Strply_00190 : CDS information

Location

Organism	Streptomyces lydicus
Strain	NRRL 2433 (=NBRC 13058)
Entry name	Streptolydigin
Contig	FN433113
Start / Stop / Direction	24,499 / 23,432 / - [in whole cluster] 24,499 / 23,432 / - [in contig]
Location	complement(23432..24499) [in whole cluster] complement(23432..24499) [in contig]
Type	CDS
Length	1,068 bp (355 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	NDP-glucose synthase
Gene
Gene (GenBank)	slgS1
EC number	2.7.7.24
Keyword	L-rhodinose
Note
Note (GenBank)
Reference	ACC D1GLU7 PmId [19875077] Deciphering biosynthesis of the RNA polymerase inhibitor streptolydigin and generation of glycosylated derivatives. (Chem Biol. , 2009) comment streptolydigin生合成gene clusterの報告。本ORF上流の挿入変異株の解析より、本ORFはstreptolydigin生産に関与する領域であることが示されている。 L-rhodinose biosynthesisに関与するORFと予測されている。本ORFの機能解析実験はされていない。
Related Reference	ACC Q194R4 NITE Mith_00060 PmId [9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997) comment Streptomyces argillaceus_dTDP-glucose synthase(mtmD) E. coli発現タンパクで以下の2酵素の活性みている。 mtmD: glucose-1-phosphate:dTTP thymidylyl transferase mtmE: dTDP-D-glucose 4,6-dehydratase mtmD mutant -- accumulates premithramycinone

Sequence

>putative glucose-1-phosphate thymidylyltransferase [NDP-glucose synthase]
MRALVLAGGTGTRLRPLTHTSAKQMVPVANKPVLFYGLESIARAGIIDVGIVVGDTAAEI
RAAVGDGSRFGLKVTYIPQHAPLGLAHAVLVARDFLGEEDFLMYLGDNFICGGITGLVAD
FQVKRPDAHILLTRVADPGAFGVAELGPDGRVVGLEEKPEQPRSDLAMVGVYLFTPAVHE
AVRAIEPSRRGELEITHALQWLIDAGRRVDSTLIEDYWKDTGSVVDMLEVNRTVLESAEP
RLDGLVDEHSEIIGRVVVEAGSQIRGSRIVGPVVIGADSRITGSYVGPFTSIGERCRLDD
CEVEYSVVLPETRITGVRRIAASLIGRNVEVSPAAPGPRTHRLVLGDHSTVQIAS

>putative glucose-1-phosphate thymidylyltransferase [NDP-glucose synthase]
ATGAGAGCACTCGTGCTGGCCGGCGGGACCGGGACCAGACTGCGTCCGCTCACCCACACC
TCGGCCAAGCAGATGGTGCCGGTGGCCAACAAGCCCGTGCTGTTCTACGGCCTGGAATCG
ATCGCCCGTGCCGGGATCATCGACGTCGGCATCGTCGTCGGCGACACCGCGGCGGAGATC
CGCGCGGCGGTCGGGGACGGATCGAGATTCGGCCTGAAGGTCACCTACATCCCGCAGCAC
GCGCCGCTCGGCCTGGCCCACGCGGTGCTGGTCGCCCGGGACTTCCTGGGGGAGGAGGAC
TTCCTCATGTACCTCGGCGACAACTTCATCTGCGGCGGGATCACCGGACTCGTGGCGGAC
TTCCAGGTCAAACGGCCCGACGCACACATCCTGCTGACCCGGGTGGCCGACCCCGGCGCC
TTCGGCGTGGCCGAGCTCGGCCCGGACGGGCGGGTCGTGGGGCTGGAGGAGAAGCCGGAG
CAGCCGCGCAGCGACCTCGCCATGGTCGGCGTCTACCTGTTCACCCCGGCCGTCCACGAA
GCGGTGCGGGCCATCGAGCCCTCCCGGCGGGGCGAGCTGGAGATCACCCACGCCCTGCAA
TGGCTGATCGACGCCGGGCGGCGGGTGGACTCCACCCTCATCGAGGACTACTGGAAGGAC
ACCGGTAGCGTGGTGGACATGCTGGAGGTCAACCGCACCGTCCTGGAGAGCGCCGAACCC
AGGCTGGACGGGCTGGTCGACGAGCACAGCGAGATCATCGGCCGGGTCGTGGTCGAGGCC
GGGTCCCAGATCCGCGGCTCACGCATCGTCGGCCCGGTCGTGATCGGCGCGGACAGCCGG
ATCACCGGCTCCTACGTCGGTCCGTTCACCTCCATCGGCGAGCGCTGCCGGCTCGACGAC
TGCGAGGTCGAGTACTCCGTCGTCCTGCCGGAGACCCGCATCACCGGAGTGCGCCGCATC
GCCGCCTCGCTGATCGGACGCAATGTGGAGGTCAGCCCGGCCGCGCCCGGCCCCCGCACC
CACCGCCTCGTCCTCGGCGACCACAGCACCGTGCAGATCGCGTCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family) TIGR01208 RmlA_long
SignalP	No significant hit
TMHMM	No significant hit

Strply_00180 Strply_00200