Stref_00240 Stref_00240   Stref_00260 Stref_00260

Stref_00250 : CDS information

close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction25,574 / 25,233 / - [in whole cluster]
25,574 / 25,233 / - [in contig]
Locationcomplement(25233..25574) [in whole cluster]
complement(25233..25574) [in contig]
TypeCDS
Length342 bp (113 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.2 function unknown
Productmuconolactone delta-isomerase family protein
Product (GenBank)putative 5-chloromuconolactone dehalogenase
Gene
Gene (GenBank)stfF
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
comment
steffimycin生合成gene clusterのクローニング、特徴づけ。
36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連する。

stfF: Unknown

配列解析のみ。
muconolactone delta-isomerases (pfam02426)に属する。
5-chloromuconolactone → cis-dienelactone への変換をする
5-chloromuconolactone dehalogenase_ClcFに似ている。
Related Reference
ACC
Q8G9L0
PmId
[12957954] ( , )
comment
Blast 3rd, id39%, 5e-05, N末alignmentイマイチ、C末gapあり
Rhodococcus opacus(Nocardia opaca)_clcF
5-chloromuconolactone dehalogenase

---
[PMID: 12218013](2002)
産物のHPLC解析などから、3-chlorocatechol分解において、
ClcFが5-chloromuconolactone → cis-dienelactoneを担う5-chloromuconolactone dehalogenaseであることを確認。
muconolactone isomerasesに配列が似ているが、muconolactone-isomerizing activityを示さない。

---
[PMID: 12957954](2003)
(19)F NMRを使って、ClcFは5-fluoromuconolactone → cis-dienelactoneへのdehalogenationを触媒することを確認。

close this sectionSequence

selected fasta
>muconolactone delta-isomerase family protein [putative 5-chloromuconolactone dehalogenase]
MALFAVLATQAPAAGEREEFLRRLPEGFAYTKDLVDKGVIRHSWIRVGASGGLNIYDVDS
HEELLSVLYSNPVSPHLTFEVIPLAVPESFDPAAYLLKWNKKDGSRDEARHVR
selected fasta
>muconolactone delta-isomerase family protein [putative 5-chloromuconolactone dehalogenase]
ATGGCGCTGTTCGCGGTACTCGCCACACAGGCACCGGCAGCGGGAGAACGGGAGGAATTC
CTGCGCCGGCTTCCCGAAGGATTCGCCTACACGAAAGACCTGGTGGACAAAGGTGTGATC
CGGCACAGCTGGATCCGGGTCGGCGCGTCCGGCGGTCTGAACATCTACGACGTCGATTCG
CACGAGGAGTTGCTCTCCGTGCTCTACAGCAACCCGGTCAGTCCGCATCTGACCTTCGAG
GTCATTCCCCTGGCCGTTCCGGAGAGCTTCGACCCGGCGGCCTATCTGCTCAAGTGGAAC
AAGAAGGACGGTTCCAGGGATGAGGCCCGGCATGTCCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR011008 Dimeric alpha-beta barrel (Domain)
[1-96] 1.10000150671642e-20 SSF54909
SSF54909   Dimer_A_B_barrel
IPR026029 Muconolactone isomerase domain (Domain)
[29-85] 1.1e-08 PF02426
PF02426   MIase
SignalP
[1-15] 0.955 Signal
Eukaryota   
TMHMM No significant hit
Stref_00240 Stref_00240   Stref_00260 Stref_00260
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