Nogl_00290 : CDS information

Location

Organism	Streptomyces nogalater
Strain	ATCC 27451 (=NBRC 13445)
Entry name	Nogalamycin
Contig	AJ224512
Start / Stop / Direction	30,320 / 28,854 / - [in whole cluster] 1,618 / 152 / - [in contig]
Location	complement(28854..30320) [in whole cluster] complement(152..1618) [in contig]
Type	CDS
Length	1,467 bp (488 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	SnogH
Gene
Gene (GenBank)	snogH
EC number
Keyword	nogalamine
Note
Note (GenBank)
Reference	ACC O54255 PmId [9349712] Characterization of Streptomyces nogalater genes encoding enzymes involved in glycosylation steps in nogalamycin biosynthesis. (Mol Gen Genet. , 1997) comment snoF, snoG, snoHが同定されている論文。 snoHの推定産物は489aa polypeptideである。 daunomycin clusterのdau-ORF3の産物に似ている。しかしその機能はまだ明らかでない。相補実験と配列解析から、snoFGHはnogalamycinの糖部分の生合成に関連すると示唆される。また、sno minimal PKSのtranscriptional activator SnoAがこのclusterを発現するために必要。
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment Blast 19th, id53%, 1e-125 Saccharopolyspora spinosa_spnO Probable NDP-hexose-2,3-dehydratase SpnOはforosamine合成の初期段階でNDP-4-keto-6-deoxy-glucoseの2,3位の脱水反応を行う。 dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltol に分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [SnogH]
MTKLSAHPAAPAHGAVPDPLRLAASARSAGVWLPRTTEVHHWLATVGQHARAQVERVPLA
ELDGWLRDPATGNIAHRSGGFFTVEGLDVTIPRAPVPHWEQPIINQPEVGLLGIVVKEFD
GVLHCLMQAKLEPGNCNGIQLSPTVQATRSNYTRVHRGRPVPYLEHFQDPARRSRILADV
RQSEQGSWFHHKRNRNMVVEVEEDIDVHDGFCWLSLGQLHELLTVDNLINMDTRTVLSCL
PFAHAGLAAVAAPGADPFRRALVASCAPDGYSRHSLGDLLSWITDVRTRTEVRTEMVPLD
GLRSWRRTDERISHEDGGFFDVIGVRVRTRGREVAEWTQPMIEPHAKGVVAFLVRPIEGV
LHVLVHARVEPGYVDIVELAPTVQCTPDSYERLPARARPLFLDEVLPARADRVRFDAELS
EEGGRFYHARNRYLVAETDLAAGFDHPDFRWVTLAQLVELLRHSHYVNIQARSLVACLYG
LATAPPRR

>putative NDP-hexose 2,3-dehydratase [SnogH]
ATGACGAAACTGTCCGCCCACCCGGCCGCCCCCGCGCACGGTGCCGTGCCGGACCCGCTG
CGGCTGGCTGCCTCGGCCCGCTCGGCGGGTGTGTGGCTGCCACGGACCACCGAGGTCCAC
CACTGGCTGGCCACGGTGGGGCAACACGCCCGGGCCCAGGTCGAGCGGGTCCCCCTGGCC
GAGCTCGACGGCTGGCTCCGCGACCCGGCGACCGGCAACATCGCCCACCGCAGCGGGGGC
TTCTTCACCGTGGAGGGGCTCGACGTGACCATCCCCCGGGCGCCCGTGCCGCACTGGGAG
CAGCCGATCATCAACCAGCCCGAGGTCGGGCTCCTCGGCATCGTCGTCAAGGAGTTCGAC
GGCGTCCTGCACTGCCTCATGCAGGCCAAGCTGGAACCCGGCAACTGCAACGGCATCCAG
CTGTCGCCTACCGTGCAGGCCACCCGCAGCAACTACACCCGGGTGCACCGCGGCCGGCCG
GTGCCGTATCTGGAGCACTTCCAGGACCCCGCCCGCCGGAGCCGGATCCTGGCCGACGTG
CGCCAGTCCGAGCAGGGCTCCTGGTTCCACCACAAACGCAACCGCAACATGGTCGTCGAG
GTCGAGGAGGACATCGACGTCCACGACGGCTTCTGCTGGCTGTCGCTGGGCCAGCTCCAC
GAGCTGCTGACCGTGGACAACCTGATCAACATGGACACCCGTACGGTGCTGTCCTGTCTG
CCCTTCGCCCACGCCGGCCTCGCGGCGGTGGCCGCCCCCGGCGCCGACCCGTTCCGCCGC
GCGCTGGTCGCCTCCTGCGCTCCCGACGGCTACAGCCGGCACTCGCTGGGCGACCTGCTG
AGCTGGATCACGGACGTGCGTACGCGCACCGAGGTGCGCACGGAGATGGTGCCGCTCGAC
GGCCTGCGGTCCTGGCGTCGCACGGACGAGCGGATCAGCCACGAGGACGGTGGCTTCTTC
GATGTGATCGGCGTGCGGGTGCGGACGCGGGGGCGCGAGGTCGCCGAGTGGACCCAGCCG
ATGATCGAACCGCACGCCAAGGGCGTCGTGGCCTTCCTCGTCCGGCCCATCGAGGGCGTG
CTGCACGTCCTGGTCCACGCGCGCGTGGAGCCCGGGTACGTCGACATCGTCGAGCTGGCG
CCGACCGTGCAGTGCACACCCGACAGCTACGAACGGCTGCCGGCGCGGGCCCGCCCGCTC
TTCCTCGACGAGGTTCTGCCGGCCCGGGCCGACCGAGTCCGCTTCGACGCCGAGCTCTCC
GAGGAGGGCGGCCGCTTCTACCACGCGCGCAACCGCTACCTGGTGGCGGAGACCGACCTG
GCCGCCGGCTTCGACCACCCCGACTTCCGCTGGGTGACGCTGGCCCAACTCGTGGAGCTG
CTGCGGCACAGTCACTACGTCAACATCCAGGCGCGCAGTCTCGTCGCCTGCCTGTACGGC
CTCGCCACGGCGCCGCCGAGGCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	No significant hit
TMHMM	No significant hit

Nogl_00280 Nogl_00300