first first   Strply_00020 Strply_00020

Strply_00010 : CDS information

close this sectionLocation

Organism
StrainNRRL 2433 (=NBRC 13058)
Entry nameStreptolydigin
Contig
Start / Stop / Direction1 / 438 / + [in whole cluster]
1 / 438 / + [in contig]
Location1..438 [in whole cluster]
1..438 [in contig]
TypeCDS
Length438 bp (145 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative glycerophosphodiester phosphodiesterase(fragment)
Product (GenBank)putative glycerophosphoryl diester phosphodiesterase
Gene
Gene (GenBank)
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in streptolydigin biosynthesis.
Note (GenBank)
  • ORF1
Reference
ACC
PmId
[19875077] Deciphering biosynthesis of the RNA polymerase inhibitor streptolydigin and generation of glycosylated derivatives. (Chem Biol. , 2009)
comment
streptolydigin生合成gene clusterの報告。

ORF1-6は相同性を示すSCO,SAVと同様の機能を果たすと考察している。
ORF1は、下記と相同性を示す。
Q82N11(SAV1492):Putative glycerophosphoryl diester phosphodiesterase
Q9K3Q8(SCO1090):Putative phosphodiesterase


streptolydigin生合成領域を特定するために、orf2から上流および orf3から下流領域の不活化株を作製。いずれも streotolydiginの生産量が変化しなかった(data not shown)ことから、Orf2から上流およびOrf3から下流領域は streptolydiginの生合成には関与していないと考察している。
この領域は、一次代謝やhousekeepingに関与しているのだろうと推測している。


本ORFは機能解析実験はされていない。
Family/Domain
FD
IPR004129
comment
[IPR004129] Glycerophosphoryl diester phosphodiesterase (Family)

close this sectionSequence

selected fasta
>putative glycerophosphodiester phosphodiesterase(fragment) [putative glycerophosphoryl diester phosphodiesterase]
GSVFEQVVEAVKAPLQAEIKDVAAAQALAEVMRARDLVGRVEVISFHDEALAAIRTLLPG
VRTALVASRYGADVVDRAQAVGATMLSLNIRRLTLELVERAHAAHLKVVAWTVNTHDQLR
LARGLGLDGVVTDLPEIRRAVRFTA
selected fasta
>putative glycerophosphodiester phosphodiesterase(fragment) [putative glycerophosphoryl diester phosphodiesterase]
GGATCCGTCTTCGAGCAGGTGGTGGAGGCGGTCAAGGCGCCGCTCCAGGCCGAGATCAAG
GACGTGGCGGCCGCGCAGGCGCTGGCCGAGGTGATGCGGGCGCGCGATCTGGTCGGCCGG
GTCGAGGTGATCTCCTTCCACGACGAGGCGCTGGCGGCGATCCGCACCCTGCTGCCGGGG
GTGCGCACCGCGCTGGTGGCCAGTCGCTACGGCGCCGATGTGGTGGACCGCGCCCAGGCC
GTGGGCGCGACGATGCTCTCGCTGAACATCCGGCGGCTCACCCTGGAACTGGTCGAGCGG
GCGCACGCCGCCCATCTGAAGGTCGTCGCCTGGACGGTCAACACCCACGACCAGCTGCGA
CTGGCGCGCGGCCTGGGGCTGGACGGTGTGGTGACCGACCTCCCGGAGATCCGGCGGGCG
GTGCGCTTCACCGCGTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004129 Glycerophosphoryl diester phosphodiesterase (Family)
[39-136] 1.3e-10 PF03009
PF03009   GDPD
IPR017946 PLC-like phosphodiesterase, TIM beta/alpha-barrel domain (Domain)
[4-142] 1.29999924468179e-22 SSF51695
SSF51695   PLC-like_Pdiesterase_TIM-brl
[4-140] 2.4e-26 G3DSA:3.20.20.190
G3DSA:3.20.20.190   PLC-like_Pdiesterase_TIM-brl
SignalP
[1-24] 0.138 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
first first   Strply_00020 Strply_00020
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