Oxzol_00110 Oxzol_00110   Oxzol_00130 Oxzol_00130

Oxzol_00120 : CDS information

close this sectionLocation

Organism
StrainJA3453
Entry nameOxazolomycin
Contig
Start / Stop / Direction18,574 / 17,909 / - [in whole cluster]
18,574 / 17,909 / - [in contig]
Locationcomplement(17909..18574) [in whole cluster]
complement(17909..18574) [in contig]
TypeCDS
Length666 bp (221 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative O-methyltransferase
Product (GenBank)o-methyltransferase
Gene
Gene (GenBank)ozmF
EC number2.1.1.-
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
Reference
ACC
PmId
[16707707] Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning the oxazolomycin biosynthetic gene cluster from Streptomyces albus JA3453. (J Bacteriol. , 2006)
[20406823] Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units. (J Biol Chem. , 2010)
comment
[PMID:16707707]
methoxymalonyl-ACP生合成遺伝子の同定とクローニング

OzmB, OzmC, OzmD, OzmE, OzmF, OzmGが、methoxymalonyl-ACP生合成遺伝子であることを同定し、これらがoxazolomycin productionに必須であることを示した。
OzmFに関して機能解析はされていない。


[PMID:20406823]
oxazolomycin生合成遺伝子クラスターの報告。

ozmF: O-methyltransferase

methoxymalonyl-ACP生合成経路の最後を担うと推測している(FIGURE 3.)。機能解析はされていない。
Related Reference
ACC
Q8KUG5
NITE
Ansam_00190
PmId
[11996558] Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. (J Am Chem Soc. , 2002)
comment
Actinosynnema pretiosum subsp. auranticum_asm17
O-methyltransferase

S. lividansで、AT domainsのひとつがmethoxymalonateに特異的なATで置換されているerythromycin PKSのカセットとasm13-17を同時発現すると、予測された位置にmethoxymalonateが取り込まれた6- deoxyerythronolide Bの派生体が産生された。よって、asm13-17はmethoxymalonyl-ACP生合成に十分である。
また、methyltransferaseをコードするasm17を削除すると、そのhydroxy類似体ではなく6-deoxyerythronolide Bが得られたので、伸長unitのmethylationはその取り込みのために必要である。
ACC
P28034
PmId
[1894629] Molecular cloning, induction and taxonomic distribution of caffeoyl-CoA 3-O-methyltransferase, an enzyme involved in disease resistance. (J Biol Chem. , 1991)
[9037150] Structure of the parsley caffeoyl-CoA O-methyltransferase gene, harbouring a novel elicitor responsive cis-acting element. (Plant Mol Biol. , 1997)
comment
Petroselinum crispum (Parsley)(パセリ)
Caffeoyl-CoA O-methyltransferase(EC 2.1.1.104)

caffeoyl-CoA-specific 3-O-methyltransferaseの活性測定あり。
Family/Domain
FD
IPR002935
comment
[IPR002935] O-methyltransferase, family 3 (Family)

close this sectionSequence

selected fasta
>putative O-methyltransferase [o-methyltransferase]
MAGQLSLDEPLLDYVRQMSLRDDDVLRDLRAETAGMPMLRAMLVLPEEAQLLALLVRVTG
ARNVLEVGTFTGYSSLCMARALPPGGRVVTCDISERWTAVARRYWERAGVDGLIDLRLGD
AAETLDTLCAQDGPGGFDLAFVDADKANYLRYYEQSLALVRPGGLLVLDNTLFFGKVVDP
AAQDPDTLAIRELNTLLRDDERVDLSMLAVADGLTLVHKKP
selected fasta
>putative O-methyltransferase [o-methyltransferase]
ATGGCGGGACAGCTCTCACTCGACGAGCCGCTCCTCGACTACGTACGGCAGATGTCGCTG
CGCGACGACGACGTGCTGCGCGACCTGCGCGCCGAGACCGCGGGCATGCCCATGCTCCGG
GCGATGCTGGTCCTGCCCGAAGAGGCACAGCTCCTCGCGCTCCTCGTACGGGTCACCGGG
GCGCGGAACGTCCTGGAGGTCGGCACCTTCACCGGCTACAGCAGCCTGTGCATGGCACGC
GCCCTGCCGCCCGGCGGCCGGGTCGTCACCTGCGACATCAGCGAGCGCTGGACGGCCGTC
GCCCGGCGCTACTGGGAGCGGGCCGGGGTCGACGGGCTGATCGACCTGCGGCTCGGGGAC
GCCGCGGAGACGCTCGACACCCTGTGCGCGCAGGACGGCCCCGGCGGCTTCGACCTGGCC
TTCGTCGACGCGGACAAGGCCAACTACCTCCGTTACTACGAGCAGTCGCTCGCCCTGGTC
CGCCCCGGCGGACTGCTCGTCCTCGACAACACCCTCTTCTTCGGCAAGGTCGTCGACCCC
GCCGCGCAGGACCCGGACACCCTGGCCATCCGTGAACTCAACACCCTGCTGCGGGATGAC
GAGCGCGTGGACCTCTCCATGCTCGCCGTCGCCGACGGCCTCACCCTCGTCCACAAGAAG
CCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002935 O-methyltransferase, family 3 (Family)
[17-219] 9.60000000000004e-83 PF01596
PF01596   Methyltransf_3
SignalP No significant hit
TMHMM No significant hit
Oxzol_00110 Oxzol_00110   Oxzol_00130 Oxzol_00130
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