Nogl_00120 Nogl_00120   Nogl_00140 Nogl_00140

Nogl_00130 : CDS information

close this sectionLocation

Organism
StrainATCC 27451 (=NBRC 13445)
Entry nameNogalamycin
Contig
Start / Stop / Direction14,126 / 13,281 / - [in whole cluster]
1,489 / 644 / - [in contig]
Locationcomplement(13281..14126) [in whole cluster]
complement(644..1489) [in contig]
TypeCDS
Length846 bp (281 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative nogalaviketone C-7 ketoreductase
Product (GenBank)C-7 ketoreductase
Gene
Gene (GenBank)snoaF
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11683270] The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures. (Mol Genet Genomics. , 2001)
comment
S. nogalater genomic DNA fragmentの配列解析と、異種性発現による産物解析。
nogalamycin生合成に関する20ORFsが明らかになった。

snoaF: C7-ketoreductase

aklaviketoneを蓄積するS.galilaeus H036 mutantは、snoaFをpromoter下においたplasmid pSYE57によって異種性に相補される。
SnoaFによるaklaviketone→aklavinoneへの変換は、aglyconへsugar moietyが付着できるようにし、aklavinone glycosidesを蓄積するようになる。

nogalamycin生合成経路での割り当ては、
nogalaviketone → nogalamycinone へのC-7還元が提唱されている。
nogalaviketoneとaklaviketoneの違いはstarter(acetate or propionate)のみ。
Related Reference
ACC
Q53882
NITE
Dauno_00060
PmId
[8655529] Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5. (J Bacteriol. , 1996)
comment
Blast 9th, id60%, 3e-66
Streptomyces sp. (strain C5)_dauE
Aklaviketone reductase

recombinant DauE を含んだStreptomyces lividans TK24 の抽出液は、
aklaviketone → aklavinone
maggiemycin → epsilon-rhodomycinone(RHO)
7-oxodaunomycinone → daunomycinone
へのNADPH-specificな変換を触媒した。
これらの変換はすべてposition 7でのketo基の還元。

close this sectionSequence

selected fasta
>putative nogalaviketone C-7 ketoreductase [C-7 ketoreductase]
MRAMTDSTGPRPVPAMSPAPSPTPSPGPAPGSEPAPLAVIVTGGGSGIGRATARAFAAQG
AKVLVVGRTEDALAQTAEGCADMRVLVADVASPDGPQAVVNAALREFGRIDVLVNNAAVA
GMETLQTVDRDAVARQFGTNLTAPLFLVQSALGALEKSRGIVVNVGTAATLGLRAAPTGA
LYGASKVALDYLTRTWAVELAPRGIRVVGVAPGVIDTGIGVRMGMTPEGYREFLTGMGGR
VPVGRVGRPEDVAWWIVQLARPEAGYATGMVVPVDGGLSLV
selected fasta
>putative nogalaviketone C-7 ketoreductase [C-7 ketoreductase]
GTGCGAGCCATGACTGACTCGACAGGTCCCCGCCCGGTGCCCGCCATGTCACCCGCCCCC
AGCCCCACGCCTTCCCCCGGCCCCGCCCCCGGGAGCGAACCCGCGCCGCTCGCCGTGATC
GTCACCGGCGGCGGTTCGGGTATCGGCCGGGCCACCGCCCGCGCCTTCGCCGCTCAGGGT
GCGAAGGTGCTCGTCGTCGGCCGTACCGAGGACGCGCTCGCGCAGACCGCCGAGGGCTGT
GCGGACATGCGTGTGCTCGTCGCCGACGTGGCCTCGCCCGACGGGCCGCAGGCGGTCGTC
AACGCCGCCCTGCGGGAGTTCGGGAGGATCGACGTCCTGGTCAACAACGCTGCCGTGGCG
GGCATGGAGACCCTGCAGACCGTCGACCGGGACGCCGTGGCACGGCAGTTCGGCACCAAT
CTGACGGCTCCCCTCTTCCTCGTCCAGTCCGCACTCGGCGCGCTGGAGAAGTCGCGCGGC
ATCGTCGTCAACGTGGGGACCGCCGCGACCCTGGGCCTGCGCGCCGCCCCGACCGGCGCG
CTGTACGGGGCGAGCAAGGTGGCCCTCGACTACCTGACCCGGACCTGGGCCGTCGAACTG
GCCCCCCGGGGCATCCGTGTCGTCGGCGTGGCACCCGGGGTGATCGACACGGGCATCGGC
GTCCGCATGGGCATGACCCCGGAGGGCTACCGGGAGTTCCTGACCGGCATGGGCGGCAGG
GTGCCCGTGGGCCGGGTCGGCCGTCCGGAGGACGTGGCCTGGTGGATCGTCCAGCTCGCC
CGCCCGGAGGCCGGCTACGCGACGGGCATGGTCGTCCCCGTCGACGGCGGGCTGTCGCTG
GTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[108-119] 1.69999922284049e-09 PR00080 [160-168] 1.69999922284049e-09 PR00080 [182-201] 1.69999922284049e-09 PR00080
PR00080   SDRFAMILY
[38-200] 7.59999999999998e-26 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[38-55] 6.89998448199219e-36 PR00081 [108-119] 6.89998448199219e-36 PR00081 [154-170] 6.89998448199219e-36 PR00081 [182-201] 6.89998448199219e-36 PR00081 [203-220] 6.89998448199219e-36 PR00081 [242-262] 6.89998448199219e-36 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[39-280] 5.50000000000008e-72 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[169-197] PS00061
PS00061   ADH_SHORT
SignalP
[1-15] 0.535 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Nogl_00120 Nogl_00120   Nogl_00140 Nogl_00140
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