Oxzol_00130 : CDS information

Location

Organism	Streptomyces albus
Strain	JA3453
Entry name	Oxazolomycin
Contig	EF552687
Start / Stop / Direction	19,437 / 18,574 / - [in whole cluster] 19,437 / 18,574 / - [in contig]
Location	complement(18574..19437) [in whole cluster] complement(18574..19437) [in contig]
Type	CDS
Length	864 bp (287 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative oxidoreductase
Product (GenBank)	glyceroyl ACP dehydrogenase
Gene
Gene (GenBank)	ozmG
EC number
Keyword	methoxymalonyl-ACP
Note
Note (GenBank)
Reference	ACC B2WW41 PmId [16707707] Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning the oxazolomycin biosynthetic gene cluster from Streptomyces albus JA3453. (J Bacteriol. , 2006) [20406823] Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units. (J Biol Chem. , 2010) comment [PMID:16707707] methoxymalonyl-ACP生合成遺伝子の同定とクローニング OzmB, OzmC, OzmD, OzmE, OzmF, OzmGが、methoxymalonyl-ACP生合成遺伝子であることを同定し、これらがoxazolomycin productionに必須であることを示した。 OzmGはHADH family enzymeに相同性を示すが、機能解析はされていない。 [PMID:20406823] oxazolomycin生合成遺伝子クラスターの報告。 ozmG: 3-Hydroxyacyl-CoA-dehydrogenase methoxymalonyl-ACP生合成経路の三番目を担い、glyceryl-ACPをhydroxymalonyl-ACPへ変換させるための酸化に関与すると推測している(FIGURE 3.)。機能解析はされていない。
Related Reference	ACC Q8KUG1 NITE Ansam_00150 PmId [11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002) comment Actinosynnema pretiosum subsp. auranticum_asm13 3-hydroxyacyl-CoA dehydrogenase S. lividansで、AT domainsのひとつがmethoxymalonateに特異的なATで置換されているerythromycin PKSのカセットとasm13-17を同時発現すると、予測された位置にmethoxymalonateが取り込まれた6- deoxyerythronolide Bの派生体が産生された。よって、asm13-17はmethoxymalonyl-ACP生合成に十分である。
	ACC Q9ADL9 NITE Soraph_00040 PmId [12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002) [15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004) comment Sorangium cellulosum_sorD Beta-hydroxybutyryl-CoA dehydrogenase [PMID: 12039053](2002) Soraphen A biosynthetic gene clusterの報告。 SorD(295aa): beta-Hydroxybutyryl-CoA dehydrogenase Module 3,7で取り込まれるMethoxymalonyl-CoAを合成する。本ORFはCH2OHをCHOにする --- [PMID: 15289572](2004) sorC破壊株はsoraphen A非産生。 S. lividansでのsor genes異種性発現。 SorABRCDFE株 + cinnamate SorABRCDFE株 + badA(benzoate-CoA ligase) + benzoate or cinnamate の組み合わせでSoraphen A産生。 SorABRCDFE株で発現されているのは、the sorA, and the sorB4M, sorRCDFE operons.
	ACC Q7BQ74 PmId [16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006) comment Bacillus cereus ZmaG(zmaG) ZmaGは、珍しいPKS伸長units, hydroxymalonyl-ACP and aminomalonyl-ACPの生合成に共通して関連する。 ZmaE or ZmaIと共に働く。 ZmaI: eryl-ZmaH(ACP)→aminomalonyl-ZmaH(ACP) ZmaE: glyceryl-ZmaD(ACP)→hydroxymalonyl-ZmaD(ACP)

Sequence

>putative oxidoreductase [glyceroyl ACP dehydrogenase]
MTEEHPTPRLAVLGAGVMGVGITTLALGQGVPVVLVETDPAKRADAPARVERELRTAQLM
GVRSGAPRAELVTAARPADCAGADVVIEAITEDAGLKAAALTEVSEAVGEDTALVTNTSS
IPVDELAAKLPRPEDLLGVHFMNPSYLIRTVEVIRGSRTSARAVDSVTRLLTALGRRGVL
VGDGPGFVTSRVLHRMINDAARIVQEGRASAEDVDTLMQDCLGHRTGPLRTADLIGIDNL
VDSLRVLHERTGDEGCRPCELLLQKVRDGEHGRKSGQGFYTYTGVVS

>putative oxidoreductase [glyceroyl ACP dehydrogenase]
GTGACAGAGGAACATCCCACCCCACGGCTGGCCGTTCTGGGCGCCGGTGTCATGGGGGTC
GGCATCACCACACTCGCTCTCGGTCAGGGCGTCCCGGTGGTCCTGGTCGAAACCGATCCG
GCGAAGCGCGCGGACGCCCCGGCCAGGGTCGAGCGCGAACTGCGCACGGCCCAGCTCATG
GGCGTCCGCTCCGGCGCCCCCCGCGCCGAGCTGGTCACCGCCGCCCGGCCGGCGGACTGC
GCGGGCGCGGACGTGGTGATCGAGGCGATCACCGAGGACGCCGGCCTGAAGGCCGCGGCG
CTGACCGAGGTGAGCGAAGCCGTCGGTGAGGACACCGCCCTGGTCACCAACACCTCCTCC
ATCCCGGTGGACGAGCTCGCGGCGAAGCTCCCGCGCCCCGAGGACCTGCTCGGCGTCCAC
TTCATGAACCCCTCGTACCTGATCCGCACGGTCGAGGTGATCCGCGGCTCGCGGACCTCG
GCACGGGCCGTGGACTCCGTCACCAGGCTCCTCACCGCACTCGGCCGCCGCGGCGTCCTC
GTCGGCGACGGCCCCGGGTTCGTGACCAGCCGCGTACTGCACCGCATGATCAACGACGCG
GCCCGCATCGTGCAGGAGGGCCGCGCGAGCGCCGAGGACGTCGACACCCTGATGCAGGAC
TGCCTCGGGCACCGCACCGGGCCGCTGCGCACCGCCGACCTGATCGGCATCGACAACCTC
GTCGACTCCCTGCGGGTGCTGCACGAGCGCACCGGCGACGAAGGCTGCCGCCCGTGCGAG
CTGCTGCTCCAGAAGGTGCGCGACGGCGAGCACGGCCGCAAGAGCGGCCAGGGCTTCTAC
ACGTACACGGGAGTGGTCTCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain) PF00725 3HCDH IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain) PF02737 3HCDH_N IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR013328 Dehydrogenase, multihelical (Domain) G3DSA:1.10.1040.10 Opine_DH IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family) PIRSF000105 HCDH
SignalP	Bacteria, Gram-negative Eukaryota Bacteria, Gram-positive
TMHMM	Transmembrane 1

Oxzol_00120 Oxzol_00140