Stref_00340 Stref_00340   Stref_00360 Stref_00360

Stref_00350 : CDS information

close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction40,935 / 41,486 / + [in whole cluster]
40,935 / 41,486 / + [in contig]
Location40935..41486 [in whole cluster]
40935..41486 [in contig]
TypeCDS
Length552 bp (183 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note
  • This ORF is not involved in steffimycin biosynthesis.
Note (GenBank)
  • ORF35
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
comment
steffimycin生合成gene clusterのクローニング、特徴づけ。

36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連し、一次代謝関連genesとhousekeeping genesを含む12ORFsによって挟まれている。

orf1-4, 33-36は、S. coelicolor A3(2) and S. avermitilis MA-4680由来の関連タンパクと有意な類似性あり。遺伝子構成も同じ。

orf31-36を含まない異種性発現株S.albus strain STFaRHAMでsteffimycinの生合成ができている。

close this sectionSequence

selected fasta
>hypothetical protein [hypothetical protein]
MADHATHDAQARASLHLLVRDIERVRRQVDALRTLTAQLGNVYRPRRSGPSTGFVVYGRA
PAPTVRLAQELRDSVETLVTAAVDFDRSLGFSWDAVGSALGVTKQAVHRRYGARRATAQS
AAAAAEAERTAEASGSRTVHVNTGLPAVPTVPAARSMPTQPTAGSPALRDEARPTALPSP
RNG
selected fasta
>hypothetical protein [hypothetical protein]
ATGGCCGACCACGCAACCCACGACGCCCAGGCTCGGGCCAGCCTGCACTTGCTGGTGCGG
GACATCGAGCGGGTCCGCCGGCAGGTGGACGCACTGCGCACGCTCACCGCCCAGCTGGGC
AACGTCTACCGCCCGCGCCGCTCCGGCCCCTCCACGGGCTTCGTCGTCTACGGACGCGCC
CCCGCCCCGACGGTACGTCTCGCGCAGGAACTGCGGGACAGCGTCGAGACCCTGGTCACG
GCAGCCGTGGACTTCGACCGCTCACTCGGCTTCTCGTGGGACGCCGTGGGCTCCGCCCTG
GGAGTCACCAAGCAGGCGGTCCACCGCCGTTACGGCGCACGCCGTGCCACGGCCCAGTCG
GCCGCAGCCGCGGCCGAGGCGGAGCGCACCGCGGAAGCGTCGGGCAGCCGCACGGTCCAT
GTGAACACCGGTCTGCCCGCCGTACCCACGGTCCCCGCGGCCCGCTCCATGCCCACCCAG
CCGACAGCAGGCAGCCCGGCCCTCCGCGACGAGGCGAGGCCGACGGCCCTGCCGAGCCCG
CGCAACGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro No significant hit
SignalP No significant hit
TMHMM No significant hit
Stref_00340 Stref_00340   Stref_00360 Stref_00360
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