Pyrlo_00110 : CDS information

Location

Organism	Actinosporangium vitaminophilum
Strain	ATCC 31673 (=NBRC 14294)
Entry name	Pyrrolomycin
Contig	EF140901
Start / Stop / Direction	13,560 / 11,887 / - [in whole cluster] 13,560 / 11,887 / - [in contig]
Location	complement(11887..13560) [in whole cluster] complement(11887..13560) [in contig]
Type	CDS
Length	1,674 bp (557 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	halogenase
Gene
Gene (GenBank)	pyr11
EC number
Keyword
Note	Similar to halogenases but no obvious NAD(P)H binding region.
Note (GenBank)
Reference	ACC A3R4Q2 PmId [17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007) comment pyrrolomycin biosynthetic gene clustersに関する文献。 pyr11(557 a.a.): halogenase(?); FADH2 motif absent pyr11自体の機能実験は行われていないが、相同性よりhalogenaseと予測している。しかし、通常のhalogenaseに存在する2つのmotifが保存されていないので、halogenaseとしては機能しないのではないかと記されている。

Sequence

>hypothetical protein [halogenase]
MKAIKSPEHDRRLARAADPAEKYDVAILGGSMAAGLLGAVLSRQGVRVLLVGAADDDSDP
AGETTVPYTAEVFLLLAKRFQVPEIAAFGLFTDLPPWVRSESGVKKSLGFLYHHPGRPQD
PHECVQFNVPNEHGEWHLYRRGVDRYTRELAAKYGAALAGADVFVSDAWVEEDEGRVRVS
DGTVYRARFLVDCVGPDSPLLVRNGGDDAEPRLRHTSRVYATQMRGVVPFEALVPPSRQD
KVTPWSEGTVHHLFDGGWVQLVDFGNHKESRNPTTSVTLSVDPERFPDLPEEPDKAFRQV
VERFPDLARQFENATPVRPWTVETRYQRTASTTHGERWFGLERTAARNDMFLARDATMAA
ESVHALASVLIPAVRRDNWSPAPFARVALFQEALGEFNDRLLHAARTACQDFRLWNAFSR
VWLLWQILADLSLKRARLDAESSGDWSVCEQYELGGIWFQCPRGLRELIDRSLETVDEVR
RGGLAAGAAADRIFAELRREPFVPPLYAFGDPGARVYRFTLPKRLQMLFWVKTKAPADFR
RLLTVDNVSGVTAASSR

>hypothetical protein [halogenase]
ATGAAAGCCATCAAGTCCCCCGAGCACGACCGGCGGCTCGCCAGGGCGGCCGACCCCGCC
GAGAAGTACGACGTCGCGATACTCGGCGGCTCCATGGCCGCCGGGCTGCTGGGAGCGGTG
CTGTCCCGGCAGGGAGTGCGGGTGCTGTTGGTCGGCGCGGCCGACGACGACAGTGACCCC
GCCGGGGAGACCACGGTCCCCTACACGGCCGAGGTCTTCCTCCTGTTGGCCAAGCGCTTC
CAGGTGCCGGAGATCGCGGCCTTCGGGTTGTTCACCGACCTCCCGCCCTGGGTCAGGAGC
GAGAGCGGGGTCAAGAAGAGCCTGGGCTTCCTCTACCACCACCCGGGACGTCCCCAGGAC
CCCCACGAGTGCGTCCAGTTCAACGTCCCCAACGAGCACGGCGAATGGCACCTGTACCGG
CGGGGCGTGGACCGTTACACCCGGGAGCTGGCCGCGAAGTACGGGGCCGCGCTCGCCGGT
GCGGACGTGTTCGTCTCCGACGCCTGGGTGGAGGAGGACGAGGGCCGGGTGCGGGTCAGC
GACGGCACCGTCTACCGGGCCCGGTTCCTGGTGGACTGCGTCGGTCCCGACTCGCCGCTG
CTGGTGCGCAACGGGGGGGACGACGCCGAGCCCCGGCTGCGGCACACCTCGCGGGTCTAC
GCCACCCAGATGCGGGGGGTCGTCCCGTTCGAGGCCCTGGTGCCGCCGAGCCGCCAGGAC
AAGGTCACCCCGTGGTCCGAGGGCACGGTGCACCACCTGTTCGACGGCGGCTGGGTCCAG
TTGGTGGACTTCGGCAACCACAAGGAGAGCCGCAACCCGACCACCAGCGTCACCCTCAGC
GTCGACCCGGAGCGCTTCCCCGACCTGCCCGAGGAGCCGGACAAGGCCTTCCGCCAGGTG
GTGGAGAGGTTCCCGGACCTCGCCAGGCAGTTCGAGAACGCCACGCCGGTGCGTCCCTGG
ACCGTGGAGACCCGCTACCAGCGCACCGCGAGCACCACCCACGGCGAGCGTTGGTTCGGC
CTGGAGCGCACGGCCGCCCGCAACGACATGTTCCTGGCCCGGGACGCGACCATGGCCGCC
GAGTCGGTGCACGCCCTGGCCTCGGTGCTGATCCCCGCGGTGCGCCGGGACAACTGGTCG
CCCGCTCCGTTCGCGCGCGTGGCCCTGTTCCAGGAAGCCCTCGGCGAGTTCAACGACCGG
CTGCTGCACGCCGCCAGGACCGCCTGCCAGGACTTCCGGCTGTGGAACGCCTTCTCCCGG
GTGTGGCTGCTGTGGCAGATCCTGGCCGACCTGTCGCTGAAGCGGGCCCGGCTGGACGCC
GAGTCCAGCGGGGACTGGTCGGTGTGCGAGCAGTACGAACTGGGCGGGATCTGGTTCCAG
TGCCCGCGGGGCCTGCGGGAGCTGATCGACCGCAGCCTGGAGACCGTGGACGAGGTGCGC
CGCGGGGGACTGGCGGCCGGGGCCGCGGCGGACCGCATCTTCGCGGAACTGCGCCGGGAG
CCGTTCGTGCCGCCGCTGTACGCGTTCGGGGACCCGGGCGCCCGCGTGTACCGGTTCACC
CTGCCCAAGCGGTTGCAGATGCTGTTCTGGGTGAAGACCAAGGCTCCCGCGGACTTCCGC
AGGCTGCTGACGGTGGACAACGTCAGCGGGGTGACGGCTGCCTCGAGCCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Eukaryota Bacteria, Gram-negative
TMHMM	No significant hit

Pyrlo_00100 Pyrlo_00120