Stref_00300 Stref_00300   Stref_00320 Stref_00320

Stref_00310 : CDS information

close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction36,010 / 34,976 / - [in whole cluster]
36,010 / 34,976 / - [in contig]
Locationcomplement(34976..36010) [in whole cluster]
complement(34976..36010) [in contig]
TypeCDS
Length1,035 bp (344 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)putative trypsin-like serine protease
Gene
Gene (GenBank)
EC number
Keyword
Note
  • This ORF is not involved in steffimycin biosynthesis.
Note (GenBank)
  • ORF31
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
comment
steffimycin生合成gene clusterのクローニング、特徴づけ。

36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連し、一次代謝関連genesとhousekeeping genesを含む12ORFsによって挟まれている。

orf29-32は類似性のあるタンパクなし。

orf31-36を含まない異種性発現株S.albus strain STFaRHAMでsteffimycinの生合成ができている。

close this sectionSequence

selected fasta
>hypothetical protein [putative trypsin-like serine protease]
MTHFVDRVPFQWDAPGALELRDLLAGVYTRSAPAEQLAKRAGVATGYINWEQPMMTVWFE
LMEKAANQKCLRPLLEAVTEGPDQAVADRVRELLAAQPVVEAPDRPTGPQDFAEFDDPDQ
LERQIFRSHTLQDVAFLRRGTELATSVCRLRVTAADGSQYHGTAFRITDDLLLTNHHVLF
DGNGSAPLGVEAWFGYEAELNGRTREHQVVLCDVESAVGEPELDWAVVRSAEPLPGDALR
VALPSAVHVSLNDRVYIIQHPHGGVKKLAARHNVVRHVDDTVVQYWTDTEQGSSGSPVFD
ERWNLVALHRRWLRVGADDEPAEFRNEGVRIERVLDGLARKRLV
selected fasta
>hypothetical protein [putative trypsin-like serine protease]
ATGACGCACTTCGTGGACCGAGTTCCCTTCCAGTGGGATGCGCCCGGCGCCCTGGAACTG
CGTGATCTGCTGGCCGGGGTGTACACCCGATCCGCCCCGGCCGAGCAACTGGCGAAGCGG
GCCGGTGTGGCGACGGGATACATCAACTGGGAACAGCCCATGATGACCGTCTGGTTCGAA
CTCATGGAGAAAGCGGCCAACCAGAAGTGTCTGCGCCCCCTTTTGGAGGCGGTCACCGAG
GGGCCTGACCAGGCTGTCGCCGACCGGGTCCGTGAACTCCTCGCCGCGCAGCCTGTCGTG
GAGGCACCGGACAGGCCCACGGGGCCGCAAGATTTCGCGGAGTTCGACGATCCCGACCAA
CTGGAGCGGCAGATCTTCCGCTCCCACACGCTCCAGGACGTGGCCTTCCTGCGCCGGGGA
ACCGAACTGGCCACTTCCGTGTGCCGGTTGCGCGTCACAGCCGCCGACGGCAGTCAATAC
CACGGCACGGCCTTCCGTATCACGGACGATCTTCTACTGACCAATCACCATGTGCTGTTC
GACGGCAACGGAAGCGCACCGCTCGGGGTCGAGGCGTGGTTCGGTTACGAGGCCGAGCTG
AACGGGCGGACGCGCGAGCATCAAGTGGTGCTCTGTGACGTGGAGTCGGCTGTGGGAGAG
CCCGAGCTGGACTGGGCGGTCGTCCGGAGCGCCGAGCCGCTGCCCGGTGACGCCCTTCGT
GTCGCCCTGCCCAGCGCTGTCCACGTGTCCCTGAACGACAGGGTGTACATCATCCAGCAC
CCGCACGGCGGGGTGAAGAAGCTCGCCGCCCGCCACAATGTCGTACGCCATGTCGACGAC
ACTGTGGTGCAGTACTGGACCGACACGGAACAGGGATCGTCCGGGTCGCCCGTCTTCGAC
GAGCGGTGGAACCTCGTCGCCTTGCACCGCCGCTGGCTCAGGGTCGGCGCCGACGACGAG
CCCGCCGAGTTCCGTAACGAAGGTGTGAGGATCGAGCGGGTTCTGGATGGACTCGCACGG
AAGCGACTGGTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR008256 Peptidase S1B, glutamyl endopeptidase I (Domain)
[162-179] 1.30000067202732e-06 PR00839 [283-299] 1.30000067202732e-06 PR00839
PR00839   V8PROTEASE
IPR009003 Peptidase cysteine/serine, trypsin-like (Domain)
[146-339] 4.40000933643891e-28 SSF50494
SSF50494   Pept_Ser_Cys
SignalP No significant hit
TMHMM No significant hit
Stref_00300 Stref_00300   Stref_00320 Stref_00320
Page top