Ovied_00120 Ovied_00120   Ovied_00140 Ovied_00140

Ovied_00130 : CDS information

close this sectionLocation

Organism
StrainATCC 11891
Entry nameOviedomycin
Contig
Start / Stop / Direction12,310 / 12,582 / + [in whole cluster]
12,310 / 12,582 / + [in contig]
Location12310..12582 [in whole cluster]
12310..12582 [in contig]
TypeCDS
Length273 bp (90 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)acyl carrier protein
Gene
Gene (GenBank)ovmS
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[15368568] Genetic organization of the biosynthetic gene cluster for the antitumor angucycline oviedomycin in Streptomyces antibioticus ATCC 11891. (Chembiochem. , 2004)
comment
oviedomycin生合成gene clusterの報告。

OvmS: acyl carrier protein

配列解析のみ。
4'-phosphopantetheine attachment siteに関連したSer144保存あり。
ovmP, ovmK, ovmSはtype II minimal PKSをコードする。
starter acetyl-CoAとmalonyl-CoA 9つからdecaketideを合成する。
Related Reference
ACC
Q56165
NITE
Jado_00100
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 3rd, id72%, 8e-27
Streptomyces venezuelae_acp(jadC)
Acyl carrier protein

--
[PMID:7881555](1994)
ORF3(=JadC)は配列解析からtype II PKSのACPであると提唱されている。
ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
jadCもACPとして使用されている。

close this sectionPKS/NRPS Module

ACP7..81

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
MATKEFTVDDLKRILLEGAGADESVDLDSADFLDSEFEALGYESLALLETGGRIEREFGI
TLDDTALTDATTPRALIEVVNAHIGQPTAV
selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
ATGGCCACCAAGGAATTCACCGTCGACGACCTCAAGCGCATCCTCCTCGAAGGCGCCGGC
GCCGACGAGAGCGTCGACCTCGACAGCGCCGACTTCCTCGACTCCGAGTTCGAGGCGCTG
GGCTACGAGTCCCTGGCGCTGCTGGAGACCGGCGGCCGCATCGAGCGCGAGTTCGGCATC
ACGCTGGACGACACCGCGCTGACCGACGCCACCACCCCGCGTGCCCTCATCGAGGTCGTC
AACGCGCACATCGGTCAGCCCACCGCGGTCTGA
ACP7..81
ACP19..243

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006162 Phosphopantetheine attachment site (PTM)
[39-54] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[4-89] 2.79999889404114e-12 SSF47336
SSF47336   ACP_like
[11-79] 4.3e-09 PF00550
PF00550   PP-binding
[4-86] 2.5e-17 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[7-81] PS50075
PS50075   ACP_DOMAIN
SignalP
[1-21] 0.051 Signal
Eukaryota   
TMHMM No significant hit
Ovied_00120 Ovied_00120   Ovied_00140 Ovied_00140
Page top