Oxzol_00310 : CDS information

Location

Organism	Streptomyces albus
Strain	JA3453
Entry name	Oxazolomycin
Contig	EF552687
Start / Stop / Direction	102,616 / 100,514 / - [in whole cluster] 102,616 / 100,514 / - [in contig]
Location	complement(100514..102616) [in whole cluster] complement(100514..102616) [in contig]
Type	CDS
Length	2,103 bp (700 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	putative oxidoreductase
Gene
Gene (GenBank)
EC number
Keyword
Note	This ORF is not involved in oxazolomycin biosynthesis.
Note (GenBank)	ozm orf +3
Reference	ACC B2WW59 PmId [20406823] Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units. (J Biol Chem. , 2010) comment oxazolomycin生合成遺伝子クラスターの報告。 orf(+5): hypothetical protein NCBI contig(EF552687)では、orf(+3)と表記されている。生合成遺伝子の境界を決定するため、orf(-1), orf(-2), orf(-3)-orf(-5),orf(+1), orf(+2), orf(+3)-orf(+5)欠損株作製。いずれもoxazolomycin 産生にいかなる影響も与えなかったことから、oxazolomycin生合成には関与しないと結論づけている。
Related Reference	ACC Q46799 PmId [10986234] ( , ) comment Escherichia coli Xanthine dehydrogenase molybdenum-binding subunit(xdhA) xdhA, b2881(xdhD)それぞれの遺伝子のdeletion mutantを作成し、adenineからのCO2生成の変化を調べている。xdhA mutantではCO2生成の減少が見られ、XdhAはCO2の生成つまりXDH活性に関与しているとしている。 xdhABCと並んでおり、これらはおそらくheterotrimerとなって機能すると思われる。

Sequence

>putative oxidoreductase [putative oxidoreductase]
MPDTTSALGAPTERREGPAKVTGAAPYAAEHTPEGCAYAWPVPATVARGEITSVDDTAAL
AVPGVVGVLTHLNAPRLAEADDAILAVLQDARVPHRGWYVALVVADTLEAAREGAAAVTV
TYSEEPHDVTLDAGSPAAYVPEDPDGNPDEHTRGDPDAALAAAPTQVDVTYEVPPLHNHP
MEPHAATAHWQDGRLTAYDSSQGATAVRQTLASLFKLPEEQVTVVSEHVGGGFGAKGTPR
PHVVLAAMAARETGRPVKLALPRRHLATVVGHRSPTVQRLRLGAEADGTLTSVIHEVTSY
TSRIKEFVETGAAVSRVMYPSPHARTGHRVLPLDVPSPSWMRAPGEAPGMYALESAMDEL
AVALGMDPVELRLRNDTDKEPDSGKPFSSRHLAECLREGARRFGWAERDPRPRSRTEGPL
LIGTGVAAATYPVLVSPSRASARAYPDGGYVVRVNATDIGTGARTVLAQVAADALGVPVD
RVRTEVGSTDLPAAPLAGGSSGTASWGWAVHEACTRLAERLAGQREQRLPPEGVGASADT
EGTSDTNYAESDYARHAFGAHFAEVGVDSVSGEVRVRRLLGVYAAGHILNARTARSQFIG
GMTMGLGMALTEHSTMDEPFGDFAESDLASYHVPVHADVPAIEAHWIDEDDPHLNPMGSK
GIGEIGIVGTAAAIGNAVHHATGVRVRELPVTPDKILTGS

