Pact_00100 Pact_00100   Pact_00120 Pact_00120

Pact_00110 : CDS information

close this sectionLocation

Organism
StrainATCC 27456 (=NBRC 13433)
Entry namePactamycin
Contig
Start / Stop / Direction14,089 / 16,074 / + [in whole cluster]
14,089 / 16,074 / + [in contig]
Location14089..16074 [in whole cluster]
14089..16074 [in contig]
TypeCDS
Length1,986 bp (661 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.2 modification methylation
Productputative C-methyltransferase
Product (GenBank)methyltransferase
GenepctJ
ptmH
Gene (GenBank)pctJ
EC number2.1.1.-
Keyword
  • hydroxyethyl unit
Note
Note (GenBank)
Reference
ACC
PmId
[17827660] Cloning of the pactamycin biosynthetic gene cluster and characterization of a crucial glycosyltransferase prior to a unique cyclopentane ring formation. (J Antibiot (Tokyo). , 2007)
[19670201] Deciphering pactamycin biosynthesis and engineered production of new pactamycin analogues. (Chembiochem. , 2009)
[21513878] Biosynthetic studies and genetic engineering of pactamycin analogs with improved selectivity toward malarial parasites. (Chem Biol. , 2011)
comment
[PMID:17827660]
Pactamycin生合成遺伝子クラスターの報告。

PctJ: C-methyltransferase

相同性より機能付けされている。
このORFの機能解析はされていない。

シクロペンタン環の修飾過程において、3つのmethyltransferases PctJ(このORF), PctN(Pact_00150), PctO(Pact_00160)によりcyclopentane ringに3つの炭素を付着させる反応を行なうと推測している。


[PMID:19670201]
S.pactum ATCC27456のPactamycin生合成遺伝子クラスターの報告。

PtmH: Radical SAM+B12 binding domain

PtmHの機能解析はされていない。


[PMID:21513878(2011)]
putative tailoring genesの解析報告(S.pactum ATCC27456)。

PtmH: SAM-dependent C-methyltransferase

ptmH knockout mutantはpactamycinの生産が出来なかったことからpactamycin生合成に関与するとしている。培養上清からは新規化合物7-demethyl-7-deoxypactamycinが検出された。このことより、PtmHはhydroxyethyl unitの形成に関与しており、ヒドロキシル化はsecond methylationの後起こると推測された。このORFの機能解析はされていない。

