Ovied_00170 : CDS information

Location

Organism	Streptomyces antibioticus
Strain	ATCC 11891
Entry name	Oviedomycin
Contig	AJ632203
Start / Stop / Direction	15,945 / 17,465 / + [in whole cluster] 15,945 / 17,465 / + [in contig]
Location	15945..17465 [in whole cluster] 15945..17465 [in contig]
Type	CDS
Length	1,521 bp (506 aa)

Annotation

Category	3.1 modification hydroxylation
Product	hydroxylase
Product (GenBank)	oxygenase
Gene
Gene (GenBank)	ovmOIII
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q6ZZV6 PmId [15368568] Genetic organization of the biosynthetic gene cluster for the antitumor angucycline oviedomycin in Streptomyces antibioticus ATCC 11891. (Chembiochem. , 2004) [18988223] Elucidation of oxygenation steps during oviedomycin biosynthesis and generation of derivatives with increased antitumor activity. (Chembiochem. , 2009) comment [PMID:15368568](2004) OvmOIII: oxygenase oviedomycin生合成gene clusterの報告。 ovmOIII については配列解析のみ。 -- [PMID:18988223](2009) FAD-dependent oxygenase genes (ovmOI, ovmOII, and ovmOIII)の解析。 PKS、cyclase, aromatase, and reductase genesと共にこれらFAD-dependent oxygenasesの異なる組み合わせを持ったplasmidsを構築して産物解析。 ovmOIII 以外のoviedomycin生合成必須遺伝子を組み込まれたS.albus株は、5-hydroxy-dehydrorabelomycinを蓄積。(C-5 hydroxylationはOvmOI またはhost geneによる) ovmOII 以外のoviedomycin生合成必須遺伝子を組み込まれたS.albus株は、rabelomycinと9-hydroxyrabelomycinを蓄積。これらはOvmOIII があるのにC-2 OH基を持たない。この結果からOvmIII は、aromatic ringAでないとC-2 hydroxylationできず、その代りにC-9をhydroxylateすると述べている。

Sequence

>hydroxylase [oxygenase]
MSKSAPRPPAGPRTATGPEVLVVGAGPTGLLLAGDLAAAGTRVTVLEKRGRKLSNLTRSF
TVHARTLEQLQARGVAEDIIATGTKLPTLPLFGDLDIHFAHLRTRYPYVLITPQFHVEQV
LERRAVSLGVEFRHDTEVTGLRQDAHGVELDAVTADGTRETHRAAYAVGTDGVRSTVRRL
LDIPFPGDSVLRSVILADVLMKEAPADLLTVDANEHGFAFVAPFGDGYYRVLAWNRHTDS
SADESVDFEELRRLCRAILGRDFGMHEPRWVSRFHSDERQATRYREGRFFLAGDAAHVHS
PAGGMGMNTGLQDAMNLAWKLAAAVRGHGDDALLDTYEAETHPVGEEVLRTSGSLVRAAI
RQSGRTRLAQQAMVWFAGHLGPLGEALGRREGLAVSGTGITYGRQRGDHRLVGRFFHGRL
TGGRLDGALGTGRFVLLSPDEAVRTAVADWSDRVVTAPADVRTTLLIRPDGYVAWAADGP
GPAAVRDALNRWAGPAEPRIRLAYAG

>hydroxylase [oxygenase]
ATGTCGAAGTCCGCACCGCGGCCCCCGGCGGGCCCCCGCACGGCGACCGGCCCCGAGGTC
CTGGTCGTCGGGGCGGGCCCCACCGGCCTGCTGCTGGCGGGAGACCTCGCCGCCGCCGGC
ACCCGGGTCACCGTCCTGGAGAAGCGCGGCCGGAAACTGAGCAACCTGACCCGCTCGTTC
ACCGTGCACGCGCGCACCCTGGAGCAGCTTCAGGCGCGCGGCGTCGCCGAGGACATCATC
GCCACCGGCACCAAGCTGCCCACCCTGCCGCTCTTCGGCGACCTGGACATCCACTTCGCG
CACCTGCGCACCCGCTACCCGTACGTGCTGATCACCCCGCAGTTTCACGTGGAACAAGTG
CTGGAGCGACGGGCCGTCTCCCTCGGGGTGGAGTTCCGCCACGACACCGAGGTGACCGGG
CTGCGCCAGGACGCGCACGGGGTCGAGCTGGACGCCGTCACCGCCGACGGCACCCGGGAG
ACCCACCGTGCGGCCTACGCGGTCGGCACCGACGGGGTGCGCAGCACCGTGCGGCGGCTG
CTGGACATCCCCTTCCCCGGCGACTCCGTGCTGCGCTCGGTGATCCTCGCCGACGTGCTG
ATGAAGGAGGCGCCCGCCGACCTGCTCACCGTGGACGCCAACGAGCACGGGTTCGCCTTC
GTCGCCCCCTTCGGCGACGGCTACTACCGCGTGCTCGCCTGGAACCGGCACACCGACAGC
TCGGCGGACGAGTCCGTGGACTTCGAGGAGCTGCGCCGGCTGTGCCGGGCCATTCTCGGC
CGGGACTTCGGCATGCACGAGCCGCGCTGGGTCTCGCGCTTCCACAGCGACGAGCGCCAG
GCCACCCGGTACCGCGAGGGCCGCTTCTTCCTGGCCGGCGACGCCGCGCACGTCCACTCG
CCGGCCGGCGGCATGGGCATGAACACCGGCCTGCAGGACGCGATGAACCTCGCCTGGAAG
CTGGCGGCGGCGGTGCGCGGCCACGGCGACGACGCCCTGCTCGACACCTACGAGGCGGAG
ACCCATCCGGTCGGCGAGGAGGTGCTGCGCACCTCGGGCTCCCTGGTCCGCGCGGCCATC
CGGCAGAGCGGACGCACCCGGCTCGCCCAGCAGGCGATGGTCTGGTTCGCCGGACACCTC
GGCCCGCTCGGCGAGGCACTGGGCCGCCGGGAGGGCCTGGCCGTCTCCGGCACCGGTATC
ACCTACGGCCGGCAGCGCGGCGACCACCGGCTCGTCGGCCGGTTCTTCCACGGGCGGCTG
ACCGGCGGACGGCTCGACGGCGCCCTGGGCACCGGCCGGTTCGTGCTGCTCAGCCCCGAC
GAGGCCGTGCGCACGGCGGTGGCGGACTGGTCCGACCGCGTCGTGACCGCCCCCGCGGAC
GTCCGCACCACGCTGCTCATCCGGCCGGACGGGTACGTCGCCTGGGCGGCCGACGGCCCC
GGCCCGGCGGCGGTCCGCGACGCCCTGAACCGCTGGGCGGGCCCGGCGGAACCCCGGATC
CGGCTTGCGTACGCGGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002938 Monooxygenase, FAD-binding (Domain) PF01494 FAD_binding_3 IPR003042 Aromatic-ring hydroxylase-like (Domain) PR00420 RNGMNOXGNASE
SignalP	Eukaryota Bacteria, Gram-positive
TMHMM	No significant hit

Ovied_00160 Ovied_00180