Stref_00360 : CDS information

Location

Organism	Streptomyces steffisburgensis
Strain	NRRL 3193 (=NBRC 13446)
Entry name	Steffimycin
Contig	AM156932
Start / Stop / Direction	42,876 / 41,608 / - [in whole cluster] 42,876 / 41,608 / - [in contig]
Location	complement(41608..42876) [in whole cluster] complement(41608..42876) [in contig]
Type	CDS
Length	1,269 bp (422 aa)

Annotation

Category
Product
Product (GenBank)	putative primosomal protein N'
Gene
Gene (GenBank)
EC number
Keyword
Note	This ORF is not involved in steffimycin biosynthesis.
Note (GenBank)	ORF36
Reference	ACC Q2P9X9 PmId [16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006) comment steffimycin生合成gene clusterのクローニング、特徴づけ。 36ORFsが見つかり、そのうち24ORFsを含む26.5 kb領域がおそらくsteffimycin生合成に関連し、一次代謝関連genesとhousekeeping genesを含む12ORFsによって挟まれている。 orf1-4, 33-36は、S. coelicolor A3(2) and S. avermitilis MA-4680由来の関連タンパクと有意な類似性あり。遺伝子構成も同じ。 orf31-36を含まない異種性発現株S.albus strain STFaRHAMでsteffimycinの生合成ができている。

Sequence

>[putative primosomal protein N']
MGTRAAMFAPVQDLGLVALWDDGDDSHSEQHAPQPHAREVLLLRAAQDKCGFLLGSWGCT
VEAAQLVETGWARPLVATRERVRATAPLVRTVGDGDLARDEAARAARLPTLAWQAVRDGL
AVGPVLVQVPRRGYVPRMACAVCRAPARCPHCSGPLQARDGGALDCGWCGREEAGWHCPE
CGSFRLRASVVGARRTAEELGRAFPTVPVRTSGREHVLDTVPGAPALVVSTPGAEPVAEG
GYAAALLLDGWAMLGRPDLRAGEDALRRWIAAGALVRPQSDGGTVVVVAEPTLRPVQALV
RWDPVGHAVRELAERAELGFPPVSRMAAVSGPPEAVADFLRTAELPPEAEVLGPVPLPVP
AAGRPRRAGAPPPGEQWERALLRVPPGRGAALAAALKAAQAARMARGSGSAVWVRIDPVD
IG

>[putative primosomal protein N']
ATCGGCACCCGGGCGGCGATGTTCGCGCCGGTCCAGGACCTGGGACTCGTCGCCCTGTGG
GACGACGGCGACGACAGCCACAGCGAGCAGCACGCCCCGCAGCCGCACGCCCGGGAGGTG
CTGCTGCTGCGGGCCGCCCAGGACAAGTGCGGCTTCCTGCTGGGCAGCTGGGGCTGCACG
GTGGAGGCCGCGCAACTCGTCGAGACCGGGTGGGCGCGGCCGCTGGTCGCCACGCGGGAG
CGGGTACGCGCCACCGCACCGCTCGTACGGACCGTCGGTGACGGGGACCTCGCGCGGGAC
GAGGCGGCCCGGGCCGCCCGGCTGCCGACGCTCGCCTGGCAGGCCGTCAGGGACGGTCTG
GCCGTGGGCCCGGTGCTGGTGCAGGTGCCCCGGCGGGGCTACGTGCCGCGCATGGCGTGC
GCCGTCTGCCGGGCGCCCGCGCGGTGCCCGCACTGCTCCGGGCCGCTCCAGGCGCGGGAC
GGCGGTGCCCTGGACTGCGGGTGGTGCGGCCGCGAGGAGGCCGGCTGGCACTGCCCCGAG
TGCGGTTCCTTCCGGCTGCGCGCCTCGGTGGTGGGTGCGCGGCGGACCGCCGAGGAACTC
GGGCGGGCCTTCCCCACGGTTCCCGTGCGGACGTCCGGCCGTGAACACGTGCTCGACACG
GTGCCGGGGGCGCCCGCGCTGGTGGTCAGTACGCCGGGGGCCGAGCCCGTCGCCGAGGGC
GGGTACGCGGCGGCGCTGCTGCTCGACGGGTGGGCCATGCTCGGGCGGCCGGATCTGCGG
GCCGGCGAGGACGCGTTGCGGCGGTGGATCGCGGCCGGGGCACTCGTCCGGCCGCAGTCG
GACGGGGGCACGGTGGTGGTCGTGGCCGAGCCGACGCTGCGGCCCGTGCAGGCGCTGGTG
CGGTGGGACCCCGTGGGGCACGCGGTGCGCGAGCTGGCCGAGCGCGCCGAGCTGGGATTT
CCGCCGGTGTCGCGGATGGCGGCCGTGTCGGGGCCGCCGGAAGCGGTGGCCGACTTCCTC
CGTACGGCCGAACTGCCGCCGGAGGCCGAGGTGCTGGGGCCGGTGCCGTTGCCCGTCCCC
GCCGCGGGGCGACCGCGGCGGGCGGGAGCACCGCCGCCCGGGGAGCAGTGGGAGCGGGCG
CTGCTGCGGGTGCCGCCTGGGAGGGGTGCCGCGCTGGCCGCCGCGCTGAAGGCGGCGCAG
GCGGCGCGAATGGCTCGGGGGAGTGGGAGCGCGGTGTGGGTGCGGATCGACCCCGTGGAC
ATCGGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Stref_00350 last last