Rimoc_00090 Rimoc_00090   Rimoc_00110 Rimoc_00110

Rimoc_00100 : CDS information

close this sectionLocation

Organism
Strain108
Entry nameRimocidin
Contig
Start / Stop / Direction17,843 / 16,497 / - [in whole cluster]
17,843 / 16,497 / - [in contig]
Locationcomplement(16497..17843) [in whole cluster]
complement(16497..17843) [in contig]
TypeCDS
Length1,347 bp (448 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative crotonyl-CoA reductase
Product (GenBank)RimJ
Gene
Gene (GenBank)rimJ
EC number1.3.1.-
Keyword
  • ethylmalonyl-CoA
Note
Note (GenBank)
  • crotonyl-CoA reductase
Reference
ACC
PmId
[15123265] Starter unit choice determines the production of two tetraene macrolides, rimocidin and CE-108, in Streptomyces diastaticus var. 108. (Chem Biol. , 2004)
[26284936] New Rimocidin/CE-108 Derivatives Obtained by a Crotonyl-CoA Carboxylase/Reductase Gene Disruption in Streptomyces diastaticus var. 108: Substrates for the Polyene Carboxamide Synthase PcsA. (PLoS One. , 2015)
comment
[PMID: 15123265](2004)
Streptomyces diastaticus var. 108におけるrimocidin と CE-108の二つのtetraene macrolidesの生産に関する文献。

rimJ; crotonyl-CoA reductase

rimJ自体の機能解析は行われていないが、相同性よりcrotonyl-CoA reductaseと予想している。
rimJ不活化変異株では、rimocidin産物の割合が下がり、一方でCE-108産物の割合が上昇していることが示されている。

---
[PMID: 26284936](2015)
rimJ破壊株で産生される誘導体についての解析。それらのうちのひとつCE-108Dは、PKS伸長ステップ13でethylmalonyl-CoAの代わりにmethylmalonyl-CoAが取り込まれた構造だった。CE-108Dはcluster外にあるpolyene carboxamide synthase PcsAにより、改善された薬理学的特性を持つCE-108Eへと変換される。
Related Reference
ACC
Q53865
PmId
[8521864] Purification of crotonyl-CoA reductase from Streptomyces collinus and cloning, sequencing and expression of the corresponding gene in Escherichia coli. (Eur J Biochem. , 1995)
comment
<Crotonyl CoA reductase(ccr), Streptomyces collinus, BLAST 10, id=86%>

Streptomyces collinusよりcrotonyl-CoA reductaseを精製し、諸性質を調べている。（ホモダイマーであること、crotonyl-CoA, short-chain-length (C4), enoyl-CoA compoundに対して高い基質特異性を示す一方で、shorter-chain-length (C3), enoyl-CoA thioester, acryloyl-CoAに対しては活性を示さない。）
また、crotonyl-CoA reductase遺伝子をクローニングしてE. coliで発現させて精製し、活性を確認している。

