R1128_00060 : CDS information

Location

Organism	Streptomyces sp.
Strain	R1128
Entry name	R1128
Contig	AF293442
Start / Stop / Direction	8,901 / 7,165 / - [in whole cluster] 8,901 / 7,165 / - [in contig]
Location	complement(7165..8901) [in whole cluster] complement(7165..8901) [in contig]
Type	CDS
Length	1,737 bp (578 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative acetyl-CoA carboxylase carboxyltransferase subunit
Product (GenBank)	acetyl carboxylase
Gene
Gene (GenBank)	zhuF
EC number	6.4.1.2
Keyword
Note
Note (GenBank)
Reference	ACC Q9F6D6 PmId [10931852] Cloning, nucleotide sequence, and heterologous expression of the biosynthetic gene cluster for R1128, a non-steroidal estrogen receptor antagonist. Insights into an unusual priming mechanism. (J Biol Chem. , 2000) comment R1128 gene clusterがあると予測されるStreptomyces sp. R1128の40kb領域をS.lividans K4-114で異種性発現し、産物としてR1128Cと新規産物HU235を検出。この領域をシークエンスして、生合成genesがすべて含まれていそうな17kb領域をサブクローニング、oligonucleotide walkingして正確な配列を得た。 zhuF: Acetyl carboxylase subunit alpha(AC alpha) + subunit beta(AC beta) 機能提唱は配列解析に基づく。 ZhuD, ZhuE, ZhuFはacetyl-CoA carboxylase subunitsに相同性あり。
Related Reference	ACC P0A9Q5 PmId [1355086] The dedB (usg) open reading frame of Escherichia coli encodes a subunit of acetyl-coenzyme A carboxylase. (J Bacteriol. , 1992) [1355089] The genes encoding the two carboxyltransferase subunits of Escherichia coli acetyl-CoA carboxylase. (J Biol Chem. , 1992) [16460018] The structure of the carboxyltransferase component of acetyl-coA carboxylase reveals a zinc-binding motif unique to the bacterial enzyme. (Biochemistry. , 2006) [19965770] A tale of two functions: enzymatic activity and translational repression by carboxyltransferase. (Nucleic Acids Res. , 2010) comment >for N-terminal Blast list外、id50.6% N末hit E.coli_accD(dedB, usg) Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta(EC 6.4.1.2) -- [PMID: 1355086](1992) E.coli_dedB (usg)の同定。 -- [PMID:1355089](1992) 精製されたE.coli acetyl-CoA carboxylase componentのpeptide mapping 35-kDa alpha subunit 33-kDa beta subunit それぞれのgene cloning alpha: accA beta: accD (<-dedB/usg) -- [PMID:16460018](2006) S. aureus と E. coli由来carboxyltransferase subunitsの構造解析 -- [PMID:19965770](2010) acetyl-CoA carboxylaseのcarboxyltransferase subunitは、それ自身をコードするaccA and accDのmRNAsを結合することで翻訳が抑制される。これはacetyl-CoAによって解放される。この相互作用はbeta-subunit(AccD)のzinc fingerによって媒介される。
Related Reference	ACC P0ABD5 PmId [1355089] The genes encoding the two carboxyltransferase subunits of Escherichia coli acetyl-CoA carboxylase. (J Biol Chem. , 1992) [15066985] Identification and characterization of the first class of potent bacterial acetyl-CoA carboxylase inhibitors with antibacterial activity. (J Biol Chem. , 2004) [16460018] The structure of the carboxyltransferase component of acetyl-coA carboxylase reveals a zinc-binding motif unique to the bacterial enzyme. (Biochemistry. , 2006) [19965770] A tale of two functions: enzymatic activity and translational repression by carboxyltransferase. (Nucleic Acids Res. , 2010) comment >for C-terminal Blast list外、id51.79% C末hit E.coli_accA Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha(EC 6.4.1.2)

Sequence

>putative acetyl-CoA carboxylase carboxyltransferase subunit [acetyl carboxylase]
MTETAIRPTTGVADWVQCGNCRAAVYGKRWARGLRVCPECAKHHPVGARERLDQLLDPGS
AEPIRNDVRTFDVLGFTDTKPYPARLAAARAATGLDEAVVIVTGRIHGQPLVVAAMDFAF
LGGSLGGAVGELITLAAETALAEHLPLLIVSASGGARMQEGAVALMQMAKTSAALGQLDE
AGVLTISLVTDPTYGGVAASFATLCDVIVAEPGARLGFAGRRVIAQTIREELPPEFQTAE
YLLEHGLIDLIVPRRALRDELARLLRAGAKRPKGGDGEPARRGGLDAGASPYVVDPADLA
AHDPSRQVRAARAIDRPTTLDYIALLCEDFVELRGDRISGDCPAIVAGLARFGGRPVAVI
GHQKGHDAQELRRRNFGMPIPSGYRKAARIMRLADKLGLPVVTFVDTPGAYPGAVAEEQG
QAVAIAENLRLMAGLRVPVVTVVTGEGGSGGALALAVANRVLMFENGIYSVISPEGCAAI
LWQDPAAAGRAARALRVTARELLGLGIVDGVLPEPEGGTGADPLRAADALRDAIAAGLAE
LSPLDGHQLVADRWARFRRYGAERTTDAEITAMKGDPT

