Pact_00140 Pact_00140   Pact_00160 Pact_00160

Pact_00150 : CDS information

close this sectionLocation

Organism
StrainATCC 27456 (=NBRC 13433)
Entry namePactamycin
Contig
Start / Stop / Direction19,030 / 20,757 / + [in whole cluster]
19,030 / 20,757 / + [in contig]
Location19030..20757 [in whole cluster]
19030..20757 [in contig]
TypeCDS
Length1,728 bp (575 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.3 uncharacterized protein (biosynthesis involved)
Producthypothetical protein
Product (GenBank)methyltransferase
GenepctN
ptmL
Gene (GenBank)pctN
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17827660] Cloning of the pactamycin biosynthetic gene cluster and characterization of a crucial glycosyltransferase prior to a unique cyclopentane ring formation. (J Antibiot (Tokyo). , 2007)
[19670201] Deciphering pactamycin biosynthesis and engineered production of new pactamycin analogues. (Chembiochem. , 2009)
[21513878] Biosynthetic studies and genetic engineering of pactamycin analogs with improved selectivity toward malarial parasites. (Chem Biol. , 2011)
comment
[PMID:17827660]
Pactamycin生合成遺伝子クラスターの報告。

PctN: C-methyltransferase

相同性より機能付けされている。
このORFの機能解析はされていない。

シクロペンタン環の修飾過程において、3つのmethyltransferases PctJ(Pact_00110), PctN(このORF), PctO(Pact_00160)によりcyclopentane ringに3つの炭素を付着する反応を行っていると推測している。


[PMID:19670201]
S.pactum ATCC27456のPactamycin生合成遺伝子クラスターの報告。

PtmL: Radical SAM+B12 binding domain

PtmLの機能解析はされていない。

[PMID:21513878]
putative tailoring genesの解析報告(S.pactum ATCC27456)。

PtmL: Radical SAM C-methyltransferases

ptmL knockout mutantはpactamycinあるいはそのanalogsの生産が出来なかったことからpactamycin生合成に関与するとしている。相補実験により機能回復を見ている。PtmLとPtmMがC-6,C-7の付着を触媒するかもしれないと推測している。PtmLの機能解析はされていない。

