Pyrlo_00200 Pyrlo_00200   Pyrlo_00220 Pyrlo_00220

Pyrlo_00210 : CDS information

close this sectionLocation

Organism
StrainATCC 31673 (=NBRC 14294)
Entry namePyrrolomycin
Contig
Start / Stop / Direction27,983 / 28,297 / + [in whole cluster]
27,983 / 28,297 / + [in contig]
Location27983..28297 [in whole cluster]
27983..28297 [in contig]
TypeCDS
Length315 bp (104 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative monooxygenase
Product (GenBank)monooxygenase
Gene
Gene (GenBank)pyr21
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007)
comment
pyrrolomycin biosynthetic gene clustersに関する文献。

pyr21(104 a.a.): monooxygenase

pyr21自体の機能実験は行われていないが、相同性よりmonooxygenaseと予測している。
本ORFもしくはpyr2のどちらかが、pyrrolomycin骨格にnitro基を導入する機能を持つ可能性を記載している。
Related Reference
ACC
Q9L4Y0
NITE
Ello_00080
PmId
[11325225] Cloning, sequencing, and heterologous expression of the elmGHIJ genes involved in the biosynthesis of the polyketide antibiotic elloramycin from Streptomyces olivaceus Tu2353. (J Nat Prod. , 2001)
comment
<Tcm F1 monooxygenase(elmH), Streptomyces olivaceus, BLAST 19; id=41%>
[Ello_00080]tetracenomycin F1 C-5 monooxygenase

E. coliでelmHを過剰発現、ElmH精製、活性確認。
TcmF1→TcmD3へのoxidationをTLCで基質変換をみることにより確認。

close this sectionSequence

selected fasta
>putative monooxygenase [monooxygenase]
MTANPTDVITLVNVYSVEPERQDELVDLLYRTADELMRHQPGFITAHIYRSMDGTRVTNY
AQWRSMADFQAVQQLPEAAKYVKASAELGASDPHVYTVASVHTA
selected fasta
>putative monooxygenase [monooxygenase]
ATGACAGCGAACCCCACCGATGTCATCACCCTGGTCAACGTCTACTCGGTCGAGCCGGAG
CGTCAGGACGAGCTGGTCGACCTGCTGTACCGCACCGCTGACGAACTGATGCGGCACCAG
CCCGGCTTCATCACCGCCCACATCTACCGCAGCATGGACGGCACCCGGGTGACCAACTAC
GCGCAGTGGCGGTCAATGGCCGACTTCCAGGCCGTGCAGCAGCTCCCCGAGGCGGCGAAG
TACGTGAAGGCGTCCGCCGAGCTCGGTGCGTCCGACCCCCACGTCTACACCGTGGCGTCG
GTCCACACCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR007138 Antibiotic biosynthesis monooxygenase (Domain)
[9-83] 5.69999999999998e-19 PF03992
PF03992   ABM
IPR011008 Dimeric alpha-beta barrel (Domain)
[8-102] 7.30000590915208e-24 SSF54909
SSF54909   Dimer_A_B_barrel
SignalP No significant hit
TMHMM No significant hit
Pyrlo_00200 Pyrlo_00200   Pyrlo_00220 Pyrlo_00220
Page top