Stref_00290 : CDS information

Location

Organism	Streptomyces steffisburgensis
Strain	NRRL 3193 (=NBRC 13446)
Entry name	Steffimycin
Contig	AM156932
Start / Stop / Direction	33,797 / 29,583 / - [in whole cluster] 33,797 / 29,583 / - [in contig]
Location	complement(29583..33797) [in whole cluster] complement(29583..33797) [in contig]
Type	CDS
Length	4,215 bp (1,404 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in steffimycin biosynthesis.
Note (GenBank)	ORF29
Reference	ACC Q2P9Y6 PmId [16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006) comment steffimycin生合成gene clusterのクローニング、特徴づけ。 36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連し、一次代謝関連genesとhousekeeping genesを含む12ORFsによって挟まれている。 orf29-32は類似性のあるタンパクなし。 orf29はsteffimycin生合成には関係ないとされる12ORFsに含まれるが、異種性steffimycin発現のためのplasmid pEM4STFaには含まれている。

Sequence

>hypothetical protein [hypothetical protein]
MLLGSFAAQDLVPDGDHETAGPSAALQDFLLDECTRISTPRGRRWRLADGSRTRTLEQLR
TRERLLAAARNAPRRDDPARAWAERLLRGENPPLEEQSFEELYTALTVVGWFRDAPVSRK
ALVHDGVRLPDLDDLESAVKRARLFQPLRALADARFTGRTTELALLNAVFEGARDTSESA
QVPASALSVGSQELSTWTFVHGPGGVGKSTLLARFVLDRLDGRRSVPVDERTSSQDRERR
GSSPCFYLTFDRHDLVAERPLTLVAESVRQLGLLRPDLAQRTAELERELEITLSADWFTR
SEDSHHGPRAAHQRDELTLIDAFAALVASITGGTDVSQPWLLALDAFEQVQRGGPVALRR
LLDFLDGLRRAHPGLRVLAAGRVPVAEPAFRPLRLDGFDAVTARAFLRRELADAPSVPPA
HGQFTAGDQDIEEIIGIVGTSPLNLKLAAALVHREGVRFLGEGDLPAELRLKLSAETVQG
VLYRRLLDHFHDRDLRRIASPGLVVRVLTPDVIREVLAVPCGLGRLDEDRARRMYYAFRA
EATLVEDVPGQEAVVHRGDVRRAMLPLLRRDHRTVVERIHRKAVRYYARTDAHTDPVASR
VEELYHRLALGQSTRTLDGRWIEDAGPLLESAIEELPASAQVYLIEKLGGSAPDDLRAAG
DDEVWERQALRIGKALLAADDPAGVTNLLGERPHRVSGNLDLTALSMRAALALRRPVDAY
DLVGGAMDLAADTADPEVFVELTLLGARSCEDLGRFEEALDLLGRARRVADEPGMVTRLL
SVAAAQLRIHRRSGTAATAEATALRAETVRRVRALSSKALRRHPQLIRELAAEIGDELPH
LVSYTARSLGVGGTEGADDALLGSLTGEVGVVPRVPAGTAGTISVGSVGRGYAVGDYLDT
HADTADPWNEALVDTYRHELDRPYTGDAVVVLPGFAGSALVEGETGEAVWGTGVASQQLL
TPVDLFRDLERLRVTAAERAGDLVMLRPTGLLREVAWLPLVGGLSPYGALVKGVNSSVLH
DRAVLEFPYDWRLSVAAAAPHLAKAALRHLAQWRDHPARRASVLSRVGEGPKLVLVAHAM
GGLVAQAALAEFPELARATREVVTIGTPFQGIPKIMQFMDPFGSPLRHVSVARPLAEAAR
SMPGLYDLLPDTRCVYAGHGGMRRLTLRDVEEVGGDWALAADAFEARLRRQAAVGRLPRW
HAIVGTSQPTAQGMSIEGGRVRLLPLTTKVDGEGNYVRDEDDRLIAVQGGGDGLVPLGSA
VPSGFAECLPVVGQHSSLLTSSTVVNLVQEIVSGKPASRTLPLSQEAGLRPTSFGISAPP
EVTPGDQWTLTVTGADHAALSCSLVRATTGEVIARIRTAPVEGRPGTSTARTTVREPGFY
QVVARAGGLSATALVVAVQYATTD

