Pyrlo_00220 : CDS information

Location

Organism	Actinosporangium vitaminophilum
Strain	ATCC 31673 (=NBRC 14294)
Entry name	Pyrrolomycin
Contig	EF140901
Start / Stop / Direction	29,323 / 28,625 / - [in whole cluster] 29,323 / 28,625 / - [in contig]
Location	complement(28625..29323) [in whole cluster] complement(28625..29323) [in contig]
Type	CDS
Length	699 bp (232 aa)

Annotation

Category	3.2 modification methylation
Product	putative methyltransferase
Product (GenBank)	methyl transferase
Gene
Gene (GenBank)	pyr22
EC number	2.1.1.-
Keyword
Note
Note (GenBank)
Reference	ACC A3R4R3 PmId [17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007) comment pyrrolomycin biosynthetic gene clustersに関する文献。 pyr22(232 a.a.): methyl transferase pyr22自体の機能実験は行われていないが、相同性よりmethyltransferaseの機能を持つと予測している。 aryl O-methyl groupの導入を担う可能性が記されている。
Family/Domain	FD IPR013216 comment [IPR013216] Methyltransferase type 11 (Domain) Function GO:0008168 methyltransferase activity

Sequence

>putative methyltransferase [methyl transferase]
MNETNAFFQAVYRHDSEFGNQSPPWDIDGPQPVFVDLANSGKISGEVLDAGFGPAGLSLY
LATEGYAVTGIDQSPEAVDAARARARQRGLRASFAVGDLLDLSEYRGRFGTVVECGVFHG
LGPDERRQYARSLHVATRPGAHAHLLNLTGSGQEAARARLAESGMSAPAVEGFHAVTAEH
LRAAFAGLWDVVSIEERAMKLRFPGDQQFTELPAVLSTFKRRPDVRCTGAAK

>putative methyltransferase [methyl transferase]
ATGAACGAGACCAACGCCTTCTTCCAGGCTGTCTACCGTCATGACTCCGAATTCGGAAAC
CAGAGTCCGCCCTGGGACATCGACGGTCCGCAGCCGGTCTTCGTCGATCTCGCCAATTCG
GGAAAGATCAGCGGTGAGGTGCTCGACGCCGGTTTCGGACCCGCGGGACTGAGCCTGTAC
CTGGCCACCGAGGGCTACGCGGTCACCGGGATCGACCAGTCGCCCGAGGCGGTTGACGCG
GCCCGGGCGCGGGCACGCCAGCGCGGCCTGCGCGCGTCGTTCGCCGTTGGGGACCTGCTG
GACCTCTCGGAGTACCGCGGGCGCTTCGGCACGGTCGTGGAGTGTGGCGTCTTCCACGGC
CTCGGCCCCGACGAGCGCCGGCAGTACGCCCGATCACTGCACGTGGCGACCAGGCCAGGG
GCCCACGCCCACCTGCTCAACCTGACCGGCAGCGGCCAGGAGGCCGCCCGGGCCCGCCTC
GCCGAGTCGGGGATGTCCGCGCCGGCGGTGGAGGGGTTCCACGCCGTGACCGCGGAACAC
CTGCGAGCCGCTTTCGCCGGACTCTGGGACGTGGTGTCGATCGAGGAACGAGCCATGAAA
CTCAGGTTCCCGGGCGACCAGCAATTCACCGAACTTCCCGCCGTGTTGTCCACGTTCAAA
AGGCGGCCCGACGTTCGGTGTACGGGGGCCGCGAAATAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR013216 Methyltransferase type 11 (Domain) PF08241 Methyltransf_11
SignalP	No significant hit
TMHMM	No significant hit

Pyrlo_00210 Pyrlo_00230