Tatmc_00010 : CDS information

Location

Organism	Streptomyces sp.
Strain	CK4412
Entry name	Tautomycetin
Contig	DQ983361
Start / Stop / Direction	2,908 / 1,385 / - [in whole cluster] 2,908 / 1,385 / - [in contig]
Location	complement(1385..2908) [in whole cluster] complement(1385..2908) [in contig]
Type	CDS
Length	1,524 bp (507 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	TMC biosynthetic enzyme L7
Gene	tmcI
Gene (GenBank)
EC number
Keyword
Note	This ORF is not involved in tautomycetin biosynthesis.
Note (GenBank)	hypothetical protein; ORF L7
Reference	ACC A4KCF1 PmId [17379718] Isolation of the biosynthetic gene cluster for tautomycetin, a linear polyketide T cell-specific immunomodulator from Streptomyces sp. CK4412. (Microbiology. , 2007) comment tautomycetinの生合成遺伝子クラスターに関する文献。 tmcI: hypothetical protein (Nnocardia farcinica), id=34% polyprenyl phosphomannosyltransferase MppI (Streptomyces hygroscopicus), id=33% tmcI自体の機能実験は行われていない。
Related Reference	ACC C6ZCS8 PmId [19191560] Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes provides new insight into dialkylmaleic anhydride biosynthesis. (J Nat Prod. , 2009) comment <Putative glycosyl transferase, Streptomyces griseochromogenes, BLAST 2; id=100%> Streptomyces griseochromogenesにおけるTautomycetin Biosynthetic Gene Clusterを同定した文献。 orf1 (507 a.a): polyprenyl phospho-mannosyltransferase orf1自体の機能実験は行われていないが、orf1の不活化変異株はTautomycetin生合成に少しも影響を与えなかったので、Tautomycetin生合成に関係無いとしている。

Sequence

>hypothetical protein [TMC biosynthetic enzyme L7]
MTEQVSAAAPHYEAPLVIGTEATPPIAWRPVLITAAVVAAVITAVSWRVGAYSNELYFIG
AGGHPAWGYVDQPPLTPVLAHGLWSVAHSLVLLRLPATLCMVAMVVLSALTARELGGERR
AQTVAALTTATSILLLSLSATLATTAIDSALWATVLWLVARWNRLRQDRLLLVATLVTAV
ALENKYLIVSLWAVLIVAVLVSGPRELLRRPLLWVGGAVAILSAVPGLLWQASHGWPQIE
MTSVIAAEAQRDAGGRLGFLPLVLFYAGIPVGVALAVRGVVRLWRIPAHRFLGITVGGVV
LAFLIADGRHTYTGGLLPLLFAAGSINLRGRVWRVVAGAPGLILAALIAVPLSLSVLPVS
TGAQVNILTRGSTGWPELVDTVAAAYRSAGPGPVALVADTYRQAGALDAYGESRGLPKAS
SPDRGYSAFGPPADEVTTVLWVGDRPPAEFRAAFAEVREVARFDDNPDGYLGINSDVPVW
LCTGFHDDWQQLWPRIKHYDSRDRVAA

>hypothetical protein [TMC biosynthetic enzyme L7]
ATGACCGAGCAAGTCAGCGCCGCCGCGCCGCACTACGAGGCCCCACTGGTCATCGGCACC
GAAGCCACGCCGCCGATCGCCTGGCGGCCGGTGCTCATCACCGCGGCGGTGGTCGCGGCC
GTGATCACCGCGGTCAGCTGGCGGGTCGGCGCGTACAGCAACGAGCTCTACTTCATCGGC
GCGGGCGGCCATCCGGCCTGGGGCTACGTGGACCAGCCGCCGCTCACCCCGGTGCTGGCC
CACGGCTTGTGGTCCGTCGCGCACTCGCTCGTGCTGCTGCGGCTGCCGGCCACCCTGTGC
ATGGTCGCCATGGTCGTGTTGAGCGCGCTCACCGCGCGCGAGTTGGGCGGTGAGCGCCGT
GCCCAGACGGTGGCGGCCCTGACCACGGCCACGTCGATCCTGCTGCTGTCGCTGAGCGCC
ACGCTGGCGACGACGGCGATCGACTCGGCGCTGTGGGCGACGGTGCTGTGGCTGGTCGCC
CGCTGGAACCGCCTGCGCCAGGACCGGCTGCTGCTGGTCGCCACCCTGGTCACCGCGGTG
GCACTGGAGAACAAGTACCTGATCGTCTCGCTGTGGGCCGTCCTGATCGTCGCCGTGCTC
GTCTCCGGCCCGCGGGAACTCCTGCGCCGCCCGCTGCTGTGGGTCGGTGGCGCCGTGGCG
ATCCTCAGCGCCGTGCCGGGTCTGCTCTGGCAGGCGAGCCACGGCTGGCCGCAGATCGAG
ATGACGAGCGTCATCGCGGCCGAGGCGCAGCGGGACGCCGGTGGGCGGCTCGGCTTCCTG
CCGCTCGTGCTCTTCTACGCCGGAATCCCAGTCGGTGTGGCCCTGGCGGTCCGCGGCGTC
GTGCGGCTGTGGCGCATCCCGGCCCACCGCTTCCTCGGCATCACCGTCGGCGGCGTTGTC
CTGGCCTTCCTGATCGCGGACGGCCGGCACACGTACACCGGCGGGCTGTTGCCCCTGCTC
TTCGCCGCGGGTTCGATCAATCTGAGAGGGCGCGTGTGGCGGGTGGTCGCCGGCGCCCCC
GGGCTGATCCTGGCGGCGCTCATCGCCGTGCCGCTGTCACTGTCGGTGCTCCCGGTGTCG
ACCGGGGCGCAGGTCAACATCCTGACCCGGGGCAGCACCGGCTGGCCGGAACTCGTCGAC
ACCGTCGCCGCGGCTTACCGGTCGGCGGGTCCGGGCCCGGTGGCCCTGGTCGCCGACACC
TACCGCCAGGCAGGTGCCCTGGACGCGTACGGGGAATCGCGCGGCCTGCCGAAGGCCAGC
AGCCCGGACCGCGGCTACTCCGCCTTCGGCCCGCCCGCGGACGAGGTGACCACGGTGCTG
TGGGTCGGAGACAGGCCCCCGGCCGAATTCCGTGCCGCGTTCGCCGAGGTGCGTGAGGTA
GCCCGCTTCGACGACAACCCGGACGGCTACCTCGGCATCAACTCCGACGTTCCGGTGTGG
CTGTGCACCGGATTCCACGATGACTGGCAGCAGCTGTGGCCGCGAATCAAGCACTACGAC
AGCCGGGACCGCGTCGCCGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Eukaryota
TMHMM	Transmembrane 9

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