>putative oxidoreductase [putative oxidoreductase]
ATGCCGGACACCACCAGCGCTCTCGGGGCGCCCACCGAGCGCAGGGAGGGCCCCGCCAAG
GTGACAGGGGCCGCCCCCTACGCCGCCGAGCACACGCCCGAGGGCTGCGCCTACGCCTGG
CCCGTGCCCGCCACCGTCGCGCGCGGCGAGATCACCTCCGTCGACGACACGGCGGCCCTC
GCGGTGCCCGGTGTCGTCGGCGTCCTGACGCACCTCAACGCGCCGCGCCTCGCCGAGGCC
GACGACGCCATCCTCGCCGTGCTCCAGGACGCCCGCGTCCCGCACCGGGGCTGGTACGTC
GCCCTCGTCGTCGCCGACACCCTGGAGGCGGCCCGCGAAGGCGCCGCCGCCGTCACGGTC
ACGTACTCCGAGGAACCGCACGACGTCACCCTGGACGCCGGGAGCCCCGCCGCCTACGTG
CCCGAGGATCCCGACGGCAACCCGGACGAGCACACGCGCGGGGACCCCGACGCGGCCCTC
GCCGCCGCGCCCACCCAGGTGGACGTGACATACGAGGTGCCGCCGCTGCACAACCACCCC
ATGGAGCCGCACGCGGCCACCGCCCACTGGCAGGACGGGCGGCTGACCGCGTACGACTCC
AGCCAGGGCGCCACCGCCGTCCGCCAGACCCTGGCGAGCCTCTTCAAGCTGCCCGAGGAG
CAGGTCACCGTCGTCTCCGAACACGTCGGCGGCGGCTTCGGCGCCAAGGGCACGCCACGG
CCGCACGTCGTGCTCGCCGCCATGGCCGCGCGCGAGACGGGGCGGCCGGTCAAACTCGCC
CTGCCCCGGCGCCACCTGGCGACCGTGGTGGGTCACCGTTCGCCGACCGTGCAGCGGCTG
CGGCTCGGGGCGGAGGCGGACGGCACGCTGACGTCCGTGATCCACGAGGTGACCTCGTAC
ACCTCGCGCATCAAGGAGTTCGTGGAGACGGGCGCCGCCGTCTCCCGCGTCATGTATCCC
TCGCCACACGCCCGCACCGGCCACCGGGTGCTGCCGCTCGACGTGCCGAGCCCCTCATGG
ATGCGTGCCCCCGGCGAAGCGCCGGGCATGTACGCCCTGGAGTCCGCCATGGACGAGCTC
GCCGTGGCGCTCGGCATGGACCCCGTCGAGCTGCGGCTGCGCAACGACACCGACAAAGAG
CCCGACAGCGGCAAGCCGTTCAGCAGCCGCCACCTCGCCGAGTGCCTGCGCGAGGGCGCG
CGCCGCTTCGGCTGGGCCGAGCGCGACCCGCGGCCCCGCTCACGGACCGAGGGCCCCCTC
CTGATCGGCACCGGGGTCGCCGCGGCGACCTACCCGGTGCTCGTCTCCCCCTCCCGGGCG
AGCGCCCGCGCCTACCCGGACGGCGGCTACGTGGTCCGGGTCAACGCCACCGACATCGGT
ACGGGCGCCCGTACCGTGCTCGCCCAGGTCGCCGCCGACGCGCTCGGCGTTCCCGTGGAC
CGCGTGCGCACCGAGGTCGGCAGCACCGACCTGCCCGCGGCGCCGCTCGCGGGCGGCTCC
TCCGGCACCGCCTCCTGGGGCTGGGCCGTCCACGAGGCCTGTACGCGGCTCGCCGAGCGC
CTGGCCGGACAGCGGGAGCAGCGACTGCCGCCGGAGGGCGTCGGCGCGTCGGCCGACACG
GAGGGCACGTCCGACACCAACTACGCGGAGAGCGACTACGCGCGGCACGCCTTCGGCGCG
CACTTCGCGGAGGTCGGCGTCGACTCCGTATCGGGCGAGGTCCGTGTGCGCAGGCTGCTC
GGCGTCTACGCGGCGGGGCACATCCTCAACGCCCGTACCGCGCGCTCGCAGTTCATCGGC
GGGATGACCATGGGGCTGGGCATGGCCCTGACCGAGCACAGCACCATGGACGAGCCTTTC
GGTGACTTCGCGGAGAGCGACCTCGCCTCCTACCACGTACCGGTGCACGCCGACGTGCCC
GCGATCGAGGCGCACTGGATCGACGAGGACGACCCGCACCTGAACCCCATGGGCAGCAAG
GGCATCGGTGAGATCGGCATCGTCGGCACGGCCGCGGCGATCGGCAACGCCGTGCACCAT
GCCACCGGGGTGCGGGTGCGGGAGCTGCCGGTCACACCGGACAAGATCCTGACCGGTTCC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000674 Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead (Domain) G3DSA:3.90.1170.50 Aldxan_DH_hamm SSF54665 Aldxan_dh_hamm SM01008 Ald_Xan_dh_C PF01315 Ald_Xan_dh_C IPR008274 Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding (Domain) G3DSA:3.30.365.10 Ald_xan_DH_mo_bd SSF56003 Ald_xan_DH_mo_bd PF02738 Ald_Xan_dh_C2
SignalP	No significant hit
TMHMM	No significant hit

Oxzol_00300 Oxzol_00320