Tauto_00200 Tauto_00200   Tauto_00220 Tauto_00220

Tauto_00210 : CDS information

close this sectionLocation

Organism
Strain
Entry nameTautomycin
Contig
Start / Stop / Direction86,805 / 85,861 / - [in whole cluster]
86,805 / 85,861 / - [in contig]
Locationcomplement(85861..86805) [in whole cluster]
complement(85861..86805) [in contig]
TypeCDS
Length945 bp (314 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productputative lyase
Product (GenBank)probable citrate lyase beta subunit
Gene
Gene (GenBank)ttmO
EC number
Keyword
  • dialkylmaleic anhydride moiety
Note
Note (GenBank)
Reference
ACC
PmId
[18708355] Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. (J Biol Chem. , 2008)
comment
tautomycin (TTM) biosynthetic gene clusterについての文献。

ttmO(314a.a.): citryl CoA lyase

ttmOの機能実験は無いが、Mycobacterium tuberculosisのcitryl-CoA lyase (CitE)との相同性より、alpha-ketoglutarateのalpha-carbonyl groupとmethylmalonyl-CoAとのcondensationに関与していると提唱している。
Related Reference
ACC
P17725
PmId
[7830578] Klebsiella pneumoniae genes for citrate lyase and citrate lyase ligase: localization, sequencing, and expression. (Mol Microbiol. , 1994)
comment
<Citrate lyase subunit beta, Klebsiella pneumoniae, BLAST 129; id=33%>

Klebsiella pneumoniaeよりcitD(gammma-), citE(beta-) and citF(alpha-subunit of citrate lyase) の遺伝子をクローニングしてシークエンスしており、これらの遺伝子がcitC(citrate lyase ligase）、citG(機能不明）とともにcitCDEFG gene clusterを形成していることを示している。また、citCDEFGを大腸菌で発現させたところ、acetyl-CoA存在下でのみcitrate lyase活性が検出されたことが示されている。
Family/Domain
FD
IPR005000
comment
[IPR005000] HpcH/HpaI aldolase (Family)

Function
GO:0016830 carbon-carbon lyase activity

close this sectionSequence

selected fasta
>putative lyase [probable citrate lyase beta subunit]
MTDDHGKHRVFRSCLYVPGHHPDRIVRAYDTEADAVILDLEDAVPAPRKPEAREIVAEAM
ATPAAKPTYVRVNPMSSGLCEDDIRAVAGPALAGVRVAKVDSPADVLRVAEVLRELGHPA
KIHVLIESAVALEHAFALATASPEVTMLGLGESDLRVDLSTTAEGPTMDTSRARVIVAAR
AAGLPNPCQSVYAEPRDLRGLLVTSKHGRRLGFLGRMAIHPAQIPVIHDVYTPTTNEIDD
ALEICAAVNLAALESRSITITSQGKMVGPPVLARAKQVLELATALNLITDAAWPVEQART
SESVGYIRNKGGSS
selected fasta
>putative lyase [probable citrate lyase beta subunit]
ATGACCGACGACCACGGAAAGCACCGGGTGTTCCGCAGCTGCCTGTACGTGCCGGGCCAC
CACCCGGACCGGATCGTGCGCGCCTACGACACGGAGGCCGACGCGGTGATACTCGACCTG
GAGGACGCCGTCCCGGCCCCGCGCAAGCCCGAGGCTCGTGAGATCGTCGCCGAGGCCATG
GCCACGCCGGCCGCGAAGCCCACCTACGTGCGGGTCAACCCCATGTCGTCGGGCCTGTGC
GAGGACGACATCCGCGCGGTCGCGGGTCCCGCGCTCGCCGGGGTGCGGGTGGCCAAAGTG
GACTCGCCCGCCGACGTCCTGCGCGTCGCCGAGGTGCTGCGGGAGCTGGGACACCCGGCG
AAGATCCATGTGCTCATCGAATCGGCCGTCGCGCTGGAACACGCGTTCGCCCTGGCCACC
GCGTCGCCCGAGGTGACGATGCTCGGCCTCGGCGAGTCCGATCTCCGGGTCGACCTCAGC
ACCACCGCCGAGGGGCCGACCATGGACACCAGCCGGGCCCGCGTGATCGTCGCCGCGCGT
GCGGCCGGCCTGCCCAATCCGTGCCAGAGCGTGTACGCCGAGCCCCGCGATCTGCGCGGG
CTGCTGGTCACCAGCAAGCACGGCCGCCGGCTCGGGTTCCTCGGTCGCATGGCGATCCAC
CCGGCGCAGATCCCCGTCATCCACGACGTCTACACCCCGACCACGAACGAGATCGACGAC
GCGCTGGAGATCTGCGCCGCAGTGAACCTCGCAGCGCTGGAGAGCAGGTCGATCACCATC
ACGTCGCAGGGGAAGATGGTCGGCCCGCCCGTGCTCGCCCGGGCGAAGCAGGTCCTTGAG
CTGGCCACCGCGCTCAACCTGATCACGGATGCCGCGTGGCCGGTGGAGCAGGCACGAACT
TCGGAATCGGTGGGTTACATCCGGAACAAAGGAGGTTCGTCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005000 Aldehyde-lyase domain (Domain)
[12-221] 4.80000000000001e-32 PF03328
PF03328   HpcH_HpaI
IPR011206 Citrate lyase, beta subunit (Family)
[1-289] 5.50001754266469e-73 PIRSF015582
PIRSF015582   Cit_lyase_B
IPR015813 Pyruvate/Phosphoenolpyruvate kinase (Domain)
[8-232] 1.99999999999999e-67 G3DSA:3.20.20.60
G3DSA:3.20.20.60   Pyrv/PenolPyrv_Kinase_cat
[7-232] 3.49999466863949e-60 SSF51621
SSF51621   Pyrv/PenolPyrv_Kinase_cat
SignalP No significant hit
TMHMM No significant hit
Tauto_00200 Tauto_00200   Tauto_00220 Tauto_00220
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