Pact_00010 : CDS information

Location

Organism	Streptomyces pactum
Strain	ATCC 27456 (=NBRC 13433)
Entry name	Pactamycin
Contig	AB303063
Start / Stop / Direction	2 / 1,882 / + [in whole cluster] 2 / 1,882 / + [in contig]
Location	2..1882 [in whole cluster] 2..1882 [in contig]
Type	CDS
Length	1,881 bp (626 aa)

Annotation

Category	5.1 general function
Product	putative helicase(fragment)
Product (GenBank)	ATP-dependent RNA helicase
Gene	orf1
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in pactamycin biosynthesis.
Note (GenBank)	orf1
Reference	ACC A8R0I3 PmId [17827660] Cloning of the pactamycin biosynthetic gene cluster and characterization of a crucial glycosyltransferase prior to a unique cyclopentane ring formation. (J Antibiot (Tokyo). , 2007) comment Pactamycin生合成遺伝子クラスターの報告。 Orf1: ATP-dependent RNA helicase orf1とpctAの間に存在する1926bpのnon-coding regionがpct clusterの境界になるかもしれないと述べている。遺伝子欠損株によるPactamycinの発現確認はしていない。
Related Reference	ACC B7TWK5 PmId [19670201] Deciphering pactamycin biosynthesis and engineered production of new pactamycin analogues. (Chembiochem. , 2009) comment S.pactum ATCC27456のPactamycin生合成遺伝子クラスターの報告。 Orf16: ATP-dependent RNA helicase このORF(Pact_00010)よりもN末配列が長く登録されており、Orf16としている。Orf16の機能解析はされていない。この株ではPtmA-Zがpactamaycin生合成遺伝子だとしており、このOrf16は生合成遺伝子だとしていない。
Family/Domain	FD IPR011545, IPR001650 comment [IPR011545] DNA/RNA helicase, DEAD/DEAH box type, N-terminal (Domain) [IPR001650] DNA/RNA helicase, C-terminal (Domain)

Sequence

>putative helicase(fragment) [ATP-dependent RNA helicase]
MLTPTRELAMQVSDALQPYGDVLGLKLKVVCGGTSMGNQIYALERGVDILVATPGRLRDI
IDRGAASLDRVQVAVLDEADQMADMGFLPEVTEILDLVPQGGQRLLFSATLENEIDTLVK
RYLVDPVTHEVDPSAGAVSTMTHHVLVVKPKDKAPVTAAIAARKGRTIIFVRTQLGADRV
AEQLRDSGVRADALHGGMTQGARTRTLADFKDGYVNVLVATDVAARGIHVDGIDLVLNVD
PAGDHKDYLHRSGRTARAGQSGTVVSLALPHQRRQIFRLMEDAGVDASRHIVGGAGAFDE
DVARITGARSLTEVQAESAANSAKQAEREVEQLTRELERVQRRATELREEADRLAARAAR
ERGEDPQAAVAPAEPAADGAEAPAAAPSVPEQTAAPVVENTVADEAPRRTGPERRDERGS
YERRDRGGDDRGGFGRDRDRRDDRPFNRDRRDDRGGFGRERRDGDRDRGFGRDRDRERPS
FRDRRDGDRDRRDGERGGSGGRSYERRDRDDRGFGRDRDDRGGFNRDRDRRDDRPFNRDR
RDDRGGFGRERREDRPGRPFERRDHAPRDHHRGGDRPFNRDRRDDRPFGRDRRDDRPARR
DDHRGGTTGSRSFDRRADKPRWKRNG

