Pladi_00070 : CDS information

Location

Organism	Streptomyces platensis
Strain	Mer-11107
Entry name	Pladienolide
Contig	AB435553
Start / Stop / Direction	69,661 / 68,267 / - [in whole cluster] 69,661 / 68,267 / - [in contig]
Location	complement(68267..69661) [in whole cluster] complement(68267..69661) [in contig]
Type	CDS
Length	1,395 bp (464 aa)

Annotation

Category	3.3 modification reduction
Product	putative epoxidase
Product (GenBank)	putative epoxidase
Gene
Gene (GenBank)	pldD
EC number
Keyword
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference	ACC B6ZIR8 PmId [18997414] Organization of the biosynthetic gene cluster for the polyketide antitumor macrolide, pladienolide, in Streptomyces platensis Mer-11107. (Biosci Biotechnol Biochem. , 2008) comment Streptomyces platensis Mer-11107におけるpladienolide biosynthetic gene clusterに関する文献。 PldD(433 aa): putative epoxidase pldD 自体の機能実験は行われておらず、monCI(monesin epoxidase)との相同性より18, 19-epoxydaseとして機能すると予想している。
Related Reference	ACC Q846W9 NITE Monen_00220 PmId [12940979] Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. (Mol Microbiol. , 2003) comment <Monensin epoxidase(monC1), Streptomyces cinnamonensis, BLAST 11; id=40%> Streptomyces cinnamonensisのmonensin biosynthetic gene clusterにおけるmonB と monCの機能について調べた文献。 monCI について、S. coelicolorにおけるHeterologous expressionを行ったところ、linaloolをlinalool oxideにepoxidizeする能力が10-20倍に増加することを確認している。

Sequence

>putative epoxidase [putative epoxidase]
MDMVNRAIVLGSGLAGALTASVLAKFVDEVVMLDRDQVPDGPEVRKGTPQTLHSHILMSN
GARTIDSLVPGSLHRLLAAGAHRTEVPSGLVSCSRQGWARRMPGAQFMVTCGRPLLDWTL
RRLVLEDDRITLRSGVDVQGLDGDATRVTGVQAQDRASGESLRLDADFVVDATGRGSGAN
TWLQALGLPAVREVKIDIGLSYATRRYRAPAGAESGFPIVNVLPDPEDDQPGQGAVLLPI
EDGQWIVTLTGTRGCEPPRDPEGFVAFARRLRHSVIGDLIANAEPIGPIHSSRTTVNRRR
YYEELADWPKGFVVLGDAAAALNPVYGHGMSVAAMSASALRDVLRSDGLVAGTSRATQAA
VAGAVNNAWAMATGQDIFYPNVSGRRPGLAARMQRRYVNRVTKTAADRPRVAAAVSDTFT
LSAPLTRLMTPRIVFETLLGPTRPPLTGPPLTSRERESIVGSPQ

>putative epoxidase [putative epoxidase]
ATGGATATGGTGAATCGCGCAATAGTTCTCGGCAGCGGTCTGGCTGGTGCCCTTACGGCA
TCCGTGCTCGCGAAGTTCGTGGACGAGGTAGTGATGCTGGACCGGGACCAGGTACCGGAC
GGCCCGGAAGTCAGAAAGGGCACCCCACAGACCCTCCACTCGCACATCCTGATGTCGAAC
GGCGCACGGACCATCGACTCGCTGGTGCCCGGGAGCCTCCACCGCCTGCTGGCCGCCGGT
GCGCACCGGACCGAGGTACCCAGCGGGCTCGTCTCCTGCTCACGGCAGGGGTGGGCCCGA
AGGATGCCCGGCGCCCAGTTCATGGTCACCTGTGGTCGTCCGCTGCTGGACTGGACGCTG
CGGCGCCTCGTCCTGGAAGACGACCGCATCACCCTGCGGAGCGGTGTTGACGTCCAGGGG
CTCGACGGCGATGCCACCCGGGTGACCGGGGTCCAGGCCCAGGACCGGGCGTCCGGGGAA
TCGCTCCGGCTGGACGCGGACTTCGTGGTGGACGCCACCGGCCGTGGATCCGGCGCGAAC
ACCTGGCTCCAGGCGCTCGGTCTGCCCGCCGTGCGCGAGGTGAAGATCGACATCGGGCTC
TCCTACGCCACCCGCCGGTACCGCGCGCCGGCCGGGGCGGAGAGCGGCTTTCCCATCGTG
AACGTGCTGCCGGACCCGGAGGACGACCAGCCGGGCCAGGGCGCGGTGCTGCTGCCGATC
GAGGACGGACAGTGGATCGTCACGCTGACCGGGACCCGTGGGTGCGAGCCGCCGCGTGAT
CCGGAAGGCTTCGTGGCGTTCGCCCGGAGACTGCGGCATTCGGTGATCGGGGACCTCATC
GCGAACGCCGAGCCGATCGGGCCGATCCACAGCAGTCGCACCACGGTCAACCGCCGCCGC
TACTACGAGGAACTGGCCGACTGGCCGAAGGGCTTCGTGGTGCTCGGCGACGCCGCGGCC
GCCCTGAACCCGGTGTACGGGCACGGCATGTCGGTGGCCGCCATGAGCGCTTCGGCGCTG
CGCGACGTCCTGCGGAGCGACGGGTTGGTGGCCGGGACGAGCCGCGCCACACAGGCCGCC
GTCGCGGGCGCCGTCAACAATGCGTGGGCGATGGCCACAGGACAGGACATCTTCTACCCG
AATGTGAGTGGCCGGCGGCCGGGGCTGGCCGCGCGGATGCAGCGGCGCTATGTCAACCGG
GTGACCAAGACCGCAGCCGACCGCCCCCGGGTGGCCGCCGCGGTGTCGGACACGTTCACC
CTGTCGGCCCCGCTGACCCGGCTGATGACGCCCCGGATCGTCTTCGAGACCCTTCTCGGC
CCTACCCGGCCGCCGCTCACCGGGCCCCCTCTCACCTCCCGGGAACGGGAATCCATCGTC
GGCTCGCCCCAGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	Transmembrane 1

Pladi_00060 Pladi_00080