Ovied_00010 : CDS information

Location

Organism	Streptomyces antibioticus
Strain	ATCC 11891
Entry name	Oviedomycin
Contig	AJ632203
Start / Stop / Direction	2 / 1,219 / + [in whole cluster] 2 / 1,219 / + [in contig]
Location	2..1219 [in whole cluster] 2..1219 [in contig]
Type	CDS
Length	1,218 bp (405 aa)

Annotation

Category	5.1 general function
Product	putative glutamate--tRNA ligase(fragment) putative glutamyl-tRNA synthetase(fragment)
Product (GenBank)	tRNA-synthetase class I
Gene	gltX
Gene (GenBank)
EC number	6.1.1.17
Keyword
Note	It is supposed that this ORF is not involved in oviedomycin biosynthesis.
Note (GenBank)	ORF1
Reference	ACC Q6ZZX2 PmId [15368568] Genetic organization of the biosynthetic gene cluster for the antitumor angucycline oviedomycin in Streptomyces antibioticus ATCC 11891. (Chembiochem. , 2004) comment oviedomycin生合成gene clusterの報告。 ORF1(incomplete): truncated Glu-tRNA-synthetase このORFは、おそらくoviedomycin生合成に関連しないと述べられている。
Related Reference	ACC P04805 PmId [3015933] ( , ) comment Blast 420th, id35%, 8e-54 E.coli_gltX Glutamate--tRNA ligase(EC 6.1.1.17) synonym: Glutamyl-tRNA synthetase 機能解析、配列決定。
Family/Domain	FD IPR004527 comment [IPR004527] Glutamyl-tRNA synthetase, class Ic, bacterial/mitochondrial (Family) function; glutamate-tRNA ligase activity, nucleotide binding, ATP binding

Sequence

>putative glutamate--tRNA ligase(fragment) [tRNA-synthetase class I]
DPAFEGPYFQSHNAGRHREAAEGLFAAGRAYYCDCTREQLKERTGSEHLGYDGFCRERGL
GYAAGRALRFRTPDEGETVVADLVRGEPAFPNSAIEDFVIARGDGSPVFLLANVVDDLDE
GITQVIRGEEHLSNTPKQQLLWEALGAGPPVWAHLPVIVNEKRQKLSKRRDKVALEDYLG
EGFLPEALVNYLMLLGWGPGGDREIMPYEELERLFRIEDVNTAPAFFDVKKLTAFNGEYL
RALAPAQFAAACVPWLVAPYAPWRPEAFDKDVFEAAAPLARTRLTRLSEITGYVDFLFLD
EPVDDEVSWHKAMKEGAADILTDVRAGLAAVTGWTAEELKGVLLAVGEQHGLKLGKAQAP
VRVAVTGRTVGLPLFRVPGTGSAGSGCWPGWTPQIRRLAGRGAPG

>putative glutamate--tRNA ligase(fragment) [tRNA-synthetase class I]
GATCCGGCTTTCGAGGGGCCGTACTTCCAGTCGCACAACGCCGGCCGGCACCGGGAGGCG
GCCGAGGGGCTGTTCGCGGCGGGGCGGGCGTACTACTGCGACTGCACCCGGGAGCAGCTG
AAGGAGCGCACGGGGTCGGAGCACCTGGGGTACGACGGGTTCTGCCGGGAGCGGGGGCTC
GGGTACGCGGCCGGGCGGGCGCTGCGGTTCCGGACGCCGGACGAGGGGGAGACCGTCGTC
GCCGACCTGGTGCGCGGCGAGCCGGCGTTCCCGAACAGCGCGATCGAGGACTTCGTGATC
GCCCGCGGTGACGGCTCTCCGGTCTTCCTGCTCGCCAACGTCGTGGACGACCTGGACGAG
GGCATCACGCAGGTCATCCGGGGCGAGGAGCATCTGTCGAACACACCGAAGCAGCAGCTG
CTGTGGGAGGCGCTCGGTGCCGGGCCGCCGGTGTGGGCGCATCTGCCGGTCATCGTGAAC
GAGAAGCGGCAGAAGCTGTCCAAGCGCCGGGACAAGGTCGCGCTGGAGGACTACCTCGGC
GAGGGCTTCCTGCCCGAGGCGCTGGTGAACTACCTGATGCTGCTCGGCTGGGGCCCGGGC
GGCGACCGGGAGATCATGCCGTACGAGGAGCTGGAGCGGCTCTTCCGGATCGAGGACGTC
AACACCGCCCCGGCCTTCTTCGACGTGAAGAAGCTGACGGCCTTCAACGGCGAGTACCTC
CGCGCCCTGGCGCCCGCGCAGTTCGCCGCGGCCTGTGTGCCGTGGCTCGTCGCGCCGTAC
GCCCCGTGGCGGCCGGAGGCGTTCGACAAGGACGTCTTCGAGGCGGCGGCTCCGCTGGCC
CGGACCCGGTTGACGCGGCTCTCCGAGATCACCGGCTACGTGGACTTCCTGTTCCTCGAT
GAGCCGGTGGACGACGAGGTGTCGTGGCACAAGGCGATGAAGGAGGGGGCCGCGGACATC
CTCACGGACGTCCGCGCCGGACTGGCCGCCGTCACCGGCTGGACGGCGGAGGAGCTGAAG
GGCGTCCTGCTCGCCGTGGGGGAGCAGCACGGGCTGAAGCTGGGCAAGGCCCAGGCCCCG
GTCCGGGTCGCGGTCACCGGGCGCACGGTCGGGCTCCCGCTGTTTCGAGTCCCTGGAACT
GGCTCGGCCGGGAGCGGGTGCTGGCCCGGCTGGACACCGCAGATCCGGAGACTGGCGGGG
CGGGGCGCGCCGGGGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004527 Glutamyl-tRNA synthetase, class Ib, bacterial/mitochondrial (Family) TIGR00464 GltX_bact IPR008925 Aminoacyl-tRNA synthetase, class I, anticodon-binding (Domain) SSF48163 tRNA-synt_bind IPR014729 Rossmann-like alpha/beta/alpha sandwich fold (Domain) G3DSA:3.40.50.620 Rossmann-like_a/b/a_fold IPR020058 Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain (Domain) PF00749 tRNA-synt_1c IPR020061 Glutamyl/glutaminyl-tRNA synthetase, class Ib, alpha-bundle domain (Domain) G3DSA:1.10.1160.10 Glu/Gln-tRNA-synth_Ic_a-bdl IPR020751 Aminoacyl-tRNA synthetase, class I, anticodon-binding domain, subdomain 2 (Domain) G3DSA:1.10.10.350 tRNA_synt_bd
SignalP	No significant hit
TMHMM	No significant hit

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