close this sectionSequence

selected fasta
>putative C-methyltransferase [methyltransferase]
MAPLRGRSSPVASEGDLACRLESGGEAGMKTRVLLVQQGVWGNSVASMPLAIGYLKAYAD
ADERIRRRMDISIRNYPGDAGLNAMGRDLIRDGVPDVLCFSVLGWNFRAFGTLAETFKQV
NPDGWVIFGGNHVAHQAERVFRMFPQVDVVVNGEGELVFRDLMNGYLDGARPTALHEISG
VSFREADGNLVTTPERERIQDLEILPSPILTGAIPLADSQGRFLYDYAIMETNRGCPYKC
AFCYWGGATGQKMRAFSRERLREELDVLGRHGAEILMLADSNFGLLRQDEEFLEDLLRVR
AKYGYPNRLETSWAKNKSAGFYRIMEKMKESGMHSAFILALQTMDESVLDLMRRRNMKLN
DWESLVGWLTDHGITPYLELIWGAPGETVESFLDGYDRAARHTPFIAVHPLMLLPNTEYH
DKRQVHGLVTVRGEQDDFDYVLAHRTMTLDDNERMLRFICWNRVLARSLWLHNIWVALRE
LADVPQSRVILSFSDWVESSDDPDARELHALARPTSSASEQVDPHVWRLLTKRLLRKWWD
EAMRPDLPEALLPLLDEVFRYDLMCQPVRMLPDGSGPEEDLPVVEKYGSEWYMRDKVTFT
HPVPELIAALRRGETVSTEPRCHAVTFYYRTQFGGDLQHYFRMDRFRGLTAEQLDHQFTR
V
selected fasta
>putative C-methyltransferase [methyltransferase]
GTGGCCCCATTGCGTGGGAGATCTTCTCCTGTAGCCTCAGAAGGCGACCTGGCCTGTCGG
CTCGAATCAGGGGGCGAAGCAGGAATGAAGACCCGCGTACTACTAGTACAGCAAGGCGTC
TGGGGAAATTCCGTAGCTTCGATGCCGCTGGCGATCGGATACCTCAAAGCCTATGCCGAC
GCCGATGAACGCATCCGGCGACGCATGGACATCTCGATCCGCAACTACCCCGGCGACGCC
GGCCTCAACGCCATGGGCCGGGACCTCATCCGGGACGGGGTGCCCGACGTCCTGTGCTTC
TCCGTGCTGGGCTGGAACTTCCGCGCCTTCGGCACCCTCGCCGAGACGTTCAAGCAGGTC
AACCCGGACGGCTGGGTCATCTTCGGCGGCAACCACGTGGCCCACCAGGCGGAGCGCGTC
TTCCGGATGTTCCCGCAGGTGGACGTGGTGGTGAACGGCGAGGGCGAGCTGGTCTTCCGG
GACCTGATGAACGGCTACCTGGACGGCGCCCGCCCCACCGCGCTGCACGAGATCAGCGGC
GTGTCCTTCCGCGAGGCGGACGGCAACCTGGTCACCACGCCCGAACGCGAGCGCATTCAG
GACCTGGAGATCCTGCCGTCGCCCATTCTCACCGGCGCGATCCCGCTCGCCGACAGCCAG
GGGCGTTTCCTCTACGACTACGCCATCATGGAGACCAACCGCGGCTGCCCCTACAAGTGC
GCCTTCTGTTATTGGGGCGGCGCCACCGGGCAGAAGATGCGCGCCTTCTCCAGGGAACGG
CTCCGCGAGGAACTCGACGTTCTCGGCCGGCACGGTGCGGAAATCCTCATGCTCGCCGAC
TCCAACTTCGGACTCCTGCGCCAGGACGAGGAATTCCTCGAAGACCTCCTCCGGGTGCGT
GCCAAGTACGGCTACCCGAACCGGCTCGAAACCTCCTGGGCCAAGAACAAATCGGCCGGC
TTCTACCGCATCATGGAGAAGATGAAGGAGTCCGGGATGCACAGCGCCTTCATTCTGGCG
CTGCAGACCATGGACGAATCCGTACTGGACCTGATGCGCCGGCGGAACATGAAGCTGAAC
GACTGGGAGAGCCTGGTCGGCTGGCTGACCGATCACGGGATCACCCCTTATCTGGAACTC
ATCTGGGGCGCCCCCGGGGAGACCGTGGAGTCCTTCCTGGACGGTTATGACCGGGCCGCG
CGGCACACCCCGTTCATCGCCGTCCACCCGCTGATGCTGCTGCCCAACACCGAGTACCAC
GACAAGCGGCAGGTGCACGGCCTGGTGACCGTGCGCGGCGAACAGGACGACTTCGACTAC
GTCCTCGCGCACCGGACCATGACGCTCGACGACAACGAGCGCATGCTCCGGTTCATCTGC
TGGAACCGGGTGCTCGCCCGGAGCCTGTGGCTGCACAACATCTGGGTCGCGCTGCGCGAG
CTCGCCGACGTGCCGCAGTCCCGGGTCATCCTCAGCTTCTCCGACTGGGTGGAGAGCAGC
GACGATCCCGACGCCAGGGAGCTGCACGCGCTCGCCCGGCCCACCAGCTCCGCCAGCGAG
CAGGTCGACCCGCACGTCTGGCGGCTGCTCACCAAACGGCTGCTGCGCAAGTGGTGGGAC
GAGGCGATGCGCCCGGACCTGCCCGAGGCGCTGCTGCCGCTGCTGGACGAGGTGTTCCGC
TACGACCTGATGTGCCAGCCGGTGCGGATGCTGCCCGACGGCTCCGGTCCCGAGGAGGAC
CTGCCGGTCGTCGAGAAGTACGGCAGCGAGTGGTACATGCGGGACAAGGTCACCTTCACC
CACCCCGTCCCCGAGCTGATCGCCGCCCTGCGGCGCGGGGAGACGGTGAGCACCGAACCG
CGGTGCCACGCGGTCACCTTCTACTACCGCACGCAGTTCGGCGGCGACCTCCAGCACTAC
TTCCGGATGGACCGCTTCCGGGGGCTGACCGCCGAGCAGCTCGACCACCAGTTCACGCGC
GTTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006158 Cobalamin (vitamin B12)-binding (Domain)
[90-162] 5.10000000000001e-10 PF02310
PF02310   B12-binding
[30-173] PS51332
PS51332   B12_BINDING
IPR006638 Elongator protein 3/MiaB/NifB (Domain)
[226-442] 2.10000267834031e-21 SM00729
SM00729   Elp3
IPR007197 Radical SAM (Domain)
[231-394] 1.3e-14 PF04055
PF04055   Radical_SAM
IPR023404 Radical SAM, alpha/beta horseshoe (Domain)
[226-429] 2.80000000000001e-29 G3DSA:3.80.30.20
G3DSA:3.80.30.20   G3DSA:3.80.30.20
SignalP No significant hit
TMHMM No significant hit
Pact_00100 Pact_00100   Pact_00120 Pact_00120
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