Ovied_00110 Ovied_00110   Ovied_00130 Ovied_00130

Ovied_00120 : CDS information

close this sectionLocation

Organism
StrainATCC 11891
Entry nameOviedomycin
Contig
Start / Stop / Direction11,006 / 12,229 / + [in whole cluster]
11,006 / 12,229 / + [in contig]
Location11006..12229 [in whole cluster]
11006..12229 [in contig]
TypeCDS
Length1,224 bp (407 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase chain length factor subunit
Product (GenBank)keto-acyl synthase beta
Gene
Gene (GenBank)ovmK
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[15368568] Genetic organization of the biosynthetic gene cluster for the antitumor angucycline oviedomycin in Streptomyces antibioticus ATCC 11891. (Chembiochem. , 2004)
comment
oviedomycin生合成gene clusterの報告。

OvmK: ketoacyl synthase beta

特徴的なCys174 and Gln166を保存している。
ovmP, ovmK, ovmSはtype II minimal PKSをコードする。
starter acetyl-CoAとmalonyl-CoA 9つからdecaketideを合成する。

ovmK deletion mutantはoviedomycin非産生。
このmutantにおいて気菌糸、胞子形成には影響がなかった。
よってS. antibioticusにおけるoviedomycin産生と細胞分化の間の直接的関係が除外された。
Related Reference
ACC
Q56164
NITE
Jado_00090
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 7th, id79%, 1e-175
Streptomyces venezuelae_jadB
Chain length determinant

---
[PMID:7881555](1994)
ORF2(=JadBに相当)は配列解析からtype II PKSのCLFであると提唱されている。
ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
jadBもKS-betaとして使用されている。

close this sectionPKS/NRPS Module

CLF6..365

close this sectionSequence

selected fasta
>polyketide synthase chain length factor subunit [keto-acyl synthase beta]
MTGTATKVVVTGLGVAAPNGLGTQDYWAATRTGKGGIGRITRFDPSQYPSRLAGEVPGFE
ARDHLPSRLLPQTDHMTRLALVAADWALADAGVKPQELPEFDMGVVTASSSGGFEFGQRE
LQALWSKGGQHVSAYQSFAWFYAVNSGQISIRNGMKGPSGVVVSEQAGGLDALAQARRQI
RKGTRLIVSGGMDASVCTWGWVAQLAGKRLSTSDDPERAYLPFDAGARGHVPGEGGAILI
LENAEAARSRGARTYGEIAGYGSTFDPKPGSGRPPGLRKAIELALADAGAAPDEVDVVFA
DAAAVPELDRIEAEALTAVFGARGVPVTAPKTMTGRLYSGAAPLDVATALLAIEEGLIPP
TTNVEPADGYDLDLVTGEARPAEVRTALVLARGFNGFNSALVVRATH
selected fasta
>polyketide synthase chain length factor subunit [keto-acyl synthase beta]
ATGACCGGCACGGCCACGAAGGTGGTGGTCACCGGACTGGGCGTCGCGGCCCCCAACGGC
CTGGGCACCCAGGACTACTGGGCGGCGACCCGGACCGGCAAGGGCGGCATCGGCCGCATC
ACCCGCTTCGACCCCTCGCAGTACCCCTCCCGGCTGGCCGGCGAGGTCCCCGGCTTCGAG
GCCCGCGACCACCTGCCCAGCCGGCTGCTGCCGCAGACCGACCACATGACGCGGCTCGCG
CTCGTGGCCGCCGACTGGGCGCTCGCCGACGCCGGGGTCAAGCCGCAGGAGCTGCCCGAG
TTCGACATGGGCGTGGTCACCGCCAGCTCCTCGGGCGGCTTCGAGTTCGGCCAGCGCGAA
CTCCAGGCGCTGTGGAGCAAGGGCGGCCAGCACGTCAGCGCCTACCAGTCCTTCGCCTGG
TTCTACGCGGTCAACTCCGGCCAGATTTCGATTCGCAACGGCATGAAGGGGCCCAGCGGC
GTCGTCGTCAGCGAGCAGGCGGGCGGCCTCGACGCGCTCGCCCAGGCCCGCCGGCAGATC
CGCAAGGGCACCCGGCTGATCGTCTCCGGCGGCATGGACGCGTCCGTGTGCACCTGGGGG
TGGGTCGCCCAGCTGGCCGGCAAGCGGCTGAGCACCAGCGACGACCCCGAGCGCGCCTAC
CTGCCGTTCGACGCCGGGGCGCGCGGGCACGTCCCGGGCGAGGGCGGCGCGATCCTCATC
CTGGAGAACGCCGAGGCCGCCCGGTCCCGGGGCGCCCGGACCTACGGCGAGATCGCGGGC
TACGGCTCCACGTTCGACCCCAAGCCGGGCAGCGGCCGCCCGCCGGGCCTGCGCAAGGCG
ATCGAGCTGGCCCTCGCGGACGCGGGCGCGGCCCCGGACGAGGTGGACGTGGTGTTCGCC
GACGCCGCCGCCGTCCCGGAGCTGGACCGCATCGAGGCCGAGGCGCTCACCGCCGTCTTC
GGCGCCCGCGGCGTCCCGGTCACCGCGCCCAAGACCATGACCGGCCGCCTGTACTCCGGC
GCCGCCCCGCTGGACGTGGCCACCGCGCTGCTCGCCATCGAGGAGGGGCTGATCCCGCCC
ACCACGAACGTCGAGCCGGCCGACGGCTACGACCTGGACCTGGTCACCGGCGAGGCCCGC
CCGGCCGAGGTGCGCACGGCGCTCGTGCTGGCCCGCGGCTTCAACGGGTTCAACTCCGCG
CTCGTGGTCCGCGCCACCCACTGA
CLF6..365
CLF16..1095

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[6-246] 1e-57 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[255-365] 1.1e-27 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[6-260] 1.6e-55 G3DSA:3.40.47.10 [272-405] 1.5e-39 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[5-252] 2.60000517657733e-63 SSF53901 [215-406] 6.70002630158883e-49 SSF53901
SSF53901   Thiolase-like
SignalP
[1-22] 0.584 Signal
Eukaryota   
[1-28] 0.305 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Ovied_00110 Ovied_00110   Ovied_00130 Ovied_00130
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