Nogl_00280 : CDS information

Location

Organism	Streptomyces nogalater
Strain	ATCC 27451 (=NBRC 13445)
Entry name	Nogalamycin
Contig	AF187532
Start / Stop / Direction	27,676 / 28,701 / + [in whole cluster] 15,039 / 16,064 / + [in contig]
Location	27676..28701 [in whole cluster] 15039..16064 [in contig]
Type	CDS
Length	1,026 bp (341 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-3 aminotransferase(fragment)
Product (GenBank)	putative aminotransferase
Gene
Gene (GenBank)	snogI
EC number
Keyword	nogalamine
Note
Note (GenBank)
Reference	ACC Q9RN51 PmId [11683270] The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures. (Mol Genet Genomics. , 2001) comment S. nogalater genomic DNA fragmentの配列解析と、異種性発現による産物解析。 nogalamycin生合成に関する20ORFsが明らかになった。 snogI: aminotransferase 配列類似性からnogalamine生合成に必要なaminotransferaseとして同定されている。
Related Reference	ACC Q9F837 NITE Megalo_00050 PmId [16514147] In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea. (Microbiology. , 2006) [20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010) comment Blast 19th, id71%, 1e-140 Micromonospora megalomicea subsp. nigra_megDII TDP-3-keto-6-deoxyhexose 3-aminotransaminase [DoBISCUIT]NDP-hexose C-3 aminotransferase 異なる遺伝子セットを含んだオペロンによってE.coliで産生されたdTDP-sugar中間体のLC/MS/MS解析。セットの組み合わせによりMegDII の機能は確立されている。 MegDII は、desosamineとmegosamine両方の生合成において3-aminotransferaseとして働く。基質は各経路で少し異なる。

Sequence

>putative NDP-hexose C-3 aminotransferase(fragment) [putative aminotransferase]
MTVHVWDYLPEYELEREDIHDAVETVFRSGRLVLGESVRGFESEFASFQGVGHAVGVDNG
TNAVKLGLQALGVGPGDEVVTVSNTAAPTVVAIDSAGATPVFVDVREEDYLMDTSQVEAV
LTPRTRCLLPVHLYGQCVDMAPLRDLAARHNLVILEDCAQAHGARRHGRLAGSTGDAAAF
SFYPTKVLGAYGDGGAVLTDDERVADRLRRLRYYGMESRYYVVETPGHNSRLDEVQAEIL
RRKLSRLPSYIEARRAVARRYEEGLADTGLLLPRTAQGNEHVYYVYVVRHPRRDAVLEAL
RASYDIALNISYPWPVHTMTGFSHLGYAKGSLPVTEALADEI

>putative NDP-hexose C-3 aminotransferase(fragment) [putative aminotransferase]
ATGACCGTACACGTGTGGGACTATCTGCCGGAATACGAGCTGGAACGCGAGGACATCCAC
GATGCCGTGGAGACGGTGTTCCGCTCCGGCCGGCTCGTCCTCGGTGAGAGCGTGCGCGGC
TTCGAGTCCGAGTTCGCCTCATTCCAGGGCGTCGGACATGCCGTCGGCGTCGACAACGGG
ACCAACGCCGTCAAGCTGGGGTTACAGGCCTTGGGTGTCGGGCCCGGCGACGAGGTCGTC
ACCGTGTCCAACACCGCCGCGCCCACGGTCGTCGCGATCGACTCGGCCGGTGCCACGCCC
GTCTTCGTGGACGTGCGCGAGGAGGACTACCTGATGGACACGTCCCAGGTGGAGGCCGTC
CTCACGCCACGCACCCGGTGTCTGCTGCCGGTCCACCTCTACGGGCAGTGCGTGGACATG
GCACCGCTGCGCGATCTGGCCGCCCGGCACAACCTGGTGATCCTGGAGGACTGCGCGCAG
GCGCACGGGGCGCGCCGGCACGGCCGACTCGCCGGCTCGACCGGTGACGCGGCCGCGTTC
TCCTTCTACCCGACCAAGGTGCTGGGCGCGTACGGGGACGGCGGCGCGGTGCTCACCGAC
GACGAGCGGGTGGCGGACCGGCTGCGGCGGCTGCGCTACTACGGCATGGAGAGCCGCTAC
TACGTCGTCGAAACCCCCGGGCACAACAGCCGGCTGGACGAAGTACAGGCAGAGATCCTG
CGGCGCAAGCTGTCCCGGTTGCCGTCCTACATCGAGGCCCGCCGGGCGGTGGCACGCCGC
TATGAGGAGGGCCTGGCGGACACGGGGCTGCTGCTGCCGAGGACCGCGCAGGGCAACGAG
CACGTCTACTACGTGTACGTGGTGCGCCATCCGCGACGGGACGCGGTGCTGGAGGCCCTG
CGCGCCTCCTACGACATCGCCCTCAACATCAGTTATCCCTGGCCCGTGCACACCATGACC
GGTTTCTCCCATCTCGGCTACGCCAAGGGCTCGTTGCCGGTCACCGAGGCACTGGCGGAC
GAGATC

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family) PIRSF000390 PLP_StrS PF01041 DegT_DnrJ_EryC1 IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain) G3DSA:3.40.640.10 PyrdxlP-dep_Trfase_major_sub1 IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain) G3DSA:3.90.1150.10 PyrdxlP-dep_Trfase_major_sub2 IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain) SSF53383 PyrdxlP-dep_Trfase_major
SignalP	No significant hit
TMHMM	No significant hit

Nogl_00270 Nogl_00290