGGTCACC
GATGTCATCAGCCTGACCGCGCCGCAGTCCGAGCTGGCGTCCAGCGGCTTTCTGTCGCTG
ATGCACAAGGACCGGGTCCTTCCGGAGCTCACCGAGCCACCGCTGACCGCCGATGAGCTC
GCGCTGGTGAACCTGAGTCCGCGCAGCGCGGTGAGTGCGGGCGGCTCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Eukaryota
TMHMM	No significant hit

Niger_00110 Niger_00130