Pladi_00050 : CDS information

Location

Organism	Streptomyces platensis
Strain	Mer-11107
Entry name	Pladienolide
Contig	AB435553
Start / Stop / Direction	65,707 / 66,906 / + [in whole cluster] 65,707 / 66,906 / + [in contig]
Location	65707..66906 [in whole cluster] 65707..66906 [in contig]
Type	CDS
Length	1,200 bp (399 aa)

Annotation

Category	3.3 modification reduction
Product	cytochrome P450
Product (GenBank)	cytochrome P450
Gene
Gene (GenBank)	pldB
EC number
Keyword	C-6 hydroxylate
Note
Note (GenBank)
Reference	ACC B6ZIR6 PmId [18997414] Organization of the biosynthetic gene cluster for the polyketide antitumor macrolide, pladienolide, in Streptomyces platensis Mer-11107. (Biosci Biotechnol Biochem. , 2008) comment Streptomyces platensis Mer-11107におけるpladienolide biosynthetic gene clusterに関する文献。 PldB(399 aa): C6-hydroxylase (Cytochrome P450) pldB不活化変異株では、pladienolide Bは検出されず、6-deoxy pladienolide Bのみが検出されたことが記されている。また、PldBの酵素活性を調べており、6-deoxy pladienolide Bがpladienolide Bに変換されることが確認されており、pldB遺伝子は6-deoxy pladienolide B 6-hydroxylaseをエンコードしていると結論付けている。

Sequence

>cytochrome P450 [cytochrome P450]
MDLETQLLSPAYLRNPHPLNAALRSADPVQRAVASGGLSVWVVTRYEDVRALLADSRLGK
GVTQLREAVLLNAGDDERISQFTDSLTEHMLNSDPPDHTRLRRLVGKAFTAGRIEQLRPR
ITEIVDNLLDRLSPGQEVDLVPVFALPMPTTVICELLGVPSVDRSSFSHWSNVLVSTAEV
GELAEAGGAMVAYLAQLIADKRANPCDDLLTKLVQATDNGDQLSETELVATAFLLLSAGH
ETTVNLIAAGTLTLLQNPDQLARLRSDLTLLPGAIEELIRYDGPGGMVLRHTLEPVEVGG
VTIPAQQVVLLSLSSAGRDSTRFSDADRLDIGRPIGGSVGFGHGIHHCIGAPLARLEGEI
AFRALLTRFPDLRLAVPPEELNWRDSVFIRGPESLPVVL

>cytochrome P450 [cytochrome P450]
ATGGATCTGGAAACCCAACTTCTCTCCCCGGCATACCTACGGAACCCGCACCCGCTCAAC
GCCGCATTGCGTTCCGCCGACCCTGTTCAACGTGCCGTGGCTTCGGGGGGCCTGTCCGTC
TGGGTGGTGACCCGCTACGAGGACGTGCGCGCGCTGCTCGCCGATTCCAGGCTGGGCAAA
GGCGTCACGCAGCTCCGCGAGGCGGTACTGCTCAACGCGGGTGACGACGAGCGGATCAGC
CAGTTCACCGACTCCCTCACCGAGCACATGCTCAACAGCGACCCACCCGACCACACCCGG
CTGCGCCGCCTGGTCGGCAAGGCGTTCACCGCCGGCCGCATAGAACAGCTTCGCCCCAGG
ATCACGGAGATCGTCGACAATCTACTGGACCGGCTGAGTCCCGGTCAGGAGGTCGACCTC
GTCCCTGTCTTCGCCCTGCCCATGCCGACCACTGTGATCTGCGAACTGCTCGGCGTGCCG
TCCGTCGACCGGTCGTCGTTCAGCCACTGGTCCAATGTGCTGGTGTCGACCGCGGAAGTC
GGCGAACTGGCCGAGGCCGGCGGAGCGATGGTCGCCTATCTGGCACAGCTCATCGCGGAC
AAACGCGCCAACCCCTGTGACGACCTGCTCACCAAGCTGGTGCAAGCCACCGACAACGGC
GACCAGCTCTCCGAGACGGAACTCGTGGCGACGGCCTTCCTGCTGCTGTCCGCCGGGCAC
GAGACCACGGTGAACCTCATTGCCGCCGGTACGCTCACTCTGCTCCAGAACCCGGACCAG
CTCGCCCGGTTGCGCTCCGACCTCACGCTGCTGCCCGGCGCGATCGAGGAGCTCATACGG
TACGACGGGCCCGGCGGCATGGTGCTCCGGCACACCCTGGAGCCGGTCGAGGTCGGCGGT
GTGACCATCCCGGCCCAGCAGGTCGTCCTGCTCTCGCTGTCCTCGGCGGGCCGCGACTCC
ACCCGGTTCAGCGACGCCGACCGGCTCGACATCGGCCGTCCCATCGGGGGCAGCGTGGGG
TTCGGGCACGGTATCCACCACTGCATCGGCGCCCCGCTCGCCAGGCTGGAGGGCGAGATC
GCGTTCCGGGCCCTGCTCACCCGCTTCCCCGACCTGCGGCTCGCGGTCCCGCCGGAGGAG
CTGAACTGGCGCGACAGTGTCTTCATCCGCGGCCCGGAATCGCTGCCCGTGGTGCTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 SSF48264 Cytochrome_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Pladi_00040 Pladi_00060