Pyrlo_00340 : CDS information

Location

Organism	Actinosporangium vitaminophilum
Strain	ATCC 31673 (=NBRC 14294)
Entry name	Pyrrolomycin
Contig	EF140901
Start / Stop / Direction	53,341 / 54,492 / + [in whole cluster] 53,341 / 54,492 / + [in contig]
Location	53341..54492 [in whole cluster] 53341..54492 [in contig]
Type	CDS
Length	1,152 bp (383 aa)

Annotation

Category	5.1 general function
Product	putative acyl-CoA dehydrogenase
Product (GenBank)	acyl-CoA dehydrogenase
Gene
Gene (GenBank)
EC number	1.3.99.-
Keyword
Note	It is supposed that this ORF is not involved in pyrrolomycin biosynthesis.
Note (GenBank)	orf34
Reference	ACC A3R4S5 PmId [17158935] Cloning and characterization of the pyrrolomycin biosynthetic gene clusters from Actinosporangium vitaminophilum ATCC 31673 and Streptomyces sp. strain UC 11065. (Antimicrob Agents Chemother. , 2007) comment pyrrolomycin biosynthetic gene clustersに関する文献。 orf34(383 a.a.): acyl CoA dehydrogenase orf34はpyrrolomycin biosynthesisとは関係無いであろうとの記載があるorf32よりも外側に位置しており、orf32同様にpyrrolomycin biosynthesisとは関係無いと予想されている模様。相同性より acyl CoA dehydrogenaseと予測。
Related Reference	ACC P28330 PmId [8155643] Identification of the catalytic base in long chain acyl-CoA dehydrogenase. (Biochemistry. , 1994) comment Blast 432nd, id41%, 4e-85 Human_ACADL Long-chain specific acyl-CoA dehydrogenase, mitochondrial(EC 1.3.99.13) mutationを入れた配列でクローニングし、酵素精製、活性を見ている。

Sequence

>putative acyl-CoA dehydrogenase [acyl-CoA dehydrogenase]
MRRTVFNDDHEAFRETVRAFLTKEVVPQYAQWEQLGHPPREFYRRMGALGLLGIAVPEEF
GGGGASGLRFSAVLSEETARHGVTLGASRVHSDLVLPYLLEYATPEQKQRWLPGFASGDL
MTAIAMTEPGTGSDLAGITTTARRDGDVYVLNGSKTFITGGALADLVLVVCRTAPRQDGD
RRAGLSILVVEATSPGFSVGRKLEKIGLRTQDTAELFFDEVRVPAANLLGEEGEAFGYLS
HNLVQERLAIVIGAVTAAAAAVEMTVEYVRQREVFGKPLSSFQNTKFVLAECAAEVEAGQ
VLMDRAIEEHERGELSHADVAKAKLFCTEMEGRVIDKCLQLHGGYGYMLEYPIARLYADA
RVHRVYGGTSEVQKSIVGKSLGL

>putative acyl-CoA dehydrogenase [acyl-CoA dehydrogenase]
GTGCGCCGCACCGTGTTCAACGATGACCACGAGGCATTCCGGGAGACCGTCCGGGCGTTC
CTCACCAAGGAGGTCGTCCCGCAGTACGCGCAGTGGGAGCAGCTCGGCCACCCTCCCCGT
GAGTTCTACCGACGGATGGGCGCGCTGGGGCTCCTGGGCATCGCGGTCCCCGAGGAGTTC
GGCGGTGGCGGGGCCAGCGGGCTGAGGTTCAGCGCCGTGCTCTCCGAGGAGACCGCCCGG
CACGGAGTCACCCTGGGCGCCAGCCGGGTCCACTCCGACCTGGTCCTGCCGTACCTGTTG
GAGTACGCCACCCCCGAGCAGAAGCAGCGGTGGCTGCCGGGCTTCGCCAGCGGCGACCTG
ATGACCGCGATCGCCATGACGGAGCCGGGGACCGGGTCCGACCTGGCCGGGATCACCACC
ACCGCCCGCCGGGACGGCGACGTCTACGTGCTCAACGGCTCCAAGACGTTCATCACCGGC
GGGGCCCTCGCCGACCTGGTGCTGGTGGTCTGCCGCACCGCCCCGCGGCAGGACGGCGAC
CGTCGGGCCGGGCTGAGCATCCTGGTGGTGGAGGCCACGTCCCCGGGTTTCAGCGTCGGC
CGGAAGCTGGAGAAGATCGGCTTGCGCACCCAGGACACCGCGGAGCTGTTCTTCGACGAG
GTGCGGGTGCCCGCGGCCAACCTCCTGGGCGAGGAGGGCGAGGCGTTCGGCTACCTCTCC
CACAACCTGGTGCAGGAACGACTCGCCATCGTCATCGGAGCGGTGACCGCCGCCGCGGCG
GCGGTGGAGATGACCGTGGAGTACGTCCGCCAGCGCGAGGTGTTCGGCAAGCCGCTGTCC
TCGTTCCAGAACACCAAGTTCGTGCTCGCCGAGTGCGCCGCCGAGGTGGAGGCCGGTCAG
GTGCTGATGGACCGGGCGATCGAGGAGCACGAGCGCGGGGAGCTGAGCCACGCGGACGTG
GCCAAGGCCAAGCTGTTCTGCACCGAGATGGAGGGCCGCGTGATCGACAAGTGCCTCCAG
CTCCACGGGGGGTACGGCTACATGCTCGAGTACCCGATCGCCCGGCTGTACGCCGACGCC
CGGGTGCACCGGGTGTACGGCGGGACCAGCGAGGTGCAGAAGTCCATCGTCGGCAAGTCG
CTGGGCCTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006089 Acyl-CoA dehydrogenase, conserved site (Conserved_site) PS00073 ACYL_COA_DH_2 IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain) PF00441 Acyl-CoA_dh_1 IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain) PF02770 Acyl-CoA_dh_M G3DSA:2.40.110.10 Acyl_CoA_DH/ox_M IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain) PF02771 Acyl-CoA_dh_N IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain) SSF47203 AcylCoADH_C_like G3DSA:1.20.140.10 AcylCoA_DH_1/2_C IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain) SSF56645 AcylCoA_dehyd_NM IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain) G3DSA:1.10.540.10 AcylCoA_DH/ox_N
SignalP	No significant hit
TMHMM	No significant hit

Pyrlo_00330 Pyrlo_00350