Stref_00330 Stref_00330   Stref_00350 Stref_00350

Stref_00340 : CDS information

close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction40,605 / 39,673 / - [in whole cluster]
40,605 / 39,673 / - [in contig]
Locationcomplement(39673..40605) [in whole cluster]
complement(39673..40605) [in contig]
TypeCDS
Length933 bp (310 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.2 translation
Productputative methionyl-tRNA formyltransferase
Product (GenBank)putative methionyl-tRNA formyltransferase
Genefmt
Gene (GenBank)
EC number2.1.2.9
Keyword
Note
  • This ORF is not involved in steffimycin biosynthesis.
Note (GenBank)
  • ORF34
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
comment
steffimycin生合成gene clusterのクローニング、特徴づけ。

36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連し、一次代謝関連genesとhousekeeping genesを含む12ORFsによって挟まれている。

orf1-4, 33-36は、S. coelicolor A3(2) and S. avermitilis MA-4680由来の関連タンパクと有意な類似性あり。遺伝子構成も同じ。

orf31-36を含まない異種性発現株S.albus strain STFaRHAMでsteffimycinの生合成ができている。
Related Reference
ACC
P23882
PmId
[1624424] ( , )
[8432722] ( , )
[8887566] ( , )
[9843487] ( , )
comment
Blast 243rd, id41%, 9e-53
E.coli_fmt
Methionyl-tRNA formyltransferase(EC EC 2.1.2.9)

配列決定、クローニング、酵素活性測定、立体構造解析を行なっている。

close this sectionSequence

selected fasta
>putative methionyl-tRNA formyltransferase [putative methionyl-tRNA formyltransferase]
MKLVFAGTPEVAVPALDALLASGRHEVAAVVTRPDAPAGRGRRLVASPVAERAEEAGIEV
LKPAKPKDPEFLERLREIGPDCCPVVAYGALLPRVALDVPAHGWVNLHFSLLPAWRGAAP
VQHAIMAGDQITGASTFLIEEGLDSGPVYGTVTEEIRATDTSGDLLTRLAFAGAGLLAAT
MDGIEDGTLKAVPQPAEGVSLAPKVNVEDAHVDWNAPALRVDRLVRGCTPAPGAWTTFRG
ERLKLIQVAAVPDRTDLAPGQIAAQKNNVYVGTGSYGVELLWVQAQGKKPMRAADWARGV
RIAEEERLGG
selected fasta
>putative methionyl-tRNA formyltransferase [putative methionyl-tRNA formyltransferase]
ATGAAGCTCGTCTTCGCCGGTACACCCGAGGTCGCCGTTCCCGCTCTGGACGCCCTTCTC
GCCTCCGGGCGGCACGAGGTGGCCGCCGTCGTCACGCGGCCCGACGCGCCGGCCGGGCGG
GGGCGCAGGCTCGTCGCGTCGCCCGTCGCCGAGCGTGCGGAGGAGGCGGGGATCGAGGTG
CTGAAGCCCGCGAAGCCGAAGGACCCCGAGTTCCTGGAGCGGCTGCGCGAGATCGGGCCG
GACTGCTGTCCCGTCGTCGCCTACGGTGCGCTGCTGCCCAGGGTCGCTCTCGACGTCCCC
GCGCACGGCTGGGTCAATCTGCACTTCTCCCTGCTGCCGGCCTGGCGGGGTGCCGCGCCC
GTGCAGCACGCCATCATGGCGGGCGACCAGATCACCGGGGCGTCGACCTTCCTGATCGAG
GAGGGGCTCGACTCGGGGCCGGTGTACGGGACGGTGACCGAGGAGATCCGGGCCACCGAC
ACCAGCGGGGATCTGCTGACGCGGCTCGCGTTCGCCGGTGCCGGGCTGCTCGCGGCCACC
ATGGACGGGATCGAGGACGGCACCCTGAAGGCCGTACCGCAGCCCGCCGAGGGGGTCAGC
CTGGCCCCGAAGGTCAACGTCGAGGACGCCCACGTCGACTGGAACGCGCCCGCGCTGCGC
GTCGACCGGTTGGTGCGGGGCTGCACTCCGGCACCGGGCGCCTGGACCACGTTCCGCGGT
GAGCGGCTCAAGCTCATCCAGGTCGCCGCCGTGCCGGACCGCACGGATCTCGCGCCGGGG
CAGATCGCCGCCCAGAAGAACAACGTGTACGTCGGCACCGGCTCGTACGGCGTCGAACTG
CTGTGGGTGCAGGCCCAGGGCAAGAAGCCGATGCGGGCGGCGGACTGGGCACGAGGCGTG
CGGATCGCCGAGGAGGAGCGGCTCGGGGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002376 Formyl transferase, N-terminal (Domain)
[1-174] 1.8e-31 PF00551
PF00551   Formyl_trans_N
[1-207] 5.8999880940569e-62 SSF53328
SSF53328   formyl_transf
[1-205] 9.9e-61 G3DSA:3.40.50.170
G3DSA:3.40.50.170   Formyl_transf_N
IPR005793 Formyl transferase, C-terminal (Domain)
[205-300] 9e-25 PF02911
PF02911   Formyl_trans_C
[208-309] 1.4e-31 G3DSA:3.10.25.10
G3DSA:3.10.25.10   Formyl_trans_C
IPR005794 Methionyl-tRNA formyltransferase (Family)
[1-301] 1.39999999999997e-69 TIGR00460
TIGR00460   Fmt
IPR011034 Formyl transferase, C-terminal-like (Domain)
[208-308] 7.00000734129907e-29 SSF50486
SSF50486   FMT_C_like
SignalP
[1-29] 0.207 Signal
Eukaryota   
TMHMM No significant hit
Stref_00330 Stref_00330   Stref_00350 Stref_00350
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