Stref_00310 Stref_00310   Stref_00330 Stref_00330

Stref_00320 : CDS information

close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction37,308 / 36,061 / - [in whole cluster]
37,308 / 36,061 / - [in contig]
Locationcomplement(36061..37308) [in whole cluster]
complement(36061..37308) [in contig]
TypeCDS
Length1,248 bp (415 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note
  • This ORF is not involved in steffimycin biosynthesis.
Note (GenBank)
  • ORF32
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
comment
steffimycin生合成gene clusterのクローニング、特徴づけ。

36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連し、一次代謝関連genesとhousekeeping genesを含む12ORFsによって挟まれている。

orf29-32は類似性のあるタンパクなし。

orf31-36を含まない異種性発現株S.albus strain STFaRHAMでsteffimycinの生合成ができている。

close this sectionSequence

selected fasta
>hypothetical protein [hypothetical protein]
MLRTLEEELVAGLRAFDGACAAAAGEAPAVTPETFDAVDSAARAVEALLRARPPLGAWFN
REFDRLVEGRERFGPAAAPTYWTSYLRSLLVPVMYATDRAFEVSGAGAVTYGSGRGELSY
GRATVSVPDERPAGTAGKPRWWRLQFRPDPGRDVLLGAIEPSGRGAFAEQVRTQLDDGAD
REALVFVHGFNVAFADAARQAAQIAYDLNFTGVVLLYSWASKGRLLDYPADGDAVRRAVP
YFQDFLRTLLTRTGVREVHIMAHSMGNRLLADGLAGLDTEALPEGSGRLGHVVFAAPDVD
RDVFAHLLPSVLRQARTCTLYVSDRDRALSAARKLSDFPRAGQSGTSVIVAPGLDTIDVT
ELGADLLGHSYMANHTTVLGDLYGLFRHGHAPAERYGLARTPHPDGDYWSFAPQK
selected fasta
>hypothetical protein [hypothetical protein]
GTGCTGAGGACTTTGGAAGAGGAGTTGGTCGCCGGGCTGCGCGCCTTCGACGGGGCGTGC
GCGGCAGCCGCCGGCGAAGCACCCGCCGTGACTCCCGAAACCTTCGACGCCGTGGACAGC
GCGGCTCGCGCGGTGGAGGCCTTGCTACGGGCTCGACCGCCTCTGGGCGCATGGTTCAAC
CGGGAGTTCGACCGGCTCGTCGAAGGGCGGGAGCGCTTCGGTCCGGCCGCGGCACCGACC
TACTGGACCTCTTATCTGCGCTCGCTCCTTGTTCCCGTCATGTATGCCACCGACCGCGCA
TTCGAGGTTTCCGGCGCGGGTGCGGTGACGTACGGGTCCGGTCGGGGCGAACTCTCCTAT
GGCCGGGCCACGGTGAGTGTGCCGGACGAGCGTCCGGCGGGTACCGCGGGCAAACCCCGT
TGGTGGCGGCTGCAGTTCCGTCCGGACCCGGGACGGGACGTGCTGCTCGGAGCGATCGAA
CCGTCGGGCCGCGGTGCCTTCGCCGAACAGGTGCGCACGCAGCTCGACGACGGTGCTGAC
CGCGAGGCTCTGGTGTTCGTGCACGGATTCAACGTGGCCTTCGCCGACGCCGCCCGACAG
GCCGCGCAGATCGCCTACGATCTCAACTTCACCGGGGTGGTCCTTCTCTACAGCTGGGCG
TCCAAGGGGCGCCTGCTGGACTACCCCGCCGACGGGGACGCCGTGCGCCGGGCCGTCCCT
TACTTCCAGGACTTCCTGCGGACCCTGCTCACCCGGACAGGTGTGCGTGAAGTCCACATC
ATGGCCCACAGTATGGGAAACCGGCTGCTCGCCGACGGGCTGGCGGGTCTGGACACCGAG
GCGCTTCCGGAGGGATCCGGACGGCTGGGCCACGTCGTGTTCGCCGCGCCGGACGTCGAC
CGTGACGTCTTCGCGCACCTGCTGCCCAGCGTGCTCCGCCAGGCCCGCACCTGCACGCTC
TACGTCTCGGACCGTGATCGAGCTCTGTCCGCCGCCCGGAAGCTGTCGGACTTCCCGCGT
GCGGGCCAGAGCGGCACCTCGGTGATCGTCGCCCCGGGACTGGACACCATCGACGTGACC
GAACTGGGCGCCGACCTGCTGGGCCACTCGTACATGGCCAACCACACGACCGTGCTCGGC
GATCTGTACGGACTGTTCCGGCACGGCCACGCCCCGGCAGAGCGTTACGGGCTGGCCCGC
ACACCTCATCCGGACGGCGACTACTGGTCGTTCGCGCCCCAGAAATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR010297 Protein of unknown function DUF900, hydrolase-like (Family)
[166-382] 3.40000000000004e-52 PF05990
PF05990   DUF900
SignalP No significant hit
TMHMM No significant hit
Stref_00310 Stref_00310   Stref_00330 Stref_00330
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