>hypothetical protein [hypothetical protein]
GTGCTCCTGGGATCGTTCGCGGCTCAGGACCTCGTGCCGGACGGTGACCACGAGACAGCT
GGGCCCAGCGCCGCCCTCCAGGACTTTCTGCTGGACGAGTGCACCCGCATCAGTACGCCC
CGGGGCCGCCGGTGGCGCCTAGCGGACGGTTCCCGTACCCGGACCCTGGAGCAGCTGCGC
ACCCGGGAGAGGCTGCTGGCCGCGGCGCGGAACGCGCCCCGCCGTGACGACCCGGCTCGC
GCCTGGGCCGAACGCCTGCTGAGAGGCGAAAATCCGCCGCTGGAGGAGCAGAGCTTCGAG
GAGCTGTACACGGCGCTGACTGTCGTGGGCTGGTTCCGTGACGCCCCGGTGTCCCGGAAG
GCGCTGGTACACGACGGAGTCAGGCTTCCGGACCTCGACGACCTGGAGAGCGCGGTCAAG
AGGGCACGGCTCTTCCAGCCGCTGCGTGCTCTCGCAGACGCCAGGTTCACCGGCCGCACC
ACCGAACTCGCGTTACTCAACGCAGTCTTCGAAGGTGCCAGAGATACTTCGGAATCCGCC
CAGGTCCCCGCCTCAGCGCTGTCCGTCGGCAGCCAGGAGCTCAGCACCTGGACGTTCGTC
CACGGACCCGGCGGTGTCGGCAAGTCCACTCTCCTCGCACGGTTCGTGCTGGACCGCCTC
GACGGACGGCGTTCCGTGCCTGTGGATGAACGGACTTCGTCGCAAGACCGGGAGCGACGT
GGGTCGTCGCCCTGCTTCTACCTCACCTTCGATCGGCACGACCTGGTTGCCGAGAGGCCG
CTGACCCTGGTCGCCGAATCCGTACGGCAACTGGGTCTGCTCCGTCCGGACCTGGCCCAA
CGCACGGCGGAGCTGGAGCGGGAGCTGGAGATCACGCTCTCCGCCGACTGGTTCACCCGC
TCCGAGGACAGTCACCACGGTCCGCGGGCCGCCCACCAGCGCGACGAACTGACCCTGATC
GACGCGTTCGCCGCGTTGGTCGCCTCGATCACCGGTGGGACCGATGTCTCCCAGCCGTGG
CTGTTGGCGCTGGACGCGTTCGAACAGGTGCAGCGCGGCGGACCGGTCGCCCTGCGCCGT
CTGTTGGACTTCCTCGACGGGTTGCGCCGGGCGCACCCGGGGCTGAGGGTCCTCGCGGCC
GGCCGTGTGCCTGTCGCCGAACCCGCCTTCAGGCCGCTGCGGCTGGACGGGTTCGACGCC
GTCACCGCGCGTGCCTTCCTCCGTCGTGAACTCGCCGACGCTCCGTCGGTCCCACCTGCA
CATGGCCAGTTCACGGCGGGTGACCAGGACATCGAGGAGATCATCGGCATCGTCGGTACC
AGTCCGCTCAACCTGAAGCTCGCCGCCGCCCTCGTCCATCGGGAAGGGGTGCGCTTCCTC
GGCGAGGGGGACCTGCCGGCAGAACTCCGGCTGAAACTCAGTGCGGAGACGGTGCAGGGT
GTGCTCTACCGGCGTCTGCTGGATCATTTCCATGACCGGGACCTGCGCAGGATCGCGAGC
CCCGGCCTCGTGGTCCGGGTCCTCACCCCTGACGTCATCCGGGAGGTGCTGGCCGTCCCG
TGCGGCCTCGGTCGTCTGGACGAGGACCGGGCTCGGCGGATGTACTACGCCTTCCGGGCC
GAGGCCACGTTGGTGGAGGACGTGCCGGGCCAGGAGGCTGTCGTGCATCGCGGTGACGTC
CGCCGCGCCATGCTCCCGCTGCTCCGTCGCGACCACCGAACCGTCGTGGAGCGGATCCAC
CGCAAGGCGGTGCGCTACTACGCGCGGACGGACGCGCACACTGACCCCGTGGCGAGCCGT
GTCGAGGAGCTGTACCACCGGCTCGCTTTGGGGCAGTCCACCCGTACCCTGGACGGACGC
TGGATCGAGGACGCGGGCCCGCTGCTGGAGTCGGCCATCGAGGAACTGCCCGCTTCCGCC
CAGGTCTATCTGATCGAGAAACTCGGCGGCTCCGCCCCGGACGACCTGCGTGCGGCGGGG
GACGACGAGGTCTGGGAACGCCAGGCCCTGCGCATCGGCAAGGCTCTCCTGGCTGCGGAC
GATCCCGCCGGTGTCACCAATTTGCTGGGCGAACGCCCTCACCGGGTTTCAGGCAATCTC
GACCTCACCGCGCTGAGCATGCGGGCTGCGCTCGCCCTGCGGCGTCCCGTGGACGCCTAC
