Polk_00360 Polk_00360   Polk_00380 Polk_00380

Polk_00370 : CDS information

close this sectionLocation

Organism
StrainTü6028
Entry namePolyketomycin
Contig
Start / Stop / Direction45,302 / 44,547 / - [in whole cluster]
45,302 / 44,547 / - [in contig]
Locationcomplement(44547..45302) [in whole cluster]
complement(44547..45302) [in contig]
TypeCDS
Length756 bp (251 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase
Product (GenBank)PokT2
Gene
Gene (GenBank)pokT2
EC number
Keyword
Note
Note (GenBank)
  • putative ketoreductase
Reference
ACC
PmId
[19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009)
comment
polyketomycin生合成clusterの解析論文。

機能推定は配列解析から。
pokT2: ketoreductase
Related Reference
ACC
P50205
PmId
[7582015] ( , )
comment
Blast 41st, id40%, 1e-31
Rhizobium meliloti_phbB
Acetoacetyl-CoA reductase(EC 1.1.1.36)

(R)-3-hydroxyacyl-CoA + NADP+ = 3-oxoacyl-CoA + NADPH.

E. coli に3genes; beta-ketothiolase (phaA, phbA), acetoacetyl-CoA reductase (phaB, phbB), PHB-synthase (phaC, phbC)の導入でpolyhydroxyalkanoate生成。

close this sectionSequence

selected fasta
>putative oxidoreductase [PokT2]
MDLGLTDRVVLVTGGTRGIGEATVRAFAAEGARVALTYAGAKDTADRLVRELGGPDRALA
VHYDLARPGSVEDLVPTVERHFGTVDVLVANAVRRPAGRPRPGFERLDPADWAAFLDDNL
TGTIRTVQTALGAMRAQGRGRIALISSHLAAKGQAGQEVYTAAKSALHGFALSLARDAGP
DGVLVNVISPGLTTTEGALSHLPAAVLEKAAAALPTGRLGSPEEVARTVVFYCSAANENI
TGELVSLVGRG
selected fasta
>putative oxidoreductase [PokT2]
ATGGATCTCGGACTGACCGACCGGGTCGTCCTCGTCACGGGCGGCACCCGGGGCATCGGG
GAGGCGACCGTGCGCGCCTTCGCCGCCGAGGGCGCCCGCGTCGCGCTGACCTATGCCGGC
GCGAAGGACACAGCGGACCGCCTGGTGCGCGAACTCGGCGGACCGGACAGGGCTCTGGCG
GTCCACTACGACCTGGCCCGGCCCGGCTCGGTCGAGGACCTCGTACCGACGGTCGAGCGG
CACTTCGGCACGGTCGACGTCCTCGTCGCGAACGCCGTGCGCCGGCCCGCGGGACGCCCG
CGGCCGGGCTTCGAACGCCTGGACCCGGCGGACTGGGCCGCGTTCCTGGACGACAACCTG
ACCGGGACGATCCGCACGGTGCAGACCGCGCTCGGCGCCATGCGTGCCCAGGGCCGCGGC
CGCATCGCCCTGATCTCCTCCCACCTGGCCGCCAAGGGCCAGGCGGGCCAGGAGGTCTAC
ACGGCGGCGAAGTCGGCGCTGCACGGCTTCGCTCTCAGTCTGGCCCGCGACGCCGGCCCC
GACGGGGTCCTGGTCAATGTGATCAGTCCCGGGCTCACCACGACCGAGGGGGCCTTGAGC
CACCTCCCCGCGGCCGTCCTGGAGAAGGCCGCGGCCGCACTGCCGACCGGGCGCCTCGGC
AGCCCGGAGGAGGTCGCCAGGACCGTCGTCTTCTACTGCTCGGCGGCCAACGAGAACATC
ACCGGCGAGCTGGTCTCCCTGGTCGGGCGCGGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[83-94] 2.90000036269908e-05 PR00080 [140-148] 2.90000036269908e-05 PR00080 [160-179] 2.90000036269908e-05 PR00080
PR00080   SDRFAMILY
[9-177] 1.1e-27 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[9-26] 1.20000117458134e-32 PR00081 [83-94] 1.20000117458134e-32 PR00081 [134-150] 1.20000117458134e-32 PR00081 [160-179] 1.20000117458134e-32 PR00081 [181-198] 1.20000117458134e-32 PR00081 [215-235] 1.20000117458134e-32 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[4-247] 3.19999999999996e-67 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[147-175] PS00061
PS00061   ADH_SHORT
SignalP
[1-28] 0.103 Signal
Bacteria, Gram-negative   
[1-28] 0.167 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Polk_00360 Polk_00360   Polk_00380 Polk_00380
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