Pyrlo_00320 : CDS information

Location

Organism	Actinosporangium vitaminophilum
Strain	ATCC 31673 (=NBRC 14294)
Entry name	Pyrrolomycin
Contig	EF140901
Start / Stop / Direction	51,057 / 52,499 / + [in whole cluster] 51,057 / 52,499 / + [in contig]
Location	51057..52499 [in whole cluster] 51057..52499 [in contig]
Type	CDS
Length	1,443 bp (480 aa)

Annotation

Category	5.1 general function
Product	putative homogentisate 1,2-dioxygenase
Product (GenBank)	homogentisate 1,2-dioxygenase
Gene
Gene (GenBank)
EC number	1.13.11.5
Keyword
Note	It is supposed that this ORF is not involved in pyrrolomycin biosynthesis.
Note (GenBank)	orf32
Reference	ACC A3R4S3 PmId [17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007) comment pyrrolomycin biosynthetic gene clustersに関する文献。 orf32(480 a.a.): homogentisate 1,2-dioxygenase orf32はpyrrolomycin biosynthesisとは関係無いであろうとの記載有り。相同性よりhomogentisate 1,2-dioxygenaseと予測。
Related Reference	ACC Q9X4F5 PmId [10220173] Identification of a novel nutrient-deprivation-induced Sinorhizobium meliloti gene (hmgA) involved in the degradation of tyrosine. (Microbiology. , 1999) comment <Homogentisate 1,2-dioxygenase(hmgA), Rhizobium meliloti, BLAST list 154; id=57%> hmgA挿入変異株についての解析を行い、その表現型がA. nidulansのhmgA変異株の表現型と似ていることより、真核生物におけるhmgAと同等の機能を持つとしている。

Sequence

>putative homogentisate 1,2-dioxygenase [homogentisate 1,2-dioxygenase]
MVEHRNQPSPFPSSGTGAHPMSSPSTAAEQAGTPAAGLDYSPGFGNEHVSEAVPGALPVG
RNSPQRAPLGLYAEQLSGTAFTEPRHANRRSWLYRIRPSAAHPRFRRTDNGALLSAPFTD
SEPDPNRLRWDPLPLPERDTDFVTGLYTVGGNGDVLRRCGIGIHLYRANRSMVDRVFVDS
DGELLIVPELGGLLVRTELGLLEARPGQVALVPRGVRFRVELLDGAARGYVCENYGQPFR
LPDLGPIGANGLANPRDFLAPVAAFEDVEEPVEVVNKFGGNLWAAQYDHSPLDVVAWHGN
YAPYVYDLSRFNVLGSISFDHPDPSIFTVLTSPSSTPGLANADFVVFAPRWLVGEDTFRP
AYFHRNVMSEFMGLIDGAYDAKASGFAPGGASLHNMMSAHGPDRDTYAKASSAELLPEKV
DGGTAFMFETRWPVVVTAQAMAADHRQQDYDAVWRDIGRGFPGTAPADGPVRSAGPERTR

>putative homogentisate 1,2-dioxygenase [homogentisate 1,2-dioxygenase]
ATGGTCGAGCATAGGAATCAACCGAGCCCCTTCCCCTCGTCCGGCACAGGAGCGCACCCC
ATGAGCAGCCCCAGCACAGCAGCAGAGCAGGCCGGCACGCCCGCCGCGGGCCTCGACTAC
TCGCCGGGCTTCGGGAACGAGCACGTCAGTGAGGCAGTGCCGGGAGCGTTGCCGGTCGGG
CGGAACTCCCCGCAGCGCGCACCGCTCGGCCTGTACGCCGAGCAGTTGAGCGGTACGGCC
TTCACCGAGCCCCGGCACGCCAACCGCAGGAGCTGGCTGTACCGCATCCGCCCCTCCGCC
GCGCACCCGAGGTTCCGCAGGACCGACAACGGTGCCCTGCTCAGCGCGCCGTTCACCGAC
AGCGAGCCCGACCCGAACCGGCTGCGCTGGGACCCGCTGCCGCTGCCCGAGCGGGACACC
GACTTCGTCACCGGGCTGTACACCGTCGGGGGCAACGGCGACGTGCTGCGCCGCTGCGGC
ATCGGCATCCACCTGTACCGCGCCAACCGCTCCATGGTGGACCGGGTGTTCGTGGACTCC
GACGGGGAGCTGCTGATCGTCCCGGAGCTGGGCGGGCTGCTGGTGCGCACCGAGCTGGGG
CTGCTCGAGGCACGGCCCGGGCAGGTGGCACTGGTGCCCCGCGGGGTGCGGTTCCGGGTG
GAGCTGCTCGACGGGGCGGCGCGGGGCTACGTCTGTGAGAACTACGGTCAGCCGTTCCGG
CTCCCCGACCTGGGGCCGATCGGTGCGAACGGCCTGGCCAACCCCCGGGACTTCCTGGCG
CCCGTGGCGGCCTTCGAGGACGTCGAGGAGCCGGTGGAGGTGGTGAACAAGTTCGGTGGG
AACCTCTGGGCCGCCCAGTACGACCACTCACCGCTCGACGTGGTCGCCTGGCACGGCAAC
TACGCCCCCTACGTCTACGACCTGTCCAGGTTCAACGTCCTCGGCTCGATCAGCTTCGAC
CACCCGGACCCGTCGATCTTCACCGTGCTGACCTCGCCGTCCAGCACGCCCGGCCTGGCC
AACGCCGACTTCGTGGTCTTCGCGCCCCGTTGGCTGGTCGGCGAGGACACCTTCCGGCCG
GCGTACTTCCACCGCAACGTGATGAGCGAGTTCATGGGTCTGATCGACGGCGCCTACGAC
GCCAAGGCCAGCGGGTTCGCGCCGGGCGGGGCCAGCCTGCACAACATGATGAGCGCGCAC
GGGCCCGACCGGGACACCTACGCCAAGGCCAGCAGCGCCGAGCTGCTGCCGGAGAAGGTG
GACGGGGGCACGGCCTTCATGTTCGAGACCCGCTGGCCGGTGGTGGTCACCGCGCAGGCG
ATGGCGGCGGACCACCGGCAGCAGGACTACGACGCCGTCTGGCGGGACATCGGTCGGGGC
TTCCCCGGGACGGCGCCGGCCGACGGGCCCGTCCGGTCGGCGGGGCCGGAGCGGACCAGG
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005708 Homogentisate 1,2-dioxygenase (Family) TIGR01015 HmgA PF04209 HgmA IPR011051 RmlC-like cupin domain (Domain) SSF51182 RmlC_like_cupin
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Pyrlo_00310 Pyrlo_00330