GACCTGGTCGGCGGGGCCATGGACCTGGCGGCCGACACAGCCGATCCGGAGGTGTTTGTC
GAGCTGACCTTGCTGGGTGCCCGCAGCTGTGAGGACCTCGGCCGCTTCGAAGAGGCCCTC
GACCTCCTGGGGCGGGCACGCCGGGTCGCCGACGAGCCGGGCATGGTGACCCGTCTGCTG
TCCGTGGCCGCGGCGCAGCTCAGAATCCACCGGCGGTCCGGGACCGCCGCCACCGCAGAG
GCCACCGCCCTGCGTGCCGAGACCGTGAGGAGAGTCAGGGCGCTCTCCTCGAAGGCCCTT
CGACGCCATCCGCAGCTGATCCGGGAACTGGCGGCCGAGATCGGCGACGAGCTGCCGCAT
CTGGTCTCCTACACGGCGCGGTCGCTGGGCGTCGGCGGCACCGAGGGGGCCGATGACGCC
CTGCTCGGTTCGTTGACCGGCGAGGTCGGCGTGGTGCCGCGGGTGCCGGCCGGCACCGCG
GGCACGATCAGTGTCGGCAGTGTGGGCCGGGGATACGCCGTCGGCGACTACCTGGACACG
CACGCCGATACCGCCGACCCGTGGAATGAGGCCCTGGTCGACACCTACCGTCACGAACTG
GACCGGCCCTACACCGGCGACGCCGTCGTCGTCCTGCCCGGATTCGCCGGGAGCGCTCTG
GTGGAAGGGGAAACGGGCGAGGCGGTCTGGGGAACCGGCGTCGCTTCCCAGCAGCTTCTG
ACTCCCGTCGACCTGTTCCGGGACCTGGAACGGCTGCGAGTGACGGCGGCGGAACGCGCC
GGGGACCTGGTCATGCTGCGCCCCACCGGACTGCTGCGGGAGGTCGCCTGGCTCCCTCTG
GTGGGCGGACTGTCGCCCTACGGCGCTCTCGTCAAGGGTGTCAACTCGTCGGTGCTGCAT
GACCGGGCCGTGCTGGAATTCCCCTATGACTGGCGTCTGTCCGTCGCTGCCGCGGCACCG
CACCTTGCCAAGGCCGCGCTGCGACATCTGGCCCAGTGGCGGGACCACCCGGCGCGGCGC
GCATCCGTCCTGAGCCGGGTCGGCGAAGGACCCAAGCTGGTGCTGGTCGCCCATGCGATG
GGCGGCCTGGTCGCCCAGGCCGCCCTGGCGGAGTTCCCGGAGCTTGCGAGGGCGACGCGG
GAGGTCGTCACCATCGGCACACCCTTCCAGGGGATTCCCAAGATCATGCAGTTCATGGAT
CCGTTCGGCTCTCCCCTGAGGCATGTGTCGGTGGCCCGTCCCCTGGCGGAGGCGGCGCGC
TCCATGCCGGGACTGTACGACCTGCTGCCCGACACCCGTTGTGTGTACGCCGGCCACGGC
GGAATGCGCAGGCTCACACTCCGCGACGTCGAGGAGGTGGGAGGGGACTGGGCTCTGGCC
GCGGACGCCTTCGAGGCCCGCCTTCGGCGGCAGGCCGCCGTCGGCCGCCTGCCCCGCTGG
CACGCGATCGTGGGTACGTCGCAGCCAACCGCGCAGGGCATGAGCATCGAGGGCGGTCGA
GTACGGCTGCTGCCCCTCACCACGAAGGTGGACGGCGAGGGGAACTACGTCCGCGACGAG
GACGACAGGCTGATTGCGGTACAGGGGGGAGGCGACGGCCTGGTGCCCCTGGGGTCGGCG
GTCCCCTCCGGATTCGCCGAGTGTCTGCCGGTGGTGGGGCAGCACAGTTCCCTGCTTACC
AGCTCCACGGTCGTCAACCTGGTGCAGGAGATCGTCAGCGGGAAACCCGCGTCCAGAACC
CTTCCGCTCAGTCAGGAGGCCGGTCTTCGGCCCACGTCCTTCGGTATCAGCGCCCCGCCT
GAAGTCACCCCGGGAGACCAGTGGACGCTGACGGTCACGGGCGCGGACCACGCCGCTCTC
TCGTGCAGCCTGGTCCGTGCGACCACCGGCGAGGTGATCGCACGTATCCGGACCGCTCCT
GTCGAGGGCCGGCCCGGTACCTCGACCGCACGGACCACGGTCCGGGAGCCCGGCTTCTAC
CAGGTGGTGGCCCGGGCAGGCGGGCTTTCCGCGACGGCGCTGGTGGTGGCCGTGCAGTAT
GCGACGACCGATTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR012908 GPI inositol-deacylase PGAP1-like (Family) PF07819 PGAP1
SignalP	No significant hit
TMHMM	No significant hit

Stref_00280 Stref_00300