close this sectionSequence

selected fasta
>hypothetical protein [methyltransferase]
MADAVLLTPREILTGFSSVNNQNVLINDEEYLRLDPAMRLFYEKVRENLGVACIAGHLRA
CGYSVRALNLHGRNPSDEAITDLIRRERPKFVGISIMYDLHIVDAVRLLRCVRKADPSVF
VAIGGAFCTYNAKLIAERIPEADCVAFGEGELTVEGLMECLAAGRDWRSVPGVWFWQEGR
VRSSGPPKLPDLHKQAWPARDLLVHHRGAGIPTPVASTYTSRGCHAKCTFCYVPRAPGVT
AGNAWRVRSPVDVVDEIEFLQREFGTRFLWFNDDNFGGAFQDGYNHAVGFAEEILRRDLK
ISFHCEFRVDTGLIDREALRTLRRAGMASALLGMETGSPAMAKRFRKGTLVEYNFDAARM
FRQENIELEPGWIMVEPGTTVDDLWENLKFIVAADIAVSENPFSFISRAIALRGTEMYDK
ITDPAPPDLAEVEGPAREVLSEARREYRIADGRVEDVWDAWARVSAEVSDRKEELPFVAQ
IIVDATRARRSQGEQGLRPRLSRLRRWVEDLPHLLIAFLNVGLLLADENPPGLAGRLETE
LRALVDAYDREHLGLTYPDFVAETERLCGARALAG
selected fasta
>hypothetical protein [methyltransferase]
ATGGCTGACGCGGTGCTGCTGACGCCACGGGAGATCCTCACCGGGTTCTCCAGCGTCAAC
AACCAGAACGTTCTCATCAACGACGAGGAATACCTCCGGCTGGATCCCGCGATGCGCCTG
TTCTACGAGAAGGTGCGGGAGAACCTGGGGGTGGCGTGCATCGCCGGTCATCTGCGGGCG
TGCGGGTACTCGGTACGGGCGTTGAATCTGCACGGGCGCAACCCCAGCGACGAGGCGATC
ACGGATCTGATCCGCCGCGAGCGGCCGAAGTTCGTGGGCATCAGCATCATGTACGACCTG
CACATCGTGGACGCGGTGCGGCTGCTGCGCTGCGTGCGCAAGGCCGACCCGTCGGTGTTC
GTGGCGATCGGCGGGGCGTTCTGCACGTACAACGCCAAACTGATCGCCGAGCGCATCCCG
GAGGCGGACTGCGTGGCCTTCGGCGAGGGCGAGCTGACCGTCGAGGGGCTGATGGAGTGC
CTGGCCGCCGGCCGCGACTGGCGGTCGGTGCCCGGCGTGTGGTTCTGGCAGGAAGGACGG
GTGCGCAGCAGCGGCCCGCCGAAGCTGCCGGACCTGCACAAGCAGGCCTGGCCCGCCCGC
GACCTGCTCGTCCACCACCGCGGGGCCGGCATCCCCACCCCGGTGGCGTCCACGTACACC
AGCCGCGGCTGCCACGCCAAGTGCACCTTCTGCTACGTGCCGCGGGCCCCCGGCGTCACC
GCCGGCAACGCCTGGCGGGTGCGGTCGCCCGTCGACGTGGTGGACGAGATCGAGTTCCTC
CAGCGGGAGTTCGGCACCCGGTTCCTGTGGTTCAACGACGACAACTTCGGCGGCGCCTTC
CAGGACGGCTACAACCACGCCGTGGGCTTCGCCGAGGAGATCCTGCGCCGTGATCTGAAG
ATCTCCTTCCACTGCGAGTTCCGGGTGGACACCGGGCTGATCGACCGGGAGGCGCTGCGC
ACCCTGCGCCGGGCCGGCATGGCCTCGGCGCTGCTGGGCATGGAGACCGGCTCCCCGGCG
ATGGCCAAACGGTTCCGCAAGGGCACCCTGGTCGAGTACAACTTCGACGCCGCGCGGATG
TTCCGCCAGGAGAACATCGAGCTGGAACCCGGCTGGATCATGGTCGAGCCCGGCACCACC
GTGGACGACCTGTGGGAGAACCTGAAGTTCATCGTGGCGGCCGACATCGCCGTCAGCGAG
AACCCGTTCTCCTTCATCAGCCGGGCCATCGCGCTGCGCGGCACCGAGATGTACGACAAG
ATCACCGATCCGGCGCCGCCGGACCTGGCGGAGGTCGAGGGCCCCGCGCGGGAGGTGCTG
AGCGAGGCGCGCCGGGAGTACCGGATCGCCGACGGCCGGGTCGAGGACGTGTGGGACGCC
TGGGCCAGGGTGAGCGCCGAGGTCAGCGACCGCAAGGAGGAGCTGCCCTTCGTCGCCCAG
ATCATCGTGGACGCCACCCGGGCCCGCCGCTCCCAGGGCGAGCAGGGCCTGCGTCCGCGT
CTGAGCCGGCTGCGGCGCTGGGTCGAGGACCTGCCGCACCTGCTGATCGCCTTCCTCAAC
GTCGGACTGCTGCTCGCGGACGAGAACCCGCCGGGTCTCGCCGGCCGGCTGGAGACCGAA
CTGCGCGCGCTGGTCGATGCCTACGACCGCGAGCACCTGGGGCTCACCTACCCGGACTTC
GTGGCGGAGACCGAGCGTTTGTGCGGAGCACGGGCCCTGGCCGGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006158 Cobalamin (vitamin B12)-binding (Domain)
[47-157] 1.1e-20 PF02310
PF02310   B12-binding
[49-187] 6.09999977126211e-12 SSF52242
SSF52242   Cbl-bd
[50-146] 1.2e-06 G3DSA:3.40.50.280
G3DSA:3.40.50.280   B12_bd
[31-168] PS51332
PS51332   B12_BINDING
IPR006638 Elongator protein 3/MiaB/NifB (Domain)
[214-438] 1.29999924468179e-21 SM00729
SM00729   Elp3
IPR007197 Radical SAM (Domain)
[219-391] 1.6e-13 PF04055
PF04055   Radical_SAM
IPR023404 Radical SAM, alpha/beta horseshoe (Domain)
[215-426] 1.99999999999999e-31 G3DSA:3.80.30.20
G3DSA:3.80.30.20   G3DSA:3.80.30.20
SignalP No significant hit
TMHMM No significant hit
Pact_00140 Pact_00140   Pact_00160 Pact_00160
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