>putative helicase(fragment) [ATP-dependent RNA helicase]
ATCCTCACGCCCACCCGTGAGCTGGCCATGCAGGTGAGCGACGCGCTCCAGCCGTACGGC
GACGTCCTGGGCCTGAAGCTCAAGGTCGTCTGCGGCGGCACCTCGATGGGCAACCAGATC
TACGCCCTGGAGCGCGGGGTGGACATCCTCGTCGCGACCCCGGGCCGGCTGCGCGACATC
ATCGACCGCGGCGCCGCGTCGCTGGACCGCGTGCAGGTCGCGGTGCTGGACGAGGCGGAC
CAGATGGCCGACATGGGCTTCCTGCCCGAGGTCACCGAGATCCTCGACCTGGTGCCGCAG
GGCGGCCAGCGGCTGCTGTTCTCCGCGACGCTGGAGAACGAGATCGACACCCTGGTCAAG
CGCTACCTGGTCGACCCGGTGACGCACGAGGTGGACCCGTCCGCCGGCGCGGTCTCCACG
ATGACCCACCACGTGCTGGTGGTGAAGCCGAAGGACAAGGCCCCGGTCACCGCCGCCATC
GCCGCCCGCAAGGGCCGCACCATCATCTTCGTCCGCACCCAGCTGGGCGCGGACCGGGTG
GCCGAGCAGCTGCGGGACTCCGGGGTGCGGGCCGACGCCCTGCACGGCGGCATGACCCAG
GGCGCCCGGACCCGGACCCTCGCCGACTTCAAGGACGGTTACGTCAACGTCCTGGTCGCC
ACCGACGTCGCCGCGCGCGGTATCCACGTGGACGGCATCGACCTGGTGCTCAACGTGGAC
CCGGCCGGCGACCACAAGGACTACCTGCACCGCAGTGGGCGAACCGCCCGCGCCGGGCAG
AGCGGCACCGTGGTCTCGCTCGCCCTGCCGCACCAGCGGCGGCAGATCTTCCGGCTGATG
GAGGACGCGGGCGTGGACGCCTCGCGCCACATCGTCGGCGGGGCCGGCGCGTTCGACGAG
GACGTGGCCCGGATCACCGGCGCCCGGTCGCTCACCGAGGTGCAGGCCGAGTCGGCGGCG
AACTCCGCCAAGCAGGCCGAGCGCGAGGTGGAGCAGCTCACCCGCGAACTGGAGCGGGTG
CAGCGCCGCGCCACCGAGCTGCGCGAGGAGGCCGACCGGCTGGCCGCCCGGGCCGCGCGC
GAGCGTGGCGAGGACCCGCAGGCCGCCGTCGCCCCGGCCGAGCCGGCCGCCGACGGGGCC
GAGGCCCCGGCCGCCGCGCCGTCGGTGCCCGAGCAGACCGCCGCCCCCGTGGTGGAGAAC
ACCGTCGCGGACGAGGCGCCGCGCCGTACGGGCCCGGAGCGCCGGGACGAGCGGGGCTCC
TACGAGCGCCGTGACCGGGGCGGGGACGACCGGGGCGGCTTCGGCCGGGACCGGGACCGC
CGGGACGACCGTCCGTTCAACCGTGACCGCCGGGACGACCGGGGCGGCTTCGGCCGGGAG
CGCAGGGACGGCGACCGGGACCGTGGCTTCGGCCGTGACCGCGACCGCGAGCGTCCGTCG
TTCCGCGACCGCCGGGACGGCGACCGTGACCGCCGGGACGGCGAGCGCGGTGGTTCCGGT
GGCCGTTCGTACGAGCGCCGCGACCGCGACGACCGCGGCTTCGGCCGGGACCGCGACGAC
CGGGGCGGCTTCAACCGCGACCGGGACCGCCGGGACGACCGTCCGTTCAACCGTGACCGC
CGGGACGACCGCGGCGGCTTCGGCCGGGAGCGCCGGGAGGACCGCCCGGGCCGTCCGTTC
GAGCGCCGTGACCACGCCCCCCGGGACCACCACCGGGGCGGGGACCGTCCGTTCAACCGT
GACCGCCGCGACGACCGCCCGTTCGGCCGGGACCGCCGGGACGACCGTCCGGCCCGTCGT
GACGACCACCGGGGCGGCACCACCGGCAGCCGGTCCTTCGACCGCCGCGCCGACAAGCCC
CGCTGGAAGCGCAACGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001650 Helicase, C-terminal (Domain) PS51194 HELICASE_CTER SM00490 helicase superfamily c-terminal domain PF00271 Helicase_C IPR011545 DNA/RNA helicase, DEAD/DEAH box type, N-terminal (Domain) PF00270 DEAD IPR014001 Helicase, superfamily 1/2, ATP-binding domain (Domain) PS51192 HELICASE_ATP_BIND_1 SM00487 DEAD-like helicases superfamily
SignalP	No significant hit
TMHMM	No significant hit

first Pact_00020