Nogl_00260 Nogl_00260   Nogl_00280 Nogl_00280

Nogl_00270 : CDS information

close this sectionLocation

Organism
StrainATCC 27451 (=NBRC 13445)
Entry nameNogalamycin
Contig
Start / Stop / Direction27,511 / 26,630 / - [in whole cluster]
14,874 / 13,993 / - [in contig]
Locationcomplement(26630..27511) [in whole cluster]
complement(13993..14874) [in contig]
TypeCDS
Length882 bp (293 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative glucose-1-phosphate thymidylyltransferase
putative dTDP-glucose synthase
Product (GenBank)putative dTDP-glucose synthase
Gene
Gene (GenBank)snogJ
EC number2.7.7.24
Keyword
  • nogalamine
  • nogalose
Note
Note (GenBank)
Reference
ACC
PmId
[11683270] The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures. (Mol Genet Genomics. , 2001)
comment
S. nogalater genomic DNA fragmentの配列解析と、異種性発現による産物解析。
nogalamycin生合成に関する20ORFsが明らかになった。

snogJ: dTDP-glucose synthase

Plasmid pSYE61(snogA, gJ, gI)は、aknYに変異がありsugar moietyを含まないaklavinoneを蓄積するS.galilaeus mutant H063を相補し、aklavinone glycosidesを産生できるようにする。
Related Reference
ACC
Q9AGY4
PmId
[12147463] Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-L-rhamnose biosynthesis in the gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155. (Appl Environ Microbiol. , 2002)
comment
Blast 44th, id58%, 3e-98
Aneurinibacillus thermoaerophilus_rmlA
Glucose-1-phosphate thymidylyltransferase(EC 2.7.7.24)

Aneurinibacillus thermoaerophilusにおいて、Glucose-1-phosphate thymidylyltransferase (rmlA)を
E. coliで発現させ、活性等の諸性質について調べている。

close this sectionSequence

selected fasta
>putative glucose-1-phosphate thymidylyltransferase [putative dTDP-glucose synthase]
MKGIILAGGTGSRLHPTTLAVSKQLLPVGDKPMIYYPLSVLMLAGVTDILIISTPHELPR
MRRLFGDGAQLGLRLAYAEQEKPRGIAEAFLIGADHVGSDAVALALGDNIFHGSSFQGVL
RKEAEELDGCVLFGYPVKDPQRYGVGEANASGRLVSIEEKPVRPRSNRAITGLYFYDNEV
VDIARRLRPSARGELEITDINRTYMERGRARLVDLGRGFAWLDTGTPESLLQASQYVSAL
EERQGIRIACIEEVALRMGFINAQACYELGARLSGSGYGQYVMAIAEECTGRV
selected fasta
>putative glucose-1-phosphate thymidylyltransferase [putative dTDP-glucose synthase]
GTGAAGGGAATCATTCTCGCCGGGGGTACGGGGAGCAGGCTCCACCCGACGACTCTCGCG
GTGTCCAAGCAGCTTCTCCCCGTCGGGGACAAGCCGATGATCTACTACCCGCTCTCCGTG
CTGATGCTGGCCGGCGTCACGGACATCCTCATCATCAGCACACCGCACGAACTCCCCCGA
ATGCGCCGTCTGTTCGGCGACGGCGCACAGCTCGGACTCCGCCTGGCCTACGCCGAGCAG
GAGAAACCCAGGGGTATCGCCGAGGCGTTCCTGATCGGTGCCGACCACGTGGGAAGCGAT
GCCGTTGCGCTGGCGCTGGGCGACAACATATTCCACGGGAGTTCTTTTCAGGGGGTGCTG
CGCAAGGAAGCCGAGGAATTGGACGGGTGTGTCCTGTTCGGTTATCCGGTCAAGGATCCC
CAGCGTTATGGAGTCGGCGAGGCGAACGCGTCCGGGCGGCTCGTCTCCATCGAGGAGAAA
CCGGTACGCCCCCGCTCCAACCGGGCCATCACCGGACTCTATTTCTACGACAACGAGGTG
GTGGACATCGCCCGGCGGCTGCGCCCCTCCGCCCGCGGCGAACTCGAAATCACCGACATC
AACCGTACCTACATGGAACGAGGCCGGGCCCGGCTCGTGGACCTGGGCCGGGGATTCGCC
TGGCTCGACACCGGCACACCCGAGTCACTCCTGCAGGCCTCGCAGTACGTGTCCGCCCTG
GAGGAACGCCAGGGCATCAGGATCGCCTGCATCGAGGAGGTGGCCCTCCGCATGGGCTTC
ATCAACGCCCAGGCCTGCTACGAACTGGGCGCGCGCCTGTCCGGCTCCGGCTACGGGCAG
TACGTGATGGCCATCGCGGAGGAGTGCACGGGGCGGGTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005835 Nucleotidyl transferase (Domain)
[2-237] 8.20000000000006e-59 PF00483
PF00483   NTP_transferase
IPR005907 Glucose-1-phosphate thymidylyltransferase, short form (Family)
[2-286] 1.99999999999999e-125 TIGR01207
TIGR01207   RmlA
SignalP No significant hit
TMHMM No significant hit
Nogl_00260 Nogl_00260   Nogl_00280 Nogl_00280
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