Polk_00140 : CDS information

Location

Organism	Streptomyces diastatochromogenes
Strain	Tü6028
Entry name	Polyketomycin
Contig	FJ483966
Start / Stop / Direction	16,772 / 15,342 / - [in whole cluster] 16,772 / 15,342 / - [in contig]
Location	complement(15342..16772) [in whole cluster] complement(15342..16772) [in contig]
Type	CDS
Length	1,431 bp (476 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	PokS3
Gene
Gene (GenBank)	pokS3
EC number
Keyword	beta-D-amicetose alpha-L-axenose
Note
Note (GenBank)	putative dTDP-hexose 2,3-dehydratase
Reference	ACC C0JWA9 PmId [19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009) comment polyketomycin生合成clusterの解析論文。機能推定は配列解析から。 pokS3: dTDP-hexose 2,3-dehydratase
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment Blast 19th, id52%, 1e-127 Saccharopolyspora spinosa_spnO Probable NDP-hexose-2,3-dehydratase Forosamine合成の初期段階で NDP-4-keto-6-deoxy-glucoseの2,3位の脱水反応を行う。 dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [PokS3]
MTTERTLTAGGPGPQARFTESGAAAEDRASVKATLAWLTARREAHTFEVTRVPFAELAEW
SFRPDTGDLGHRSGRFFTVEGHRVHTDHGKVPRWDQPIINQPEIGMLGFLVKEIDGVLHC
LMQAKMEPGNVNVVQLSPTVQATRSNQKRVHQGSGVRYLEYFTDPGRARVLVDVLQSEQG
SWFLGKRNRNIVVETTEDVPPHDDYRWMTIGEIQELLRLDNMVNMDARTVLSCVPFARPT
GAPPPGPHGEFRAGLRRSMAPEEGALHSTPSVLSWFADTTARHELRTERIPLNRVEGWNR
TADAISHAEGRHFSIIGARVTAGSREVLNWTQPLLAPHGLGLSALLVRTIGDVTHLLFHA
RPEAGFPHGVELGPTVQCTPDTYRGLPDDHRPPFLDYVTGVPRDRVRFDAVQSEEGGRFH
HAECRYTVIEVEDGFPVEEPEGYRWLTVHQVTGLLRHSHYLNVQARSLVACLHSLW

>putative NDP-hexose 2,3-dehydratase [PokS3]
ATGACCACCGAGCGCACACTCACAGCCGGCGGACCCGGTCCGCAGGCGCGGTTCACCGAG
TCGGGAGCCGCGGCCGAGGACCGGGCGTCGGTCAAGGCCACGCTCGCCTGGCTGACCGCA
CGCAGGGAGGCCCACACCTTCGAGGTCACCCGGGTCCCCTTCGCCGAGCTGGCGGAGTGG
TCCTTCCGGCCTGACACCGGCGATCTCGGCCACCGCAGCGGCCGGTTCTTCACGGTCGAG
GGCCATCGCGTGCACACCGACCACGGGAAGGTGCCCCGCTGGGACCAGCCGATCATCAAC
CAGCCCGAGATCGGCATGCTCGGCTTCCTGGTCAAGGAGATCGACGGCGTCCTGCACTGC
CTGATGCAGGCCAAGATGGAGCCCGGCAACGTCAACGTCGTACAGCTGTCACCGACCGTG
CAGGCGACCCGGAGCAACCAGAAGCGGGTGCACCAGGGTTCGGGCGTCAGGTACCTGGAG
TACTTCACCGATCCCGGGCGGGCACGGGTGCTCGTCGACGTCCTCCAGTCCGAACAGGGC
TCGTGGTTCCTCGGCAAGCGCAACCGCAACATCGTGGTGGAGACGACCGAGGACGTCCCG
CCGCACGACGACTACCGCTGGATGACGATCGGCGAGATCCAGGAACTCCTGCGCCTGGAC
AACATGGTGAACATGGACGCCAGGACCGTCCTGTCCTGTGTGCCGTTCGCCCGGCCCACG
GGAGCGCCGCCGCCCGGTCCGCACGGCGAGTTCCGCGCGGGCCTGCGCCGCTCCATGGCG
CCGGAGGAGGGGGCGCTGCACAGCACGCCGTCGGTGCTGAGCTGGTTCGCCGACACCACG
GCCCGCCACGAACTGCGCACGGAACGCATCCCGTTGAACCGCGTCGAGGGCTGGAACCGC
ACCGCGGACGCGATCAGCCATGCGGAGGGACGGCACTTCTCCATCATCGGCGCCCGGGTG
ACGGCCGGCAGCCGCGAGGTGCTCAACTGGACGCAGCCGCTACTGGCCCCGCACGGCCTG
GGCCTGTCCGCACTGCTGGTGCGCACCATCGGCGACGTGACCCATCTGCTGTTCCACGCC
CGTCCCGAGGCGGGATTCCCGCACGGCGTCGAACTGGGCCCCACCGTGCAGTGCACCCCG
GACACCTACCGGGGGCTGCCCGACGACCACCGGCCGCCGTTCCTCGACTACGTGACCGGC
GTCCCCCGCGACCGGGTGCGCTTCGACGCGGTGCAGTCCGAGGAGGGCGGCCGATTCCAC
CACGCCGAGTGCCGTTACACGGTGATCGAGGTCGAGGACGGCTTCCCCGTCGAAGAACCG
GAGGGATACCGATGGTTGACGGTCCATCAGGTGACCGGGCTGCTGCGGCACAGTCACTAT
CTCAACGTCCAGGCGCGCAGCCTCGTCGCATGTCTGCACAGTCTGTGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Polk_00130 Polk_00150