Stref_00090 Stref_00090   Stref_00110 Stref_00110

Stref_00100 : CDS information

close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction10,175 / 9,351 / - [in whole cluster]
10,175 / 9,351 / - [in contig]
Locationcomplement(9351..10175) [in whole cluster]
complement(9351..10175) [in contig]
TypeCDS
Length825 bp (274 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative C-7 ketoreductase
Product (GenBank)ketoreductase
Gene
Gene (GenBank)stfT
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
comment
steffimycin生合成gene clusterのクローニング、特徴づけ。
36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連する。

配列解析からの機能推定。
stfT: C-7 ketoreductase

SnoaF,DnrEに似ている。

stfP,K,S(minmal PKS), stfQ(aromatase), stfY(CYC), stfOI(oxygenase), stfX(?), and stfT(C7 KR) genesを含んでいるpEM4KSEHをS.albusに導入し、異種性にsteffimycin中間体(2-O-demethyl-8-demethoxy-10-deoxysteffimycinone)が形成されることを確認している。
Related Reference
ACC
Q53882
NITE
Dauno_00060
PmId
[8655529] Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5. (J Bacteriol. , 1996)
comment
Blast 7th, id59%, 6e-66
Streptomyces sp. (strain C5)_dauE
Aklaviketone reductase

recombinant DauE を含んだStreptomyces lividans TK24 の抽出液は、
aklaviketone → aklavinone
maggiemycin → epsilon-rhodomycinone(RHO)
7-oxodaunomycinone → daunomycinone
へのNADPH-specificな変換を触媒した。
これらの変換はすべてposition 7でのketo基の還元。

close this sectionSequence

selected fasta
>putative C-7 ketoreductase [ketoreductase]
MALAAVPTFGRVTEFEENPKEDAGENADSRGVIVTGGGTGIGRATARAFADRQDRVLVVG
RSASTLTETARGYPNIHTLPVDITEPGAAETIVRAALREFGRIDVLVNNASTAVPAPLEN
IAPAEVDRLLAVNLRAPIALTREALDALTRTRGTVVNLSSAGSLGRRAWPNFSLYGSSKV
ALDFLTRTWAVELAPRGIRVVSVAPGVIETGMGIRMGSSPEEYARFLEWMEKITPLGRVG
QPEEIAWWVVQVAAPEAGFVTGTVLAVDGGASIV
selected fasta
>putative C-7 ketoreductase [ketoreductase]
GTGGCTCTAGCGGCGGTCCCTACCTTCGGCCGCGTGACCGAATTCGAGGAGAACCCGAAG
GAAGACGCCGGGGAGAACGCCGACTCCCGCGGCGTGATCGTCACCGGCGGCGGCACCGGC
ATCGGCCGGGCCACCGCGCGGGCGTTCGCCGACCGCCAGGACAGGGTCCTCGTGGTCGGC
CGGTCCGCCTCGACGCTGACCGAGACCGCCCGGGGCTACCCGAACATCCATACCCTGCCC
GTCGACATCACCGAGCCGGGAGCGGCCGAGACGATCGTGCGTGCGGCGCTCCGGGAGTTC
GGCCGGATCGACGTCCTGGTGAACAACGCCTCCACCGCCGTGCCGGCACCGCTGGAGAAC
ATCGCGCCGGCGGAGGTCGACCGGCTGCTGGCCGTCAATCTGCGTGCGCCGATCGCTCTC
ACCCGGGAAGCGCTGGACGCGCTGACCAGGACCCGCGGCACCGTGGTGAATCTCAGCAGC
GCCGGCTCCCTCGGCCGGCGTGCCTGGCCGAACTTCTCGCTCTACGGATCCAGCAAGGTC
GCCTTGGACTTCCTCACCCGGACCTGGGCCGTGGAACTGGCTCCCCGCGGAATCCGCGTG
GTCTCCGTCGCCCCGGGGGTGATCGAGACGGGCATGGGCATCCGCATGGGCTCCAGCCCC
GAGGAATACGCGCGGTTTCTGGAGTGGATGGAGAAGATCACCCCGCTCGGCCGGGTCGGA
CAGCCCGAGGAGATCGCCTGGTGGGTGGTGCAGGTCGCGGCGCCGGAGGCGGGCTTCGTC
ACGGGCACGGTGCTCGCGGTCGACGGCGGAGCGAGCATCGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[101-112] 4.89999951782359e-09 PR00080 [153-161] 4.89999951782359e-09 PR00080 [175-194] 4.89999951782359e-09 PR00080
PR00080   SDRFAMILY
IPR002347 Glucose/ribitol dehydrogenase (Family)
[31-48] 3.70001063537244e-36 PR00081 [101-112] 3.70001063537244e-36 PR00081 [147-163] 3.70001063537244e-36 PR00081 [175-194] 3.70001063537244e-36 PR00081 [196-213] 3.70001063537244e-36 PR00081 [235-255] 3.70001063537244e-36 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[30-273] 8.60000000000004e-75 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Stref_00090 Stref_00090   Stref_00110 Stref_00110
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