Stref_00240 : CDS information

Location

Organism	Streptomyces steffisburgensis
Strain	NRRL 3193 (=NBRC 13446)
Entry name	Steffimycin
Contig	AM156932
Start / Stop / Direction	25,243 / 24,068 / - [in whole cluster] 25,243 / 24,068 / - [in contig]
Location	complement(24068..25243) [in whole cluster] complement(24068..25243) [in contig]
Type	CDS
Length	1,176 bp (391 aa)

Annotation

Category	3.2 modification methylation
Product	putative O-methyltransferase
Product (GenBank)	O-methyl transferase
Gene
Gene (GenBank)	stfMII
EC number	2.1.1.-
Keyword
Note
Note (GenBank)
Reference	ACC Q2P9Z1 PmId [16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006) comment steffimycin生合成gene clusterのクローニング、特徴づけ。 36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連する。 stfMII : O-Methyltransferase 配列解析。 StfMII はdeoxysugar L-nogaloseのメチル化に関与するSnogYと近しいhomologueなので、 L-rhamnoseでのC-2' hydroxy基のメチル化を担うmethyltransferaseでありうる。
Related Reference	ACC O54261 NITE Nogl_00410 PmId [22120896] Identification of late-stage glycosylation steps in the biosynthetic pathway of the anthracycline nogalamycin. (Chembiochem. , 2012) comment Blast 3rd, id55%, 7e-97 Streptomyces nogalater_snogY SnogY [DoBISCUIT]demethoxynogalose C-2' O-methyltransferase_snogY aglycone生合成とdeoxysugar形成に必要な29ORFsを含むpSnogaoriを異種ホストS. albusに導入。・3',4'-demethoxynogalose-1-hydroxynogalamycinone ・nogalamineがC-C結合ありrhodosamineで置換されたnogalamycin R ・nogalamineがC-C結合なし2-deoxyfucoseで置換されたnogalamycin F がS. albus/pSnogaoriで産生された。 sno clusterにあるO-methyltransferase候補のうち、pSnogaoriにはsnogYが含まれるが、snogM and snogLは含まれない。 S. albus/pSnogaoriの産物はどれもnogalose部分のC2'にmethoxy基を含むので、SnogYがC2'でのO-methylationを担う。提唱経路schemeでは、aglyconeに付着されてからメチル化を受けるとされている。
Related Reference	ACC Q2P9Y7 NITE Stref_00280 PmId [16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006) comment Blast 18th, id42%, 2e-66 Streptomyces steffisburgensis_stfMIII 同じstf cluster内にあるhomolog 配列解析によりmethyltransferase候補とされ、他の候補であるStfMI, StfMII の機能推定からの消去法で8-hydroxyl基のO-methylationが割り当てられている。

Sequence

>putative O-methyltransferase [O-methyl transferase]
MSAESDLLDRVIRAGTASDAEISELVEEAGLDAVIGVLVDEVLFRCDEPVNATPVDIALD
LTHGTERRRTVFRIVRDEPIEIVDAEEPVIRRELRMDVRDLVRRLYGRADHRRAGDFYDT
FLPTRPEHLWELPAILAATNQASGTLLSGCTVPHVDLGALSVAHGSDKWASFHWYTAHYQ
KEFAPYRDQPVRLLEIGIGGFEGELGGPSLKMWKRYFHRGAVFGLDLFDKSALNQPRLTA
LVGDQGDTETLVAIAEEHGPFDIVIDDGSHENEHVRTSFDSLFPYVRSGGLYVIEDLQTS
YFPRFGGTAGDEAGPQTSVGLLKRLLDDLHYQEQEARTDEPPTVTQDAVVGVRVYRNIAF
IEKGVNGENGIPRWMDDEAWVALGAIPPAAE

>putative O-methyltransferase [O-methyl transferase]
ATGTCCGCTGAGAGTGACCTGCTCGACCGCGTGATCCGGGCGGGGACCGCTTCCGACGCG
GAGATCTCCGAGCTGGTCGAGGAGGCCGGACTCGACGCGGTGATCGGTGTTCTCGTCGAC
GAAGTGCTGTTCCGTTGCGACGAACCGGTCAACGCGACACCCGTCGACATCGCGCTGGAC
CTCACGCACGGGACCGAACGGCGACGGACCGTCTTCCGCATCGTCCGGGACGAGCCCATC
GAGATCGTCGACGCCGAGGAGCCGGTGATCCGGCGCGAACTGCGCATGGACGTCAGGGAC
CTGGTGCGCCGGCTGTACGGGCGGGCGGACCATCGTCGTGCCGGGGACTTCTACGACACC
TTCCTGCCCACCCGCCCCGAGCATCTGTGGGAGCTGCCGGCGATCCTCGCGGCGACCAAC
CAGGCCTCCGGCACACTGCTCTCCGGCTGCACCGTCCCCCACGTCGACCTCGGGGCGCTG
TCCGTCGCCCACGGATCGGACAAGTGGGCCAGCTTCCACTGGTACACGGCCCACTACCAG
AAGGAGTTCGCCCCATACCGGGACCAGCCCGTGCGGCTGCTCGAGATCGGGATCGGCGGC
TTCGAAGGGGAACTCGGCGGACCGTCCCTGAAGATGTGGAAGAGGTACTTCCACCGCGGT
GCAGTCTTCGGTCTCGACCTGTTCGACAAGTCCGCCCTGAACCAGCCCAGGCTGACCGCG
CTGGTCGGCGATCAGGGCGACACCGAAACCCTGGTCGCCATCGCGGAAGAGCACGGCCCC
TTCGACATCGTCATCGACGACGGCAGTCACGAGAACGAGCATGTGCGCACCTCGTTCGAC
TCGCTCTTCCCCTACGTGCGCAGCGGTGGCCTCTACGTCATCGAGGACCTGCAGACCTCG
TACTTCCCGAGGTTCGGGGGAACGGCGGGTGACGAGGCCGGACCGCAGACCTCGGTGGGG
CTGCTCAAGCGGCTGCTGGACGACCTGCATTACCAGGAACAGGAAGCGCGGACCGACGAG
CCCCCGACCGTGACCCAGGACGCCGTGGTCGGTGTACGGGTCTATCGGAACATCGCGTTC
ATCGAGAAGGGCGTCAACGGGGAGAACGGCATCCCCAGGTGGATGGACGACGAGGCCTGG
GTGGCCCTGGGCGCGATACCCCCGGCGGCGGAGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Eukaryota
TMHMM	No significant hit

Stref_00230 Stref_00250