Oxzol_00270 : CDS information

Location

Organism	Streptomyces albus
Strain	JA3453
Entry name	Oxazolomycin
Contig	EF552687
Start / Stop / Direction	95,512 / 96,849 / + [in whole cluster] 95,512 / 96,849 / + [in contig]
Location	95512..96849 [in whole cluster] 95512..96849 [in contig]
Type	CDS
Length	1,338 bp (445 aa)

Annotation

Category	5.1 general function
Product	putative cytochrome P450
Product (GenBank)	cytochrome P450
Gene
Gene (GenBank)	ozmV
EC number
Keyword
Note	This ORF is not involved in oxazolomycin biosynthesis.
Note (GenBank)
Reference	ACC B2WW55 PmId [20406823] Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units. (J Biol Chem. , 2010) comment oxazolomycin生合成遺伝子クラスターの報告。 orf(+1): Cytochrome P450 NCBI contig(EF552687)では、ozmVと表記されている。生合成遺伝子の境界を決定するため、orf(-1), orf(-2), orf(-3)-orf(-5),orf(+1), orf(+2), orf(+3)-orf(+5)欠損株作製。いずれもoxazolomycin 産生にいかなる影響も与えなかったことから、oxazolomycin生合成には関与しないと結論づけている。
Related Reference	ACC O31440 PmId [10529095] ( , ) [12519760] ( , ) comment Bacillus subtilis Cytochrome P450 152A1(cypC) [PMID:10529095] Sphingomonas paucimobilis由来Fatty acid alpha-hydroxylase(O24782)の配列を元に相同性検索。クローニング、酵素活性測定。 Bacillus subtilis (P450(BSbeta))はoxidantとしてhydrogen peroxideを利用しalpha- and beta-positionsでlong-chain fatty acidをヒドロキシル化することを示した。 [PMID:12519760] 結晶構造解析にてmetal ion-binding site決定。
Related Reference	ACC O24782 PmId [9295298] ( , ) comment Pseudomonas paucimobilis Fatty acid alpha-hydroxylase Fatty acid alpha-hydroxylaseの解析。相同性からP450の一種だが、今までにみつかっていないfamilyであることを証明。

Sequence

>putative cytochrome P450 [cytochrome P450]
MTCTGAPVPAGFWETPPPVHVTARVCVTQEDLMLDNTLPLLTRGYAWLPDLSRREGPGAV
RTRLMGKPAIALRGPAAVGFFYDEDHVRRRTALPEPVLATLFGKTAVHTLDGAEHRKRKE
LFIKVLKDTSGVTALAERVADEWERSCKEWSDRARVTLFDEVSVLITRAVCAWAGVPLTE
GPEGDARRTAQDLVLMVDGFATAGPRHWRARRARQRQEERLARLVEDIRSAGGKTTGEGG
PPTTAVEAVAVHRDADGELLEPRTAAVEILNIIRPTVAVTWYTVFGAHALHRNPSLRQRL
ATGGEGYARAFAHEVRRFYPFAPFVAGLAPRDVEWHGEHIAEGSLVLLDLYGQNHDPELW
EAPYTFDPERFVGREPGRDELVPQGGGEASQGHRCPGEDVTLAVLSTLLPRLARLDYRVP
DQDLRIPLNRMPTRPRSGFVIAETH

>putative cytochrome P450 [cytochrome P450]
GTGACATGCACGGGTGCCCCGGTTCCGGCAGGCTTCTGGGAGACGCCACCACCCGTCCAC
GTCACGGCCCGCGTATGTGTCACCCAGGAGGACCTCATGCTGGACAACACGCTGCCGCTC
CTGACGCGGGGCTACGCGTGGCTTCCCGATCTGAGCCGCCGCGAGGGTCCGGGCGCCGTC
CGCACCCGTCTGATGGGCAAGCCCGCCATCGCGCTGCGCGGCCCCGCCGCCGTCGGGTTC
TTCTACGACGAGGACCATGTACGCCGTCGCACGGCCCTGCCCGAACCCGTTCTCGCCACC
CTCTTCGGCAAGACCGCGGTGCACACCCTGGACGGGGCGGAGCACCGTAAACGCAAGGAA
CTGTTCATCAAGGTGCTCAAGGACACCTCCGGCGTCACCGCATTGGCGGAGCGGGTCGCC
GACGAGTGGGAGCGGTCCTGCAAGGAGTGGTCCGACCGGGCCAGGGTGACGCTCTTCGAC
GAGGTGAGTGTCCTGATCACCCGGGCCGTGTGCGCCTGGGCGGGGGTCCCGCTCACCGAA
GGACCCGAGGGCGACGCGCGGCGGACCGCCCAGGACCTGGTCCTCATGGTCGACGGTTTC
GCCACGGCGGGCCCGCGGCACTGGCGGGCCCGGCGCGCCAGGCAGCGCCAGGAGGAGCGG
CTGGCCCGGCTGGTCGAGGACATCAGGTCGGCCGGCGGCAAGACGACCGGCGAGGGCGGC
CCGCCGACCACCGCCGTGGAGGCCGTGGCCGTCCACCGGGACGCCGACGGCGAGCTCCTC
GAACCGCGTACGGCGGCGGTGGAGATCCTCAACATCATCCGGCCGACGGTCGCCGTCACC
TGGTACACGGTGTTCGGCGCCCACGCCCTGCACCGCAACCCGTCGCTGCGGCAGCGCCTG
GCCACGGGCGGGGAGGGCTACGCGCGAGCGTTCGCCCACGAGGTGCGGCGCTTCTACCCG
TTCGCACCGTTCGTCGCGGGCCTCGCCCCGCGCGACGTCGAATGGCACGGCGAGCACATC
GCCGAGGGCTCCCTGGTCCTCCTCGACCTGTACGGGCAGAACCACGACCCCGAGCTGTGG
GAGGCCCCGTACACCTTCGACCCCGAGCGGTTCGTGGGCCGCGAGCCGGGGCGCGACGAG
CTCGTGCCGCAGGGCGGCGGCGAGGCCTCCCAGGGGCATCGCTGCCCGGGCGAGGACGTC
ACGCTGGCGGTCCTGAGCACCCTGCTGCCGCGCCTGGCGCGGCTCGACTACCGGGTGCCC
GACCAGGATTTGCGCATCCCGTTGAACCGGATGCCCACCAGGCCGCGCAGCGGTTTCGTC
ATCGCGGAGACGCACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) SSF48264 Cytochrome_P450 PF00067 p450 G3DSA:1.10.630.10 Cyt_P450 IPR002401 Cytochrome P450, E-class, group I (Family) PR00463 EP450I
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Oxzol_00260 Oxzol_00280