>putative acetyl-CoA carboxylase carboxyltransferase subunit [acetyl carboxylase]
ATGACCGAAACCGCCATCCGGCCCACGACCGGCGTCGCCGACTGGGTGCAGTGCGGCAAC
TGCCGCGCCGCCGTGTACGGCAAGCGCTGGGCCCGCGGCCTGCGGGTCTGCCCCGAATGC
GCCAAACACCACCCGGTCGGCGCCCGCGAACGCCTCGACCAGCTCCTCGACCCCGGCTCC
GCCGAGCCGATCCGCAACGACGTGCGCACCTTCGACGTGCTCGGCTTCACCGACACCAAG
CCCTACCCCGCGCGGCTCGCCGCCGCGCGTGCCGCCACCGGGCTCGACGAGGCCGTCGTC
ATCGTGACCGGCCGGATCCACGGCCAGCCGCTCGTCGTCGCCGCGATGGACTTCGCGTTC
CTCGGCGGCAGCCTGGGCGGCGCGGTCGGCGAACTGATCACCCTCGCCGCCGAGACCGCG
CTCGCCGAACACCTGCCGCTGCTGATCGTCAGCGCGTCCGGCGGCGCGCGCATGCAGGAA
GGCGCCGTCGCGCTCATGCAGATGGCCAAGACCAGCGCCGCCCTCGGGCAACTGGACGAG
GCCGGCGTGTTGACCATCTCGCTGGTCACCGACCCCACCTACGGCGGCGTCGCGGCGTCC
TTCGCGACCCTGTGCGACGTCATCGTCGCCGAACCCGGCGCGCGTCTCGGCTTCGCCGGC
CGGCGGGTCATCGCGCAGACCATCCGCGAGGAACTGCCGCCGGAGTTCCAGACCGCCGAA
TACCTCCTCGAACACGGCCTGATCGACCTGATCGTGCCGCGCCGCGCGCTGCGCGACGAA
CTGGCCCGCCTGCTGCGGGCCGGCGCGAAGCGGCCCAAGGGCGGGGACGGGGAACCGGCC
CGACGCGGCGGCCTGGACGCGGGGGCATCGCCGTACGTCGTCGACCCCGCCGACCTCGCC
GCGCACGACCCCAGCCGCCAGGTGCGCGCCGCGCGGGCCATCGACCGGCCCACCACGCTC
GACTACATCGCGCTGCTGTGCGAGGACTTCGTCGAACTGCGCGGCGACCGCATCTCGGGC
GACTGCCCCGCCATCGTCGCCGGCCTGGCCCGCTTCGGCGGCCGACCCGTCGCCGTCATC
GGCCACCAGAAGGGCCACGACGCGCAGGAGCTGCGCCGCCGCAACTTCGGCATGCCGATC
CCGTCGGGCTACCGCAAGGCCGCCCGGATCATGCGGCTCGCCGACAAACTCGGTCTCCCG
GTCGTCACGTTCGTCGACACCCCCGGCGCTTACCCCGGCGCGGTCGCCGAGGAGCAGGGC
CAGGCCGTCGCCATCGCCGAGAACCTGCGCCTGATGGCGGGCCTGCGCGTGCCGGTCGTC
ACCGTCGTCACCGGCGAGGGCGGCAGCGGCGGGGCGCTCGCGCTCGCCGTCGCCAACCGT
GTCCTGATGTTCGAGAACGGCATCTACTCGGTCATCAGCCCCGAGGGCTGCGCGGCCATC
CTGTGGCAGGACCCCGCCGCCGCCGGGCGTGCCGCCCGCGCGCTGAGGGTCACCGCCCGC
GAACTCCTCGGCCTGGGCATCGTCGACGGCGTCCTCCCGGAACCCGAAGGCGGCACCGGA
GCCGACCCGCTGCGCGCCGCCGACGCCCTCCGCGACGCGATCGCGGCCGGCCTCGCGGAG
TTGTCCCCCCTCGACGGCCACCAGTTGGTCGCCGACCGCTGGGCCCGCTTCCGCCGCTAT
GGCGCGGAACGCACCACCGACGCCGAGATCACCGCCATGAAAGGCGACCCGACGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000022 Carboxyl transferase (Domain) PF01039 Carboxyl_trans IPR000438 Acetyl-CoA carboxylase carboxyl transferase, beta subunit (Family) TIGR00515 AccD IPR001095 Acetyl-CoA carboxylase, alpha subunit (Family) PF03255 ACCA TIGR00513 AccA PR01069 ACCCTRFRASEA IPR011762 Acetyl-coenzyme A carboxyltransferase, N-terminal (Domain) PS50980 COA_CT_NTER IPR011763 Acetyl-coenzyme A carboxyltransferase, C-terminal (Domain) PS50989 COA_CT_CTER
SignalP	Eukaryota
TMHMM	No significant hit

R1128_00050 R1128_00070