Nogl_00260 : CDS information

Location

Organism	Streptomyces nogalater
Strain	ATCC 27451 (=NBRC 13445)
Entry name	Nogalamycin
Contig	AF187532
Start / Stop / Direction	25,881 / 26,597 / + [in whole cluster] 13,244 / 13,960 / + [in contig]
Location	25881..26597 [in whole cluster] 13244..13960 [in contig]
Type	CDS
Length	717 bp (238 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose N-methyltransferase
Product (GenBank)	putative amino methylase
Gene
Gene (GenBank)	snogA
EC number	2.1.1.-
Keyword	nogalamine
Note
Note (GenBank)
Reference	ACC Q9RN53 PmId [11683270] The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures. (Mol Genet Genomics. , 2001) comment S. nogalater genomic DNA fragmentの配列解析と、異種性発現による産物解析。 nogalamycin生合成に関する20ORFsが明らかになった。 snogA: N-methyltransferase 配列解析のみ。既に同定されているsnogX(snoX)と共にnogalamineのamino基をdimethylateするのかもしれないと言っているが、他のhomologuesでは1geneがdimethylateを担っていることも気にしている。
Related Reference	ACC Q9ZGH6 NITE Pikro_00100 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) [12119032] Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine. (Biochemistry. , 2002) comment Blast 14th, id55%, 1e-65 Streptomyces venezuelae_desVI N,N-dimethyltransferase --- [PMID: 9770448](1998) 12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。 DesVI: N,N-Dimethyltransferase desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。 desVI のDeletion/replacement mutationにより、methymycin, neomethymycin, narbomycin, and pikromycinは非産生。それらのaglyconeである10-deoxymethynolide and narbonolideを蓄積。 --- [PMID: 12119032](2002)abstract N,N-dimethyltransferasesであるtylM1 and desVI の過剰発現、精製、触媒機能確認。 TylM1 and DesVI はhomodimeric proteinで、ほとんど同じ生化学的特性を持つ。

Sequence

>putative NDP-hexose N-methyltransferase [putative amino methylase]
MYGRELADVYEMVYRSRGKSWADEAERVTAEIRSRRPGARSLLDVACGTGAHLEAFRGLF
AHTEGLELSDEMRALAERRLPGVPVRPGDMRDFALSGRFDAVVCLFCSIGYLETVADMRA
AVRTMAAHLVPGGVLVVEPWWFPERFLEGYVAGDLARGEGRTVARVSHSTRQGRRTRMEV
RFLVGEATGIREFTEIDLLTLFTREEYLAAFEDAGCPAEFLDDGLTGRGLFVGVRGAG

>putative NDP-hexose N-methyltransferase [putative amino methylase]
GTGTACGGCCGGGAACTGGCGGACGTGTACGAGATGGTCTACCGCAGCCGGGGAAAGAGC
TGGGCGGACGAGGCCGAACGGGTCACCGCGGAGATCAGGTCCCGTCGGCCCGGGGCGCGT
TCGCTGCTCGACGTCGCCTGCGGCACCGGCGCCCATCTGGAGGCCTTCCGCGGGCTGTTC
GCGCACACGGAGGGCCTGGAGCTGTCGGACGAGATGCGGGCCCTGGCCGAACGGCGGCTG
CCGGGGGTGCCGGTGCGGCCCGGGGACATGCGCGACTTCGCCCTGAGCGGGCGTTTCGAC
GCCGTGGTCTGCCTGTTCTGCTCCATCGGGTATCTGGAGACCGTCGCGGACATGCGGGCG
GCGGTGCGCACGATGGCCGCGCACCTGGTGCCGGGCGGCGTGCTCGTGGTGGAGCCCTGG
TGGTTCCCCGAGCGGTTCCTGGAGGGCTACGTCGCCGGGGACCTGGCACGCGGTGAGGGC
CGGACCGTGGCCCGGGTGTCCCACTCCACCAGGCAGGGCCGCAGGACCCGCATGGAGGTG
CGGTTCCTGGTCGGCGAGGCCACCGGGATACGCGAGTTCACGGAGATCGACCTGCTCACC
CTGTTCACCAGGGAGGAGTACCTCGCCGCGTTCGAGGACGCGGGCTGCCCGGCCGAGTTC
CTGGACGACGGGCTGACCGGCCGGGGGTTGTTCGTCGGGGTGCGCGGGGCCGGGTAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Nogl_00250 Nogl_00270