Stref_00220 Stref_00220   Stref_00240 Stref_00240

Stref_00230 : CDS information

close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction24,050 / 22,887 / - [in whole cluster]
24,050 / 22,887 / - [in contig]
Locationcomplement(22887..24050) [in whole cluster]
complement(22887..24050) [in contig]
TypeCDS
Length1,164 bp (387 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative oxidoreductase
Product (GenBank)putative oxygenase
Gene
Gene (GenBank)stfOII
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
comment
steffimycin生合成gene clusterのクローニング、特徴づけ。
36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連する。

stfOII : Monooxygenase

配列解析のみ。
aglyconeがglycosylationされた後の8-methoxy基の導入は、StfOII and StfMIII によってされそう。
stfOII はmethyl基導入に先立つmonooxygenaseであると提唱されている。
この反応は提唱経路で最終stepに当たる。
Related Reference
ACC
P0ABR5
PmId
[9603882] Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12. (J Bacteriol. , 1998)
[9205837] ( , )
comment
Blast 239th, id34%, 4e-27, N末部分hit
E.coli_hcaE(hcaA, hcaA1 etc.)
3-phenylpropionate/cinnamic acid dioxygenase subunit alpha(EC 1.14.12.19)
synonym: Digoxigenin subunit alpha

---
UniProt function(ref.5=[PMID: 9603882](1998));
multicomponent 3-phenylpropionate dioxygenaseの一部。

3-phenylpropionic acid (PP) → 3-phenylpropionate-dihydrodiol (PP-dihydrodiol)
cinnamic acid (CI) → cinnamic acid-dihydrodiol (CI-dihydrodiol)

---
[PMID: 9205837](1997)
3-phenylpropionic acid (PP)の初期代謝に関与するdioxygenolytic pathwayをコードする遺伝子群として、 hca clusterを同定している。

hcaA1A2CBDのうち、hcaA1A2CDが3-phenylpropionate dioxygenaseをコードし、hcaBがcis-dihydrodiol dehydrogenaseをコードすることが示唆されている。また、hcaTがpermease, hcaRがregulatorをコードすることが推測されている。
Family/Domain
FD
IPR017941
comment
[IPR017941] Rieske [2Fe-2S] iron-sulphur domain (Domain)
function; oxidoreductase activity, 2 iron, 2 sulfur cluster binding

close this sectionSequence

selected fasta
>putative oxidoreductase [putative oxygenase]
MGHSLDRAVPGDVTSPPSTADLLEELRGYLRADPPAISLPPRLFTSPDVYESERARIFGR
SWMMVAHRDQLAQPGDYLALEIAGEPVVITRGDDGMLHGMSPVCRHRMMPLVEPGSGNTQ
DFTCPYHLWRYRLDGTLLAATHMRGNADFDPGQCRLPAFAVEEWRGFVFVNLQADASTLE
DHLTGVDGDLANYRLDDMVQISTWTEVWRCNWKVAVENSYENYHAIGFHPNTVKPLMTGG
IDMEVHADSPLVTRLLTRTGEPMEPSVLPLTEEEKRVLYSFRMFPCSSLATFGESIAWIS
LIPLSIDRTEVRGGTLMPAAALEGADVEAIRKQTEQFTGVINTEDRLGLEAVQRTLGSRF
IERGHLSPKEPGVLAFYRNLALALAAD
selected fasta
>putative oxidoreductase [putative oxygenase]
ATGGGCCATTCCCTCGACCGGGCGGTACCGGGCGACGTCACGTCACCACCGTCGACCGCG
GACCTGCTGGAGGAGCTGCGCGGCTATCTCCGGGCGGATCCTCCCGCGATCAGCCTGCCG
CCTCGGCTGTTCACCTCACCCGACGTCTACGAATCCGAACGCGCCCGGATCTTCGGCCGC
TCCTGGATGATGGTGGCGCACCGGGACCAGCTGGCGCAGCCCGGTGACTACCTCGCACTC
GAGATCGCCGGCGAGCCGGTGGTGATCACCCGGGGCGACGACGGCATGCTGCACGGCATG
TCACCGGTCTGCCGGCACCGCATGATGCCGCTGGTCGAACCCGGTTCGGGCAACACCCAG
GACTTCACCTGCCCCTATCACTTGTGGCGCTACCGCCTCGACGGCACGCTCCTGGCCGCC
ACGCACATGCGGGGCAACGCCGACTTCGATCCCGGACAGTGCCGGCTGCCCGCGTTCGCC
GTGGAGGAATGGCGGGGGTTCGTCTTCGTCAACCTCCAGGCCGACGCCTCGACGCTGGAG
GACCATCTAACCGGCGTGGACGGGGACCTGGCCAACTACCGACTCGACGACATGGTCCAG
ATCTCCACCTGGACCGAGGTCTGGCGGTGCAACTGGAAGGTCGCGGTGGAGAACAGCTAC
GAGAATTACCACGCGATCGGTTTCCATCCCAACACGGTGAAACCGCTCATGACCGGCGGC
ATCGACATGGAGGTGCACGCCGACTCGCCCCTGGTCACCCGGCTGCTCACCCGGACCGGC
GAGCCGATGGAGCCCTCGGTCCTGCCGCTGACCGAGGAGGAGAAGAGGGTGCTGTACAGC
TTCCGGATGTTTCCGTGCAGCAGCCTGGCGACCTTCGGCGAGAGCATCGCCTGGATCAGT
CTCATCCCGCTGTCCATCGACCGGACGGAGGTGCGCGGCGGGACGCTGATGCCGGCGGCG
GCGCTCGAGGGCGCCGATGTGGAGGCGATCCGTAAGCAGACCGAGCAGTTCACGGGTGTG
ATCAACACCGAGGACCGGCTCGGCCTGGAGGCCGTCCAGCGGACGCTCGGCTCCCGGTTC
ATCGAACGGGGACACCTCAGCCCCAAGGAGCCCGGGGTGCTGGCCTTCTACCGCAACCTC
GCCCTGGCGCTGGCAGCGGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001663 Aromatic-ring-hydroxylating dioxygenase, alpha subunit (Domain)
[46-71] 2.90000354677981e-27 PR00090 [76-91] 2.90000354677981e-27 PR00090 [97-117] 2.90000354677981e-27 PR00090 [118-138] 2.90000354677981e-27 PR00090 [159-185] 2.90000354677981e-27 PR00090 [196-218] 2.90000354677981e-27 PR00090
PR00090   RNGDIOXGNASE
IPR015879 Aromatic-ring-hydroxylating dioxygenase, alpha subunit, C-terminal domain (Domain)
[195-385] 1.8e-28 PF00848
PF00848   Ring_hydroxyl_A
IPR017941 Rieske [2Fe-2S] iron-sulphur domain (Domain)
[62-170] PS51296
PS51296   RIESKE
[61-155] 5.30000000000001e-18 PF00355
PF00355   Rieske
[22-175] 4.90001027782408e-46 SSF50022
SSF50022   Rieske_dom
[60-169] 4.70000000000003e-35 G3DSA:2.102.10.10
G3DSA:2.102.10.10   Rieske_reg
SignalP
[1-38] 0.044 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Stref_00220 Stref_00220   Stref_00240 Stref_00240
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