Pladi_00060 : CDS information

Location

Organism	Streptomyces platensis
Strain	Mer-11107
Entry name	Pladienolide
Contig	AB435553
Start / Stop / Direction	68,160 / 66,967 / - [in whole cluster] 68,160 / 66,967 / - [in contig]
Location	complement(66967..68160) [in whole cluster] complement(66967..68160) [in contig]
Type	CDS
Length	1,194 bp (397 aa)

Annotation

Category	3.4 other modification
Product	putative acyltransferase
Product (GenBank)	putative acyltransferase
Gene
Gene (GenBank)	pldC
EC number
Keyword	C-7
Note
Note (GenBank)
Reference	ACC B6ZIR7 PmId [18997414] Organization of the biosynthetic gene cluster for the polyketide antitumor macrolide, pladienolide, in Streptomyces platensis Mer-11107. (Biosci Biotechnol Biochem. , 2008) comment Streptomyces platensis Mer-11107におけるpladienolide biosynthetic gene clusterに関する文献。 PldC(397 aa): putative acyltransferase pldC自体の機能実験は行われておらず、相同性よりpladienolide 7-O-acetylationとして機能するCpA-dependent acyltransferase familyに属する酵素であると予想している。
Related Reference	ACC P96208 PmId [15070765] Mycobacterial polyketide-associated proteins are acyltransferases: proof of principle with Mycobacterium tuberculosis PapA5. (Proc Natl Acad Sci U S A. , 2004) comment <Phthiocerol/phthiodiolone dimycocerosyl transferase (papA5), Mycobacterium tuberculosis, BLAST 18; id=27%> Mycobacterium tuberculosis のPapA5がacetyltransferaseであることをpapA5のリコンビナントタンパク質を使って示した文献。

Sequence

>putative acyltransferase [putative acyltransferase]
MALCTVRGDTNEQLLQRAFASSVAAHPSLRSRISPDGTELVLHPLDDGPPELVVRRAGSW
DLDREMRSRLDRCGPLVRATLLRGAAEDTFILHVDHRICDGRSVVALLSAVWRTYAALGE
GPMASSAHVADSYPAPIETRLGHHPEADVLAYAARRAEQAKRLPPVLLPYLGDPGVEAPE
QGEIHVRTLRLTSDETTRLAGSARAAGISVQGLVAAALLIAVRRALEATDAPLSLALASP
VDFRHRVTPPLAEETLVLAAASFYDIVEVSPRADVRTLGRLVYDRLRAGVERGDPEREIL
AVRHFFENPALLAASLVLTNLGRVADLVAPPGLELGGLRWIPVPENWSPEQGRGPLVVSA
ITVEGRLALEVPYSPSCFGHRQIAEVVESTRRILMSA

>putative acyltransferase [putative acyltransferase]
ATGGCGCTGTGCACGGTACGGGGCGATACCAACGAACAGCTTCTCCAACGGGCCTTCGCC
TCGTCCGTGGCCGCGCACCCCTCCTTGCGGAGCCGGATATCGCCGGACGGTACCGAGTTG
GTGCTGCACCCGCTGGACGACGGGCCGCCCGAACTCGTCGTCCGCCGGGCCGGCTCCTGG
GACCTCGACCGGGAGATGCGGAGCCGGCTGGACCGCTGCGGCCCGCTGGTCCGCGCCACC
CTGCTGCGCGGCGCGGCGGAGGACACCTTCATACTCCATGTCGATCACCGGATCTGCGAC
GGCCGCAGCGTGGTCGCCTTGCTGTCGGCGGTGTGGCGGACGTACGCCGCGCTGGGCGAA
GGGCCGATGGCGTCCTCGGCACACGTGGCCGACAGCTACCCGGCGCCCATCGAGACCCGT
CTCGGCCATCACCCGGAGGCGGATGTCCTCGCCTACGCGGCCCGCCGGGCCGAGCAGGCG
AAGCGCCTTCCCCCCGTGCTGCTGCCCTATCTGGGGGACCCGGGCGTGGAAGCGCCGGAG
CAGGGCGAGATACACGTAAGGACCCTCCGGCTCACTTCCGACGAGACGACGCGGCTCGCC
GGATCCGCCCGGGCCGCCGGGATCTCGGTACAGGGCCTGGTGGCGGCGGCACTGCTCATC
GCGGTGCGCCGCGCCCTCGAAGCGACCGACGCACCCCTTTCGCTGGCCTTGGCGTCCCCC
GTCGACTTCCGGCACCGGGTCACTCCACCGCTGGCAGAGGAGACCCTGGTGCTGGCCGCC
GCGTCGTTCTACGACATTGTCGAAGTCTCCCCGCGGGCGGATGTGCGCACGCTCGGCCGG
CTGGTCTACGACCGGCTTCGCGCGGGTGTCGAGCGGGGCGATCCCGAACGCGAGATCCTC
GCGGTGCGCCACTTCTTTGAGAATCCCGCTCTGCTGGCGGCCAGTCTCGTGCTCACCAAC
CTGGGCCGTGTCGCGGACCTGGTGGCACCCCCCGGGCTCGAGCTGGGCGGTTTGCGGTGG
ATCCCGGTACCGGAGAACTGGTCGCCGGAGCAGGGGCGGGGGCCGCTGGTGGTGTCGGCG
ATCACCGTGGAGGGCCGGCTGGCCCTGGAAGTCCCGTATTCGCCCAGCTGCTTCGGGCAT
CGGCAGATCGCCGAGGTAGTCGAAAGCACCCGTCGGATCCTCATGTCGGCGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001242 Condensation domain (Domain) PF00668 Condensation
SignalP	No significant hit
TMHMM	No significant hit

Pladi_00050 Pladi_00070