Stref_00010 Stref_00010   Stref_00030 Stref_00030

Stref_00020 : CDS information

close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction1,955 / 1,602 / - [in whole cluster]
1,955 / 1,602 / - [in contig]
Locationcomplement(1602..1955) [in whole cluster]
complement(1602..1955) [in contig]
TypeCDS
Length354 bp (117 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in steffimycin biosynthesis.
Note (GenBank)
  • ORF2
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
comment
steffimycin生合成gene clusterのクローニング、特徴づけ。

36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連し、一次代謝関連genesとhousekeeping genesを含む12ORFsによって挟まれている。

orf1-4, 33-36は、S. coelicolor A3(2) and S. avermitilis MA-4680由来の関連タンパクと有意な類似性あり。遺伝子構成も同じ。

orf2はsteffimycin生合成には関係ないとされる12ORFsに含まれるが、異種性steffimycin発現のためのplasmid pEM4STFaには含まれている。

close this sectionSequence

selected fasta
>hypothetical protein [hypothetical protein]
MPDDPAGRIVVVLDLDGVTDKAGLMDRCARDLALPEWFGRNWDALADSLSDPGLWPEGAG
ERGLLVVVVGWRKYAESRPEEWQVAEEIFAEATDRGPGLFVTLGLGGSSEQARDQPG
selected fasta
>hypothetical protein [hypothetical protein]
GTGCCGGACGACCCGGCGGGCCGGATCGTGGTCGTCCTGGACCTCGACGGGGTCACGGAC
AAGGCGGGCCTCATGGACCGCTGCGCCCGTGACCTGGCCCTGCCCGAGTGGTTCGGCCGC
AACTGGGACGCCCTCGCCGACAGCCTGTCCGACCCCGGCCTGTGGCCCGAGGGCGCCGGG
GAGCGGGGGCTGCTGGTCGTCGTCGTGGGCTGGCGGAAGTATGCCGAGTCGCGTCCCGAG
GAGTGGCAGGTCGCCGAGGAGATCTTCGCCGAGGCGACGGACCGGGGCCCGGGCCTCTTC
GTGACCCTCGGCCTTGGAGGATCCTCCGAACAGGCCCGTGACCAGCCTGGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000468 Barstar (barnase inhibitor) (Domain)
[10-99] 3.09999999999998e-21 G3DSA:3.30.370.10
G3DSA:3.30.370.10   G3DSA:3.30.370.10
[11-101] 5.80000000000004e-23 PF01337
PF01337   Barstar
[9-104] 1.39999892049878e-21 SSF52038
SSF52038   SSF52038
SignalP No significant hit
TMHMM No significant hit
Stref_00010 Stref_00010   Stref_00030 Stref_00030
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