Pyrlo_00350 : CDS information

Location

Organism	Actinosporangium vitaminophilum
Strain	ATCC 31673 (=NBRC 14294)
Entry name	Pyrrolomycin
Contig	EF140901
Start / Stop / Direction	54,492 / 55,655 / + [in whole cluster] 54,492 / 55,655 / + [in contig]
Location	54492..55655 [in whole cluster] 54492..55655 [in contig]
Type	CDS
Length	1,164 bp (387 aa)

Annotation

Category	5.1 general function
Product	putative alpha-methylacyl-CoA racemase
Product (GenBank)	L-carnitine dehydratase
Gene
Gene (GenBank)
EC number	5.1.99.4
Keyword
Note	It is supposed that this ORF is not involved in pyrrolomycin biosynthesis.
Note (GenBank)	orf35
Reference	ACC A3R4S6 PmId [17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007) comment pyrrolomycin biosynthetic gene clustersに関する文献。 orf35(387 a.a.): L-carnitine dehydratase orf35 はpyrrolomycin biosynthesisとは関係無いであろうとの記載があるorf32よりも外側に位置しており、orf32同様にpyrrolomycin biosynthesisとは関係無いと予想されている模様。相同性より L-carnitine dehydrataseと予測。
Related Reference	ACC Q5W8V4 PmId [15489432] Role of alpha-methylacyl coenzyme A racemase in the degradation of methyl-branched alkanes by Mycobacterium sp. strain P101. (J Bacteriol. , 2004) comment <Alpha-Methylacyl-CoA racemase(mcr), Mycobacterium sp. P101, BLAST list 66; id=49%> mcr (alpha-methylacyl coenzyme A [alpha-methylacyl-CoA] racemase gene)をE. coliで発現させて、(R)-2-methylpentadecanoyl-CoAのracemizationを行うことを確認している。

Sequence

>putative alpha-methylacyl-CoA racemase [L-carnitine dehydratase]
MGTDTEGAPGRGGPLAGVRVLELGGIGPGPFCGMVLADMGADVIRVERPGDRGALAMPVL
LRGRRSVALDLKDPVAARAVRSIAERCDALIEGFRPGVTERLGLGPEELCQANPRLVYAR
MTGWGQDGPHAQAPGHDINYLALSGALHTFGPADGDPVPPLNLVADFGGGGMLLALGVVS
ALLRAQRDGRGQVVDASMVDGTALLLAMTYGFLANGLWTDQRGVNLLDGSGPFYRVYRCR
DQRHVAVGCLEEQFRADLLRVLGLTDDPDFATPDDRKNWPVMNQRLEQIFTSRTRDNWAA
LFEGTQACVTPVLSLNEAAAHPHNLARGTYLAGPTGIVQPSPAPRFGGTPSDAPRAAPTP
GEHTAEVLAEAGLTSAEIAALTPEPGR

>putative alpha-methylacyl-CoA racemase [L-carnitine dehydratase]
ATGGGCACCGACACCGAAGGGGCCCCGGGCCGGGGGGGCCCGCTCGCCGGGGTGCGGGTG
CTGGAACTCGGCGGCATCGGCCCGGGACCGTTCTGCGGCATGGTCCTCGCCGACATGGGC
GCCGACGTCATCAGGGTGGAGCGCCCCGGGGACCGCGGCGCGCTGGCCATGCCGGTGCTG
CTGCGGGGACGCCGTTCCGTCGCCCTGGACCTGAAGGACCCCGTCGCGGCACGAGCGGTC
CGCAGCATCGCCGAGCGCTGCGACGCGCTGATCGAGGGGTTCCGGCCGGGGGTGACCGAG
CGGCTCGGGCTCGGGCCGGAGGAGCTGTGCCAGGCCAACCCCCGCCTGGTGTACGCCCGG
ATGACCGGCTGGGGGCAGGACGGCCCGCACGCCCAGGCGCCCGGTCACGACATCAACTAC
CTGGCGCTCTCGGGGGCGCTGCACACCTTCGGCCCCGCGGACGGTGACCCCGTTCCCCCG
TTGAACCTGGTCGCCGACTTCGGCGGCGGAGGCATGCTGCTCGCCCTCGGCGTGGTCAGC
GCCCTGCTCCGGGCCCAGCGTGACGGCCGGGGCCAGGTGGTGGACGCCTCCATGGTGGAC
GGGACCGCCCTGCTGCTCGCCATGACCTACGGCTTCCTCGCCAACGGGCTGTGGACCGAC
CAGCGCGGGGTCAACCTGCTCGACGGCAGCGGCCCCTTCTACCGGGTCTACCGGTGCCGT
GACCAGCGCCACGTGGCGGTGGGCTGCCTCGAGGAGCAGTTCCGCGCCGACCTGCTGCGG
GTCCTCGGCCTCACCGACGACCCGGACTTCGCGACCCCGGACGACCGGAAGAACTGGCCG
GTGATGAACCAGCGCCTGGAGCAGATCTTCACCAGCCGCACCCGCGACAACTGGGCCGCC
CTGTTCGAGGGCACCCAGGCGTGCGTCACCCCGGTGCTGTCCCTGAACGAGGCCGCGGCG
CACCCGCACAACCTCGCGCGCGGCACCTACCTCGCCGGCCCCACCGGGATCGTCCAACCC
AGCCCCGCGCCGCGGTTCGGGGGGACGCCGAGCGACGCGCCGAGGGCCGCGCCCACACCC
GGCGAACACACCGCGGAGGTGCTGGCCGAAGCGGGGCTCACCAGTGCGGAGATCGCTGCC
CTGACCCCGGAGCCGGGCCGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003673 CoA-transferase family III (Family) PF02515 CoA_transf_3 IPR023606 CoA-transferase family III domain (Domain) SSF89796 CoA-Trfase_fam_III G3DSA:3.40.50.10540 CoA-Trfase_fam_III
SignalP	No significant hit
TMHMM	No significant hit

Pyrlo_00340 last last