R1128_00040 R1128_00040   R1128_00060 R1128_00060

R1128_00050 : CDS information

close this sectionLocation

Organism
StrainR1128
Entry nameR1128
Contig
Start / Stop / Direction6,651 / 6,214 / - [in whole cluster]
6,651 / 6,214 / - [in contig]
Locationcomplement(6214..6651) [in whole cluster]
complement(6214..6651) [in contig]
TypeCDS
Length438 bp (145 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
Product (GenBank)biotin carboxylase carrier protein
Gene
Gene (GenBank)zhuE
EC number6.4.1.2
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[10931852] Cloning, nucleotide sequence, and heterologous expression of the biosynthetic gene cluster for R1128, a non-steroidal estrogen receptor antagonist. Insights into an unusual priming mechanism. (J Biol Chem. , 2000)
comment
R1128 gene clusterがあると予測されるStreptomyces sp. R1128の40kb領域をS.lividans K4-114で異種性発現し、産物としてR1128Cと新規産物HU235を検出。
この領域をシークエンスして、生合成genesがすべて含まれていそうな17kb領域をサブクローニング、oligonucleotide walkingして正確な配列を得た。

zhuE: Biotin carboxylate carrier protein (BCCP)

機能提唱は配列解析に基づく。
ZhuD, ZhuE, ZhuFはacetyl-CoA carboxylase subunitsに相同性あり。
jadomycin clusterにあるZhuD, ZhuE homologの不活化によりjadomycin生合成が著しく減ることから、polyketide生合成のためのmalonyl-CoA合成に関与するとされている。
Related Reference
ACC
P0ABD8
PmId
[8631788] Purification and characterization of intact and truncated forms of the Escherichia coli biotin carboxyl carrier subunit of acetyl-CoA carboxylase. (J Biol Chem. , 1996)
[9398236] Structure of the carboxy-terminal fragment of the apo-biotin carboxyl carrier subunit of Escherichia coli acetyl-CoA carboxylase. (Biochemistry. , 1997)
comment
Blast 230th, id40%, 1e-13
E.coli_accB
Biotin carboxyl carrier protein of acetyl-CoA carboxylase

--
[PMID: 8631788](1996)
acetyl-CoA carboxylaseのBCCP (Biotin carboxyl carrier protein)について、
intact BCCP (156aa)、C末truncated BCCP (87aa)両方を精製し、活性を比較。
biotin-accepting activityは同程度で、機能的な違いはない、との結果。

--
[PMID: 9398236](1997)
構造解析

close this sectionSequence

selected fasta
>putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit [biotin carboxylase carrier protein]
MLEALPERPTRLNVRADGVGIEVSWEAPQAPAVPQPVVSAVPVATVPAVAAPPPVPAPPA
EPTAHQVCAPSVGVFYHATEPGAAPFVRVGDVVRPGQQIGIVEAMKLMLPVEADRPGRVS
AILRANGDQVEYGEPLVALVPVEGA
selected fasta
>putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit [biotin carboxylase carrier protein]
ATGCTCGAAGCGTTGCCCGAGCGGCCCACGCGGCTCAACGTGCGCGCGGACGGCGTCGGG
ATCGAGGTGTCCTGGGAGGCGCCGCAGGCCCCGGCGGTGCCCCAGCCCGTCGTGTCCGCC
GTTCCCGTCGCCACCGTGCCCGCCGTCGCCGCGCCGCCGCCCGTGCCCGCGCCCCCGGCG
GAGCCGACCGCGCACCAGGTGTGCGCGCCCAGCGTCGGCGTGTTCTACCACGCCACCGAG
CCCGGCGCCGCGCCGTTCGTGCGGGTCGGCGACGTGGTCCGCCCCGGTCAACAGATCGGC
ATCGTCGAGGCGATGAAGCTCATGCTCCCCGTCGAGGCCGACCGTCCCGGCCGGGTGTCC
GCGATCCTGCGCGCCAACGGCGACCAGGTCGAGTACGGCGAGCCGCTCGTCGCGCTGGTC
CCCGTGGAAGGCGCGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000089 Biotin/lipoyl attachment (Domain)
[65-139] PS50968
PS50968   BIOTINYL_LIPOYL
[65-138] 1.3e-18 PF00364
PF00364   Biotin_lipoyl
IPR001249 Acetyl-CoA biotin carboxyl carrier (Family)
[69-82] 4.60000044330862e-12 PR01071 [86-100] 4.60000044330862e-12 PR01071 [101-114] 4.60000044330862e-12 PR01071
PR01071   ACOABIOTINCC
IPR001882 Biotin-binding site (Binding_site)
[96-113] PS00188
PS00188   BIOTIN
IPR011053 Single hybrid motif (Domain)
[61-139] 6.19998763505952e-20 SSF51230
SSF51230   Hybrid_motif
SignalP
[1-16] 0.076 Signal
Eukaryota   
TMHMM No significant hit
R1128_00040 R1128_00040   R1128_00060 R1128_00060
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