Stref_00290 Stref_00290   Stref_00310 Stref_00310

Stref_00300 : CDS information

close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction34,971 / 33,847 / - [in whole cluster]
34,971 / 33,847 / - [in contig]
Locationcomplement(33847..34971) [in whole cluster]
complement(33847..34971) [in contig]
TypeCDS
Length1,125 bp (374 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in steffimycin biosynthesis.
Note (GenBank)
  • ORF30
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
comment
steffimycin生合成gene clusterのクローニング、特徴づけ。

36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連し、一次代謝関連genesとhousekeeping genesを含む12ORFsによって挟まれている。

orf29-32は類似性のあるタンパクなし。

orf30を完全には含まない異種性発現株S.albus strain STFaRHAMでsteffimycinの生合成ができている。

close this sectionSequence

selected fasta
>hypothetical protein [hypothetical protein]
MPALIDERSFNLDDERAKNLLKALVTVYSTKPAARQVILGAGLGEGDINWGQSMADVWPE
ALELAAPAGRLRALVQLAANDPNTRGHPVFHYLLTEETSAAETDPCTAYLVANRQRAFFN
RLKFRTTLHHMVEGAGGRVLAVEGERQTGRTHSWYLIAHVLDSHRVNRRRIRMRDFYSPV
EVADIGVTLRELFGWNITLDLNTSEDSQSRSLVNQIKHLLNEEHRTGQVLTGHWLVIDDS
QSVRFTEPALRALIRLVAAVIEEEMADRLRIVLLAYDGWLPSDLESYVAHEHFAPLTGKD
LHDYVRAVAKEAGSPVDSEQADQLVNELSSLSGCGPVTADAPLPMSDRLQLVAASWGRDQ
YDRGGARCDQRSER
selected fasta
>hypothetical protein [hypothetical protein]
ATGCCCGCGTTGATCGACGAGCGCTCCTTCAACCTCGACGACGAACGAGCCAAGAACCTG
CTCAAGGCCCTGGTCACCGTCTACAGCACCAAACCCGCCGCGCGGCAGGTCATTCTCGGC
GCCGGACTGGGTGAGGGCGATATCAACTGGGGTCAGAGCATGGCCGACGTCTGGCCGGAG
GCTCTTGAACTCGCGGCCCCGGCCGGTCGTCTGCGGGCCCTCGTCCAGTTGGCCGCGAAC
GACCCCAACACCCGGGGCCATCCGGTCTTTCACTACCTTCTGACGGAGGAGACGTCCGCC
GCCGAAACGGACCCGTGCACCGCGTATCTGGTGGCCAACCGCCAAAGGGCATTCTTCAAC
CGCCTGAAGTTCCGGACCACGCTGCACCACATGGTGGAAGGGGCTGGCGGACGTGTCCTC
GCCGTGGAGGGAGAGCGTCAGACAGGCAGGACACACAGCTGGTACCTCATCGCCCATGTA
CTGGACAGCCATCGCGTCAACAGGCGACGCATCAGGATGCGCGACTTCTACTCACCGGTG
GAGGTCGCCGACATAGGCGTCACCCTGAGGGAACTCTTCGGGTGGAACATCACGCTCGAT
CTCAACACCTCGGAGGACAGCCAGTCCCGCAGTCTGGTCAACCAGATCAAGCACCTGCTG
AACGAGGAACACCGGACGGGGCAGGTGCTGACCGGGCACTGGCTCGTCATCGACGACTCT
CAGTCCGTGCGCTTCACCGAGCCCGCCCTGAGAGCGTTGATCCGGCTGGTGGCCGCCGTG
ATCGAGGAGGAGATGGCGGACCGGCTCCGCATCGTCCTGCTCGCCTACGACGGCTGGCTC
CCCTCCGACCTGGAGTCCTACGTCGCCCACGAGCACTTCGCCCCGCTCACCGGCAAGGAC
CTGCATGACTACGTCCGGGCCGTCGCCAAGGAAGCAGGCAGTCCCGTCGACTCCGAACAG
GCCGACCAGCTCGTCAACGAACTGAGCAGCCTGTCCGGCTGCGGCCCCGTCACCGCCGAC
GCTCCGCTGCCGATGTCCGACCGGCTGCAACTCGTCGCCGCGTCATGGGGCCGTGACCAG
TACGACAGGGGAGGTGCCCGTTGTGACCAGCGCTCCGAACGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
Stref_00290 Stref_00290   Stref_00310 Stref_00310
Page top