Ovied_00130 Ovied_00130   Ovied_00150 Ovied_00150

Ovied_00140 : CDS information

close this sectionLocation

Organism
StrainATCC 11891
Entry nameOviedomycin
Contig
Start / Stop / Direction12,706 / 13,488 / + [in whole cluster]
12,706 / 13,488 / + [in contig]
Location12706..13488 [in whole cluster]
12706..13488 [in contig]
TypeCDS
Length783 bp (260 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative polyketide C-9 ketoreductase
Product (GenBank)ketoreductase
Gene
Gene (GenBank)ovmT
EC number
Keyword
Note
Note (GenBank)
  • actIII homologue
Reference
ACC
PmId
[15368568] Genetic organization of the biosynthetic gene cluster for the antitumor angucycline oviedomycin in Streptomyces antibioticus ATCC 11891. (Chembiochem. , 2004)
comment
oviedomycin生合成gene clusterの報告。

OvmT: C9-ketoreductase

配列解析のみ。
short-chain alcohol dehydrogenase domainを含む。
たぶん新生polyketide鎖のC9 keto基をOH基に還元する。
これは続いて起こるaromatizationのために必要な修飾である。
Related Reference
ACC
Q56166
NITE
Jado_00110
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 2nd, id90%, 1e-130
Streptomyces venezuelae_ORF5(jadE)
Ketoreductase

--
[PMID:7881555](1994)
ORF5(=jadEに相当)は配列解析からketoreductaseであると提唱されている。
ORF2-ORF4領域(ORF5も含まれる)を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、
そのplasmid発現株での産物を解析。
JadEはdecaketideだけでなく、octaketideも還元できる。

close this sectionSequence

selected fasta
>putative polyketide C-9 ketoreductase [ketoreductase]
MAQDKRVALVTGATSGIGLAVARLLAEQGHRVFLGARSAENVAATVKELQSAGLEADGAT
LDVRSDADVHAFVRAAVDRFGTVDVLVNNAGRSGGGVTADIADELWHDVIDTNLNSVFRL
TREVLNTGGLRHKSWGRIINIASTAGKQGVVLGAPYSASKHGVVGFTKALGNELAPTGIT
VNAVCPGYVETPMAQRVRQGYAAAYDTTEDAILEKFQSKIPLGRYSTPEEVAGLVGYLAS
DTAASITAQALNVCGGLGNF
selected fasta
>putative polyketide C-9 ketoreductase [ketoreductase]
ATGGCGCAGGACAAGCGGGTCGCCCTGGTGACCGGGGCGACCAGCGGCATCGGTCTGGCC
GTGGCCCGGCTGCTGGCCGAGCAGGGGCACCGGGTGTTCCTGGGCGCGCGCAGCGCCGAG
AACGTGGCCGCGACCGTGAAGGAACTGCAGAGCGCCGGCCTGGAGGCGGACGGCGCCACG
CTGGACGTCCGCTCCGACGCCGACGTCCACGCCTTCGTGCGGGCGGCCGTGGACCGGTTC
GGCACGGTGGACGTGCTGGTGAACAACGCCGGCCGCAGCGGTGGCGGAGTGACCGCGGAC
ATCGCCGACGAGCTGTGGCACGACGTGATCGACACGAACCTCAACAGCGTGTTCCGGCTG
ACCCGCGAGGTGCTGAACACCGGTGGCCTCAGGCACAAGAGCTGGGGCCGGATCATCAAC
ATCGCCTCCACCGCGGGCAAGCAGGGCGTCGTCCTGGGCGCCCCGTACTCGGCGTCCAAG
CACGGCGTGGTCGGCTTCACCAAGGCCCTCGGCAACGAGCTGGCCCCGACCGGGATCACC
GTCAACGCGGTCTGCCCGGGCTACGTCGAGACGCCGATGGCCCAGCGGGTGCGCCAGGGC
TACGCCGCCGCCTACGACACCACCGAGGACGCGATCCTGGAGAAGTTCCAGTCCAAGATC
CCGCTCGGCCGCTACTCCACCCCCGAGGAGGTCGCCGGGCTGGTCGGCTACCTGGCCTCC
GACACCGCCGCCTCCATCACCGCCCAGGCGCTCAACGTCTGCGGCGGCCTCGGCAACTTC
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[81-92] 5.79999653854781e-15 PR00080 [136-144] 5.79999653854781e-15 PR00080 [156-175] 5.79999653854781e-15 PR00080
PR00080   SDRFAMILY
[1-260] 5.20000089003336e-16 PIRSF000126
PIRSF000126   11-beta-HSD1
[7-174] 5.30000000000001e-36 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[7-24] 7.90003079443025e-50 PR00081 [81-92] 7.90003079443025e-50 PR00081 [130-146] 7.90003079443025e-50 PR00081 [156-175] 7.90003079443025e-50 PR00081 [177-194] 7.90003079443025e-50 PR00081 [221-241] 7.90003079443025e-50 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[4-257] 4.49999999999996e-83 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[143-171] PS00061
PS00061   ADH_SHORT
SignalP
[1-26] 0.806 Signal
Eukaryota   
[1-26] 0.925 Signal
Bacteria, Gram-positive   
[1-22] 0.819 Signal
Bacteria, Gram-negative   
TMHMM
[7-26] Transmembrane (i-o)
Transmembrane 1   
Ovied_00130 Ovied_00130   Ovied_00150 Ovied_00150
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