close this sectionSequence

selected fasta
>putative crotonyl-CoA reductase [RimJ]
MKEILNAIQAPDTTAADFAALPLPESYRAITVHKDETDMFEGLAGCDKDPRKSLHLDEVA
VPELGPGEALVAVMASSVNYNSVWTSIFEPLPTFGFLERYGRRSELAKRHDLPYHVIGSD
LAGVVLRTGPGVTVWKPGDEVVAHCLSVELESADGHGDTMLDPEQRIWGFETNFGGLAEL
ALVKSNQLMPKPGHLTWEEAAASGLVNSTAYRQLVSRNGAGMKVGDNVLIWGASGGLGSY
ATQLVLAGGGTPVCVVSNDQKAEICRSMGAEAVIDRNAEGYRFWKDEQTQDPREWKRFGK
RIRELTGGEDVDIVFEHPGRETFGASVFVTRKGGTVVTCASTSGYQHQYDNRYLWMSLKR
IVGSHFANYREAWEANRLIAKGRIHPTLSQTYPLRETGQAAYDVHRNLHQGKVGILALAP
EEGLGVRDAEFRARHASAINRFRGTLED
selected fasta
>putative crotonyl-CoA reductase [RimJ]
GTGAAGGAAATACTGAACGCTATTCAGGCGCCGGACACCACGGCCGCCGACTTCGCCGCG
CTGCCGCTGCCCGAGTCGTACCGCGCGATCACCGTCCACAAGGACGAGACCGACATGTTC
GAGGGGCTCGCGGGGTGCGACAAGGACCCGCGCAAGTCGCTGCACCTCGACGAGGTGGCC
GTCCCCGAACTCGGTCCCGGCGAGGCCCTGGTGGCCGTCATGGCGTCCTCGGTGAACTAC
AACAGCGTCTGGACCTCGATCTTCGAGCCGCTGCCGACGTTCGGGTTCCTGGAGCGGTAC
GGCCGGCGCAGCGAGCTCGCCAAGCGGCACGACCTGCCGTACCACGTCATCGGCTCCGAC
CTGGCGGGCGTCGTGCTGCGCACCGGGCCCGGCGTCACCGTCTGGAAGCCCGGTGACGAG
GTCGTCGCGCACTGCCTGTCCGTCGAGCTGGAGAGCGCGGACGGCCACGGTGACACGATG
CTCGACCCCGAGCAGCGCATCTGGGGCTTCGAGACCAATTTCGGCGGTCTCGCCGAACTC
GCGCTGGTGAAGTCGAATCAGCTGATGCCCAAGCCCGGCCATCTCACGTGGGAGGAGGCG
GCGGCCTCCGGGCTGGTGAATTCCACCGCGTACCGCCAGCTGGTGTCCCGCAACGGCGCG
GGCATGAAGGTCGGTGACAATGTGCTGATCTGGGGCGCGAGCGGCGGACTCGGTTCGTAC
GCGACGCAGTTGGTGCTGGCCGGCGGCGGCACACCCGTCTGTGTGGTGTCCAACGACCAG
AAGGCGGAGATCTGCCGGTCCATGGGCGCGGAAGCGGTCATCGACCGCAACGCCGAGGGG
TACCGGTTCTGGAAGGACGAGCAGACGCAGGACCCCCGGGAGTGGAAGCGGTTCGGCAAG
CGCATCCGCGAGCTGACCGGCGGCGAGGACGTGGACATCGTTTTCGAGCACCCGGGGCGG
GAGACGTTCGGCGCGAGTGTGTTCGTCACGCGCAAGGGCGGCACGGTCGTCACCTGCGCC
TCCACCTCGGGCTACCAGCACCAGTACGACAACCGCTATCTGTGGATGTCGCTCAAGCGC
ATCGTGGGCTCGCACTTCGCCAACTACCGCGAGGCGTGGGAGGCCAACCGCCTGATCGCC
AAGGGCAGGATCCACCCGACGCTGTCCCAGACCTACCCCCTGAGGGAGACCGGGCAGGCG
GCGTACGACGTGCACCGCAACCTCCACCAGGGCAAGGTCGGCATTCTGGCGCTCGCTCCC
GAGGAAGGACTCGGCGTACGGGACGCCGAGTTCCGGGCGCGGCACGCCAGCGCCATCAAC
CGCTTCCGCGGGACGCTGGAGGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR010085 Crotonyl-CoA reductase (Family)
[31-428] TIGR01751
TIGR01751   Crot-CoA-red
IPR011032 GroES-like (Domain)
[39-233] 1.29999924468179e-28 SSF50129
SSF50129   GroES_like
IPR013149 Alcohol dehydrogenase, C-terminal (Domain)
[236-380] 6.90000000000001e-39 PF00107
PF00107   ADH_zinc_N
IPR013154 Alcohol dehydrogenase GroES-like (Domain)
[113-190] 9.7e-07 PF08240
PF08240   ADH_N
IPR016040 NAD(P)-binding domain (Domain)
[232-364] 5.5e-21 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Rimoc_00090 Rimoc_00090   Rimoc_00110 Rimoc_00110
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