Ovied_00170 Ovied_00170   Ovied_00190 Ovied_00190

Ovied_00180 : CDS information

close this sectionLocation

Organism
StrainATCC 11891
Entry nameOviedomycin
Contig
Start / Stop / Direction17,521 / 18,402 / + [in whole cluster]
17,521 / 18,402 / + [in contig]
Location17521..18402 [in whole cluster]
17521..18402 [in contig]
TypeCDS
Length882 bp (293 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative phosphopantetheinyl transferase
Product (GenBank)4'-phosphopantetheinyl transferase
Gene
Gene (GenBank)ovmF
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15368568] Genetic organization of the biosynthetic gene cluster for the antitumor angucycline oviedomycin in Streptomyces antibioticus ATCC 11891. (Chembiochem. , 2004)
comment
OvmF: phosphopantetheinyl transferase, lipid-polyketide-metabolism

oviedomycin生合成gene clusterの報告。
OvmFについては配列解析のみ。
Related Reference
ACC
Q9L7M4
NITE
Jado_00180
PmId
[11390684] Cloning and functional analysis of a phosphopantetheinyl transferase superfamily gene associated with jadomycin biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 2001)
comment
Blast 8th, id46%, 4e-41, gapあり
Streptomyces venezuelae_jadM
Phosphopantetheinyl transferase

jadomycin B 生産菌Streptomyces venezuelae ISP5230にあるjadMをE.coliにて発現、クローニング。

jadM欠損株は、jadomycin生産がwild-typeの2-5%程度に落ちた。
また、この欠損株の生育は良く、chloramphenicolの生産には変化がなかったことから、jadMがコードしているholo-ACP synthaseはjadomycin B合成の初期段階に関与しているのではないかと言っている。

holo-ACP synthaseであるというのは配列解析(その他PPTaseとのalignment、系統樹）から。
ACC
Q9RFL1
NITE
Myxo_00010
PmId
[10601310] New lessons for combinatorial biosynthesis from myxobacteria. The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1. (J Biol Chem. , 1999)
[11134965] The mtaA gene of the myxothiazol biosynthetic gene cluster from Stigmatella aurantiaca DW4/3-1 encodes a phosphopantetheinyl transferase that activates polyketide synthases and polypeptide synthetases. (J Biochem. , 2001)
comment
Blast 68th, id32%, 2e-13, gapあり
Stigmatella aurantiaca_mtaA
MtaA
[Myxo_00010]phosphopantetheinyl transferase

--
[PMID: 10601310](1999)
Myxothiazol生合成gene clusterの同定論文。
MtaA: Ppant transferase
Ppant=4'-phosphopantetheinyl

4'-phosphopantetheinyl transferase MtaAによってmyxothiazol合成は活性化される。
mtaAへのkanamycin resistance geneの挿入はmyxothiazol合成を阻害する。
MtaB-MtaGのACP, PCP domainは4'-phosphopantetheine cofactorのためのputative binding siteのProsite consensus signatureを含んでいる。

--
[PMID: 11134965](2001)
MtaAをACP, PCPと異種性発現して、4'-phosphopantetheinyl transferases機能があることを確認。
MtaAは他種のACP, PCPも同様に加工でき、基質特異性がゆるい。
Family/Domain
FD
IPR008278
comment
[IPR008278] 4'-phosphopantetheinyl transferase (Domain)
function; magnesium ion binding, holo-[acyl-carrier-protein] synthase activity

close this sectionSequence

selected fasta
>putative phosphopantetheinyl transferase [4'-phosphopantetheinyl transferase]
MPDGRRVDLWLLRCPGPGTPPGPHVRAGPHVRADTRVRPDPDVRPAPDPLADPGVLDVLD
AAERKRAASLRRPADRALYVASHLALRTLLGAYLRVPAREVVLVREPCPGCGGPHGRPAV
PGTPPPLHFSLSHSHGLALIGVAAVPVGVDVQRVPGPGTVDACAPSLHPAERAELERLPP
GARPGAFAQLWARKEAYLKGIGTGLSRPASADYLGADTRSRPHGWTVTDLPRADGHTAAV
AVLGTAPPAPAVRRLPVRLTSPHRLTTMHQAANDHEPTDQLARQPEGTHEETA
selected fasta
>putative phosphopantetheinyl transferase [4'-phosphopantetheinyl transferase]
GTGCCGGACGGCCGGCGCGTGGACCTGTGGCTGCTGCGCTGCCCCGGACCGGGCACCCCG
CCCGGCCCTCACGTACGGGCCGGTCCTCACGTACGGGCCGACACCCGCGTACGCCCCGAC
CCGGACGTACGCCCCGCCCCGGACCCTCTCGCCGACCCGGGCGTCCTCGACGTACTCGAC
GCGGCGGAGCGGAAGCGGGCGGCCTCCCTGCGCCGGCCCGCCGACCGGGCCCTCTACGTC
GCCTCGCACCTCGCCCTGCGCACCCTGCTCGGCGCCTACCTGCGCGTCCCCGCGCGGGAG
GTGGTCCTCGTCCGCGAGCCGTGCCCGGGGTGCGGCGGCCCGCACGGCCGCCCGGCGGTC
CCGGGCACCCCGCCCCCGCTGCACTTCTCCCTCTCGCACAGCCACGGCCTGGCCCTGATC
GGGGTGGCGGCGGTCCCCGTCGGCGTCGATGTGCAGCGGGTGCCGGGTCCCGGCACCGTC
GACGCCTGCGCCCCTTCGCTGCACCCGGCCGAACGCGCCGAACTGGAGCGGCTGCCGCCC
GGCGCACGCCCCGGCGCGTTCGCCCAGCTGTGGGCGCGCAAGGAGGCGTACCTCAAGGGC
ATCGGCACCGGCCTGAGCCGCCCCGCGTCCGCCGACTACCTCGGCGCCGACACCCGGTCC
CGGCCGCACGGCTGGACGGTCACCGACCTGCCCCGCGCCGACGGCCACACCGCGGCGGTC
GCCGTCCTCGGCACGGCGCCCCCGGCCCCGGCCGTGCGCCGGCTGCCCGTGCGCCTCACC
TCACCTCACCGACTGACGACCATGCACCAGGCCGCCAACGACCACGAGCCCACCGACCAG
TTGGCAAGGCAACCGGAGGGTACCCATGAGGAAACAGCTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR008278 4'-phosphopantetheinyl transferase (Domain)
[146-211] 1.3e-08 G3DSA:3.90.470.20
G3DSA:3.90.470.20   G3DSA:3.90.470.20
[146-206] 9.10000000000001e-09 PF01648
PF01648   ACPS
[55-151] 3.00000067992871e-21 SSF56214 [145-264] 1.89999859865865e-23 SSF56214
SSF56214   4-PPT_transf
SignalP No significant hit
TMHMM No significant hit
Ovied_00170 Ovied_00170   Ovied_00190 Ovied_00190
Page top